Evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis
Non-infectious anterior uveitis (AU) is a potentially sight threatening inflammatory condition. The current gold standard for treatment is topical steroids, but low ocular bioavailability and compliance issues with the intensive dosing regimen limit the efficacy of this treatment. Liposomes as a dru...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/87423 http://hdl.handle.net/10220/45387 |
| _version_ | 1824456095168462848 |
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| author | Wong, Chee Wai Czarny, Bertrand Metselaar, Josbert M. Ho, Candice Ng, Si Rui Barathi, Amutha Veluchamy Storm, Gert Wong, Tina T. |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Wong, Chee Wai Czarny, Bertrand Metselaar, Josbert M. Ho, Candice Ng, Si Rui Barathi, Amutha Veluchamy Storm, Gert Wong, Tina T. |
| author_sort | Wong, Chee Wai |
| collection | NTU |
| description | Non-infectious anterior uveitis (AU) is a potentially sight threatening inflammatory condition. The current gold standard for treatment is topical steroids, but low ocular bioavailability and compliance issues with the intensive dosing regimen limit the efficacy of this treatment. Liposomes as a drug delivery system may help to overcome these problems. We studied the efficacy of a PEG-liposomal formulation of liposomal steroids, administered as a single subconjunctival dose, in the treatment of experimental uveitis in rabbit eyes. Rabbits that received subconjunctival liposomal triamcinolone acetonide phosphate (LTAP) or liposomal prednisolone phosphate (LPP) had significantly lower mean inflammatory scores than untreated controls on Day 4 after induction of uveitis (LPP vs controls, p=0.049) and 8 (LPP vs controls, p=0.007; LTAP vs controls, p=0.019), and lower scores than rabbits given topical PredForte1% 4 times a day on Day 8 (p=0.03). After antigen rechallenge, the subconjunctival liposomal steroid groups continued to have greater suppression of inflammation than untreated controls on Day 11 (p=0.02). Localization of liposomes in inflamed ocular tissue was confirmed by histology and immunostaining, and persisted in the eye for at least one month. Our study demonstrates that a single subconjunctival injection of liposomal steroids induces effective and sustained anti-inflammatory action. |
| first_indexed | 2025-02-19T03:48:39Z |
| format | Journal Article |
| id | ntu-10356/87423 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:48:39Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/874232023-07-14T15:45:48Z Evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis Wong, Chee Wai Czarny, Bertrand Metselaar, Josbert M. Ho, Candice Ng, Si Rui Barathi, Amutha Veluchamy Storm, Gert Wong, Tina T. School of Materials Science & Engineering Lee Kong Chian School of Medicine (LKCMedicine) Anterior Uveitis (AU) Liposomal Steroids Non-infectious anterior uveitis (AU) is a potentially sight threatening inflammatory condition. The current gold standard for treatment is topical steroids, but low ocular bioavailability and compliance issues with the intensive dosing regimen limit the efficacy of this treatment. Liposomes as a drug delivery system may help to overcome these problems. We studied the efficacy of a PEG-liposomal formulation of liposomal steroids, administered as a single subconjunctival dose, in the treatment of experimental uveitis in rabbit eyes. Rabbits that received subconjunctival liposomal triamcinolone acetonide phosphate (LTAP) or liposomal prednisolone phosphate (LPP) had significantly lower mean inflammatory scores than untreated controls on Day 4 after induction of uveitis (LPP vs controls, p=0.049) and 8 (LPP vs controls, p=0.007; LTAP vs controls, p=0.019), and lower scores than rabbits given topical PredForte1% 4 times a day on Day 8 (p=0.03). After antigen rechallenge, the subconjunctival liposomal steroid groups continued to have greater suppression of inflammation than untreated controls on Day 11 (p=0.02). Localization of liposomes in inflamed ocular tissue was confirmed by histology and immunostaining, and persisted in the eye for at least one month. Our study demonstrates that a single subconjunctival injection of liposomal steroids induces effective and sustained anti-inflammatory action. Published version 2018-07-30T07:49:38Z 2019-12-06T16:41:33Z 2018-07-30T07:49:38Z 2019-12-06T16:41:33Z 2018 Journal Article Wong, C. W., Czarny, B., Metselaar, J. M., Ho, C., Ng, S. R., Barathi, A. V., et al. (2018). Evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis. Scientific Reports, 8(1), 6604-. 2045-2322 https://hdl.handle.net/10356/87423 http://hdl.handle.net/10220/45387 10.1038/s41598-018-24545-2 en Scientific Reports © 2018 The Author(s) (Nature Publishing Group). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 11 p. application/pdf |
| spellingShingle | Anterior Uveitis (AU) Liposomal Steroids Wong, Chee Wai Czarny, Bertrand Metselaar, Josbert M. Ho, Candice Ng, Si Rui Barathi, Amutha Veluchamy Storm, Gert Wong, Tina T. Evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis |
| title | Evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis |
| title_full | Evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis |
| title_fullStr | Evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis |
| title_full_unstemmed | Evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis |
| title_short | Evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis |
| title_sort | evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis |
| topic | Anterior Uveitis (AU) Liposomal Steroids |
| url | https://hdl.handle.net/10356/87423 http://hdl.handle.net/10220/45387 |
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