A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting
ARPE‐19 and Y79 cells were precisely and effectively delivered to form an in vitro retinal tissue model via 3D cell bioprinting technology. The samples were characterized by cell viability assay, hematoxylin and eosin (HE) and immunofluorescent staining, scanning electrical microscopy (SEM) and conf...
| Päätekijät: | , , , , |
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| Muut tekijät: | |
| Aineistotyyppi: | Journal Article |
| Kieli: | English |
| Julkaistu: |
2018
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| Aiheet: | |
| Linkit: | https://hdl.handle.net/10356/87606 http://hdl.handle.net/10220/44606 |
| _version_ | 1826122973642227712 |
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| author | Shi, Pujiang Tan, Yong Sheng Edgar Yeong, Wai Yee Li, Hoi Yeung Laude, Augustinus |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Shi, Pujiang Tan, Yong Sheng Edgar Yeong, Wai Yee Li, Hoi Yeung Laude, Augustinus |
| author_sort | Shi, Pujiang |
| collection | NTU |
| description | ARPE‐19 and Y79 cells were precisely and effectively delivered to form an in vitro retinal tissue model via 3D cell bioprinting technology. The samples were characterized by cell viability assay, hematoxylin and eosin (HE) and immunofluorescent staining, scanning electrical microscopy (SEM) and confocal microscopy etc. The bioprinted ARPE‐19 cells formed a high‐quality cell monolayer in 14 days. Manually seeded ARPE‐19 cells were poorly controlled during and after cell seeding, and they aggregated to form uneven cell layer. The Y79 cells were subsequently bioprinted on the ARPE‐19 cell monolayer to form two distinctive patterns. The microvalve‐based bioprinting is efficient and accurate to build the in vitro tissue models with the potential to provide similar pathological responses and mechanism to human diseases, to mimic the phenotypic endpoints that are comparable to clinical studies, and to provide a realistic prediction of clinical efficacy. |
| first_indexed | 2024-10-01T05:57:21Z |
| format | Journal Article |
| id | ntu-10356/87606 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:57:21Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/876062020-09-26T22:06:49Z A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting Shi, Pujiang Tan, Yong Sheng Edgar Yeong, Wai Yee Li, Hoi Yeung Laude, Augustinus School of Mechanical and Aerospace Engineering School of Biological Sciences Singapore Centre for 3D Printing Bioprinting Retina ARPE‐19 and Y79 cells were precisely and effectively delivered to form an in vitro retinal tissue model via 3D cell bioprinting technology. The samples were characterized by cell viability assay, hematoxylin and eosin (HE) and immunofluorescent staining, scanning electrical microscopy (SEM) and confocal microscopy etc. The bioprinted ARPE‐19 cells formed a high‐quality cell monolayer in 14 days. Manually seeded ARPE‐19 cells were poorly controlled during and after cell seeding, and they aggregated to form uneven cell layer. The Y79 cells were subsequently bioprinted on the ARPE‐19 cell monolayer to form two distinctive patterns. The microvalve‐based bioprinting is efficient and accurate to build the in vitro tissue models with the potential to provide similar pathological responses and mechanism to human diseases, to mimic the phenotypic endpoints that are comparable to clinical studies, and to provide a realistic prediction of clinical efficacy. Accepted version 2018-03-23T07:18:08Z 2019-12-06T16:45:30Z 2018-03-23T07:18:08Z 2019-12-06T16:45:30Z 2018 2018 Journal Article Shi, P., Tan, Y. S. E., Yeong, W. Y., Li, H. Y., & Laude, A. (2018). A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting. Journal of Tissue Engineering and Regenerative Medicine, 12(5), 1297-1306. 1932-6254 https://hdl.handle.net/10356/87606 http://hdl.handle.net/10220/44606 10.1002/term.2661 203458 en Journal of Tissue Engineering and Regenerative Medicine © 2018 John Wiley & Sons Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/term.2661]. 24 p. application/pdf |
| spellingShingle | Bioprinting Retina Shi, Pujiang Tan, Yong Sheng Edgar Yeong, Wai Yee Li, Hoi Yeung Laude, Augustinus A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting |
| title | A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting |
| title_full | A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting |
| title_fullStr | A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting |
| title_full_unstemmed | A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting |
| title_short | A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting |
| title_sort | bilayer photoreceptor retinal tissue model with gradient cell density design a study of microvalve based bioprinting |
| topic | Bioprinting Retina |
| url | https://hdl.handle.net/10356/87606 http://hdl.handle.net/10220/44606 |
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