Semiconducting polymer nanobiocatalysts for photoactivation of intracellular redox reactions
An organic semiconducting polymer nanobiocatalyst (SPNB) composed of a semiconducting polymer core conjugated with microsomal cytochrome P450 (CYP) has been developed for photoactivation of intracellular redox. The core serves as the light-harvesting unit to initiate photoinduced electron transfer (...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Online Access: | https://hdl.handle.net/10356/87488 http://hdl.handle.net/10220/47705 |
| _version_ | 1826113818126712832 |
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| author | Lyu, Yan Tian, Jingqi Li, Jingchao Chen, Peng Pu, Kanyi |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Lyu, Yan Tian, Jingqi Li, Jingchao Chen, Peng Pu, Kanyi |
| author_sort | Lyu, Yan |
| collection | NTU |
| description | An organic semiconducting polymer nanobiocatalyst (SPNB) composed of a semiconducting polymer core conjugated with microsomal cytochrome P450 (CYP) has been developed for photoactivation of intracellular redox. The core serves as the light-harvesting unit to initiate photoinduced electron transfer (PET) and facilitate the regeneration of dihydronicotinamide adenine dinucleotide phosphate (NADPH), while CYP is the catalytic center for intracellular
redox. Under light irradiation, the semiconducting core can efficiently catalyze the generation of NADPH with a turnover frequency (TOF) 75 times higher than the reported nanosystems, ensuring the supply of the cofactor for intracellular redox. SPNB-mediated intracellular redox thus can be efficiently activated by light in living cells to convert the model substrate and also to trigger the bioactivation of anticancer drug. This study provides an organic nanobiocatalytic system
that allows light to remotely control intracellular redox in living systems. |
| first_indexed | 2024-10-01T03:29:28Z |
| format | Journal Article |
| id | ntu-10356/87488 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:29:28Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/874882023-12-29T06:46:36Z Semiconducting polymer nanobiocatalysts for photoactivation of intracellular redox reactions Lyu, Yan Tian, Jingqi Li, Jingchao Chen, Peng Pu, Kanyi School of Chemical and Biomedical Engineering Biocatalysts Nanomaterials DRNTU::Engineering::Chemical engineering An organic semiconducting polymer nanobiocatalyst (SPNB) composed of a semiconducting polymer core conjugated with microsomal cytochrome P450 (CYP) has been developed for photoactivation of intracellular redox. The core serves as the light-harvesting unit to initiate photoinduced electron transfer (PET) and facilitate the regeneration of dihydronicotinamide adenine dinucleotide phosphate (NADPH), while CYP is the catalytic center for intracellular redox. Under light irradiation, the semiconducting core can efficiently catalyze the generation of NADPH with a turnover frequency (TOF) 75 times higher than the reported nanosystems, ensuring the supply of the cofactor for intracellular redox. SPNB-mediated intracellular redox thus can be efficiently activated by light in living cells to convert the model substrate and also to trigger the bioactivation of anticancer drug. This study provides an organic nanobiocatalytic system that allows light to remotely control intracellular redox in living systems. MOE (Min. of Education, S’pore) Accepted version 2019-02-19T08:20:38Z 2019-12-06T16:42:58Z 2019-02-19T08:20:38Z 2019-12-06T16:42:58Z 2018 Journal Article Lyu, Y., Tian, J., Li, J., Chen, P., & Pu, K. (2018). Semiconducting polymer nanobiocatalysts for photoactivation of intracellular redox reactions. Angewandte Chemie International Edition, 57(41), 13484-13488. doi:10.1002/anie.201806973 1433-7851 https://hdl.handle.net/10356/87488 http://hdl.handle.net/10220/47705 10.1002/anie.201806973 209109 en Angewandte Chemie International Edition © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved. This paper was published in Angewandte Chemie International Edition and is made available with permission of Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. 6 p. application/pdf |
| spellingShingle | Biocatalysts Nanomaterials DRNTU::Engineering::Chemical engineering Lyu, Yan Tian, Jingqi Li, Jingchao Chen, Peng Pu, Kanyi Semiconducting polymer nanobiocatalysts for photoactivation of intracellular redox reactions |
| title | Semiconducting polymer nanobiocatalysts for photoactivation of intracellular redox reactions |
| title_full | Semiconducting polymer nanobiocatalysts for photoactivation of intracellular redox reactions |
| title_fullStr | Semiconducting polymer nanobiocatalysts for photoactivation of intracellular redox reactions |
| title_full_unstemmed | Semiconducting polymer nanobiocatalysts for photoactivation of intracellular redox reactions |
| title_short | Semiconducting polymer nanobiocatalysts for photoactivation of intracellular redox reactions |
| title_sort | semiconducting polymer nanobiocatalysts for photoactivation of intracellular redox reactions |
| topic | Biocatalysts Nanomaterials DRNTU::Engineering::Chemical engineering |
| url | https://hdl.handle.net/10356/87488 http://hdl.handle.net/10220/47705 |
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