Applying Macromolecular Crowding to 3D Bioprinting: Fabrication of 3D Hierarchical Porous Collagen-based Hydrogel Constructs
Native tissues and/or organs possess complex hierarchical porous structures that confer highly-specific cellular functions. Despite advances in fabrication processes, it is still very challenging to emulate the hierarchical porous collagen architecture found in most native tissues. Hence, the abilit...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2018
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| Online Access: | https://hdl.handle.net/10356/87552 http://hdl.handle.net/10220/44478 |
| _version_ | 1824454416407724032 |
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| author | Ng, Wei Long Goh, Min Hao Yeong, Wai Yee Naing, May Win |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Ng, Wei Long Goh, Min Hao Yeong, Wai Yee Naing, May Win |
| author_sort | Ng, Wei Long |
| collection | NTU |
| description | Native tissues and/or organs possess complex hierarchical porous structures that confer highly-specific cellular functions. Despite advances in fabrication processes, it is still very challenging to emulate the hierarchical porous collagen architecture found in most native tissues. Hence, the ability to recreate such hierarchical porous structures would result in biomimetic tissue-engineered constructs. Here, a single-step drop-on-demand (DOD) bioprinting strategy is proposed to fabricate hierarchical porous collagen-based hydrogels. Printable macromolecule-based bio-inks (polyvinylpyrrolidone, PVP) have been developed and printed in a DOD manner to manipulate the porosity within the multi-layered collagen-based hydrogels by altering the collagen fibrillogenesis process. The experimental results have indicated that hierarchical porous collagen structures could be achieved by controlling the number of macromolecule-based bio-ink droplets printed on each printed collagen layer. This facile single-step bioprinting process could be useful for the structural design of collagen-based hydrogels for various tissue engineering applications. |
| first_indexed | 2025-02-19T03:21:58Z |
| format | Journal Article |
| id | ntu-10356/87552 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:21:58Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/875522020-09-26T22:07:56Z Applying Macromolecular Crowding to 3D Bioprinting: Fabrication of 3D Hierarchical Porous Collagen-based Hydrogel Constructs Ng, Wei Long Goh, Min Hao Yeong, Wai Yee Naing, May Win School of Mechanical and Aerospace Engineering A*STAR SIMTech Singapore Centre for 3D Printing Macromolecular Crowding 3D Bioprinting Native tissues and/or organs possess complex hierarchical porous structures that confer highly-specific cellular functions. Despite advances in fabrication processes, it is still very challenging to emulate the hierarchical porous collagen architecture found in most native tissues. Hence, the ability to recreate such hierarchical porous structures would result in biomimetic tissue-engineered constructs. Here, a single-step drop-on-demand (DOD) bioprinting strategy is proposed to fabricate hierarchical porous collagen-based hydrogels. Printable macromolecule-based bio-inks (polyvinylpyrrolidone, PVP) have been developed and printed in a DOD manner to manipulate the porosity within the multi-layered collagen-based hydrogels by altering the collagen fibrillogenesis process. The experimental results have indicated that hierarchical porous collagen structures could be achieved by controlling the number of macromolecule-based bio-ink droplets printed on each printed collagen layer. This facile single-step bioprinting process could be useful for the structural design of collagen-based hydrogels for various tissue engineering applications. Accepted version 2018-03-02T02:34:26Z 2019-12-06T16:44:23Z 2018-03-02T02:34:26Z 2019-12-06T16:44:23Z 2018 Journal Article Ng, W. L., Goh, M. H., Yeong, W. Y., & Naing, M. W. (2018). Applying Macromolecular Crowding to 3D Bioprinting: Fabrication of 3D Hierarchical Porous Collagen-based Hydrogel Constructs. Biomaterials Science, 6(3), 562-574. 2047-4830 https://hdl.handle.net/10356/87552 http://hdl.handle.net/10220/44478 10.1039/C7BM01015J en Biomaterials Science © 2018 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Biomaterials Science, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1039/C7BM01015J]. 13 p. application/pdf |
| spellingShingle | Macromolecular Crowding 3D Bioprinting Ng, Wei Long Goh, Min Hao Yeong, Wai Yee Naing, May Win Applying Macromolecular Crowding to 3D Bioprinting: Fabrication of 3D Hierarchical Porous Collagen-based Hydrogel Constructs |
| title | Applying Macromolecular Crowding to 3D Bioprinting: Fabrication of 3D Hierarchical Porous Collagen-based Hydrogel Constructs |
| title_full | Applying Macromolecular Crowding to 3D Bioprinting: Fabrication of 3D Hierarchical Porous Collagen-based Hydrogel Constructs |
| title_fullStr | Applying Macromolecular Crowding to 3D Bioprinting: Fabrication of 3D Hierarchical Porous Collagen-based Hydrogel Constructs |
| title_full_unstemmed | Applying Macromolecular Crowding to 3D Bioprinting: Fabrication of 3D Hierarchical Porous Collagen-based Hydrogel Constructs |
| title_short | Applying Macromolecular Crowding to 3D Bioprinting: Fabrication of 3D Hierarchical Porous Collagen-based Hydrogel Constructs |
| title_sort | applying macromolecular crowding to 3d bioprinting fabrication of 3d hierarchical porous collagen based hydrogel constructs |
| topic | Macromolecular Crowding 3D Bioprinting |
| url | https://hdl.handle.net/10356/87552 http://hdl.handle.net/10220/44478 |
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