Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement
Particle trapping and binding in optical potential wells provide a versatile platform for various biomedical applications. However, implementation systems to study multi-particle contact interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we demonstrate the precise...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/87504 http://hdl.handle.net/10220/45434 |
| _version_ | 1811681633360674816 |
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| author | Shi, Yu Zhi Xiong, Sha Zhang, Yi Chin, Lip Ket Chen, Yan-Yu Zhang, Jing Bo Ser, Wee Hoi, Lim Siau Liedberg, Bo Yap, Peng Huat Tsai, Din Ping Liu, Ai Qun Zhang, T. H. Larson, A. Wu, J. H. Chen, T. N. Yang, Z. C. Hao, Y. L. Qiu, C.-W. |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Shi, Yu Zhi Xiong, Sha Zhang, Yi Chin, Lip Ket Chen, Yan-Yu Zhang, Jing Bo Ser, Wee Hoi, Lim Siau Liedberg, Bo Yap, Peng Huat Tsai, Din Ping Liu, Ai Qun Zhang, T. H. Larson, A. Wu, J. H. Chen, T. N. Yang, Z. C. Hao, Y. L. Qiu, C.-W. |
| author_sort | Shi, Yu Zhi |
| collection | NTU |
| description | Particle trapping and binding in optical potential wells provide a versatile platform for various biomedical applications. However, implementation systems to study multi-particle contact interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we demonstrate the precise control of particle interactions and functions such as controlling aggregation and multi-hopping. The mean residence time of a single particle is found considerably reduced from 7 s, as predicted by Kramer’s theory, to 0.6 s, owing to the mechanical interactions among aggregated particles. The optofluidic lattice also enables single-bacteria-level screening of biological binding agents such as antibodies through particle-enabled bacteria hopping. The binding efficiency of antibodies could be determined directly, selectively, quantitatively and efficiently. This work enriches the fundamental mechanisms of particle kinetics and offers new possibilities for probing and utilising unprecedented biomolecule interactions at single-bacteria level. |
| first_indexed | 2024-10-01T03:44:03Z |
| format | Journal Article |
| id | ntu-10356/87504 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:44:03Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/875042020-06-01T10:21:27Z Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement Shi, Yu Zhi Xiong, Sha Zhang, Yi Chin, Lip Ket Chen, Yan-Yu Zhang, Jing Bo Ser, Wee Hoi, Lim Siau Liedberg, Bo Yap, Peng Huat Tsai, Din Ping Liu, Ai Qun Zhang, T. H. Larson, A. Wu, J. H. Chen, T. N. Yang, Z. C. Hao, Y. L. Qiu, C.-W. School of Electrical and Electronic Engineering School of Materials Science & Engineering School of Mechanical and Aerospace Engineering School of Biological Sciences Lee Kong Chian School of Medicine (LKCMedicine) Centre for Biomimetic Sensor Science Single-bacteria-level Screening Particle Trapping and Binding Particle trapping and binding in optical potential wells provide a versatile platform for various biomedical applications. However, implementation systems to study multi-particle contact interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we demonstrate the precise control of particle interactions and functions such as controlling aggregation and multi-hopping. The mean residence time of a single particle is found considerably reduced from 7 s, as predicted by Kramer’s theory, to 0.6 s, owing to the mechanical interactions among aggregated particles. The optofluidic lattice also enables single-bacteria-level screening of biological binding agents such as antibodies through particle-enabled bacteria hopping. The binding efficiency of antibodies could be determined directly, selectively, quantitatively and efficiently. This work enriches the fundamental mechanisms of particle kinetics and offers new possibilities for probing and utilising unprecedented biomolecule interactions at single-bacteria level. NRF (Natl Research Foundation, S’pore) Published version 2018-08-02T06:15:02Z 2019-12-06T16:43:18Z 2018-08-02T06:15:02Z 2019-12-06T16:43:18Z 2018 Journal Article Shi, Y. Z., Xiong, S., Zhang, Y., Chin, L. K., Chen, Y.–Y., Zhang, J. B., et al. (2018). Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement. Nature Communications, 9(1), 815-. https://hdl.handle.net/10356/87504 http://hdl.handle.net/10220/45434 10.1038/s41467-018-03156-5 en Nature Communications © 2018 The Author(s) (Nature Publishing Group). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 11 p. application/pdf |
| spellingShingle | Single-bacteria-level Screening Particle Trapping and Binding Shi, Yu Zhi Xiong, Sha Zhang, Yi Chin, Lip Ket Chen, Yan-Yu Zhang, Jing Bo Ser, Wee Hoi, Lim Siau Liedberg, Bo Yap, Peng Huat Tsai, Din Ping Liu, Ai Qun Zhang, T. H. Larson, A. Wu, J. H. Chen, T. N. Yang, Z. C. Hao, Y. L. Qiu, C.-W. Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement |
| title | Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement |
| title_full | Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement |
| title_fullStr | Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement |
| title_full_unstemmed | Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement |
| title_short | Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement |
| title_sort | sculpting nanoparticle dynamics for single bacteria level screening and direct binding efficiency measurement |
| topic | Single-bacteria-level Screening Particle Trapping and Binding |
| url | https://hdl.handle.net/10356/87504 http://hdl.handle.net/10220/45434 |
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