Rapid cell separation with minimal manipulation for autologous cell therapies
The ability to isolate specific, viable cell populations from mixed ensembles with minimal manipulation and within intra-operative time would provide significant advantages for autologous, cell-based therapies in regenerative medicine. Current cell-enrichment technologies are either slow, lack speci...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/87589 http://hdl.handle.net/10220/45460 |
| _version_ | 1811681961843884032 |
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| author | Smith, Alban J. O’Rorke, Richard D. Kale, Akshay Rimsa, Roberts Tomlinson, Matthew J. Kirkham, Jennifer Davies, A. Giles Wälti, Christoph Wood, Christopher D. |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Smith, Alban J. O’Rorke, Richard D. Kale, Akshay Rimsa, Roberts Tomlinson, Matthew J. Kirkham, Jennifer Davies, A. Giles Wälti, Christoph Wood, Christopher D. |
| author_sort | Smith, Alban J. |
| collection | NTU |
| description | The ability to isolate specific, viable cell populations from mixed ensembles with minimal manipulation and within intra-operative time would provide significant advantages for autologous, cell-based therapies in regenerative medicine. Current cell-enrichment technologies are either slow, lack specificity and/or require labelling. Thus a rapid, label-free separation technology that does not affect cell functionality, viability or phenotype is highly desirable. Here, we demonstrate separation of viable from non-viable human stromal cells using remote dielectrophoresis, in which an electric field is coupled into a microfluidic channel using shear-horizontal surface acoustic waves, producing an array of virtual electrodes within the channel. This allows high-throughput dielectrophoretic cell separation in high conductivity, physiological-like fluids, overcoming the limitations of conventional dielectrophoresis. We demonstrate viable/non-viable separation efficacy of >98% in pre-purified mesenchymal stromal cells, extracted from human dental pulp, with no adverse effects on cell viability, or on their subsequent osteogenic capabilities. |
| first_indexed | 2024-10-01T03:49:16Z |
| format | Journal Article |
| id | ntu-10356/87589 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:49:16Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/875892023-07-14T15:51:57Z Rapid cell separation with minimal manipulation for autologous cell therapies Smith, Alban J. O’Rorke, Richard D. Kale, Akshay Rimsa, Roberts Tomlinson, Matthew J. Kirkham, Jennifer Davies, A. Giles Wälti, Christoph Wood, Christopher D. School of Materials Science & Engineering Minimal Manipulation Autologous Cell Therapies The ability to isolate specific, viable cell populations from mixed ensembles with minimal manipulation and within intra-operative time would provide significant advantages for autologous, cell-based therapies in regenerative medicine. Current cell-enrichment technologies are either slow, lack specificity and/or require labelling. Thus a rapid, label-free separation technology that does not affect cell functionality, viability or phenotype is highly desirable. Here, we demonstrate separation of viable from non-viable human stromal cells using remote dielectrophoresis, in which an electric field is coupled into a microfluidic channel using shear-horizontal surface acoustic waves, producing an array of virtual electrodes within the channel. This allows high-throughput dielectrophoretic cell separation in high conductivity, physiological-like fluids, overcoming the limitations of conventional dielectrophoresis. We demonstrate viable/non-viable separation efficacy of >98% in pre-purified mesenchymal stromal cells, extracted from human dental pulp, with no adverse effects on cell viability, or on their subsequent osteogenic capabilities. Published version 2018-08-06T04:20:26Z 2019-12-06T16:45:08Z 2018-08-06T04:20:26Z 2019-12-06T16:45:08Z 2017 Journal Article Smith, A. J., O’Rorke, R. D., Kale, A., Rimsa, R., Tomlinson, M. J., Kirkham, J., et al. (2017). Rapid cell separation with minimal manipulation for autologous cell therapies. Scientific Reports, 7, 41872-. 2045-2322 https://hdl.handle.net/10356/87589 http://hdl.handle.net/10220/45460 10.1038/srep41872 en Scientific Reports © 2017 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 15 p. application/pdf |
| spellingShingle | Minimal Manipulation Autologous Cell Therapies Smith, Alban J. O’Rorke, Richard D. Kale, Akshay Rimsa, Roberts Tomlinson, Matthew J. Kirkham, Jennifer Davies, A. Giles Wälti, Christoph Wood, Christopher D. Rapid cell separation with minimal manipulation for autologous cell therapies |
| title | Rapid cell separation with minimal manipulation for autologous cell therapies |
| title_full | Rapid cell separation with minimal manipulation for autologous cell therapies |
| title_fullStr | Rapid cell separation with minimal manipulation for autologous cell therapies |
| title_full_unstemmed | Rapid cell separation with minimal manipulation for autologous cell therapies |
| title_short | Rapid cell separation with minimal manipulation for autologous cell therapies |
| title_sort | rapid cell separation with minimal manipulation for autologous cell therapies |
| topic | Minimal Manipulation Autologous Cell Therapies |
| url | https://hdl.handle.net/10356/87589 http://hdl.handle.net/10220/45460 |
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