The balance between conventional DCs and plasmacytoid DCs is pivotal for immunological tolerance during pregnancy in the mouse
Dendritic cells (DCs), which can shape their functions depending on the microenvironment, are crucial for the delicate balance of immunity and tolerance during pregnancy. However, the mechanism underlying the microenvironment-educated plasticity of DC differentiation during pregnancy remains largely...
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/87628 http://hdl.handle.net/10220/46758 |
| _version_ | 1811696995010609152 |
|---|---|
| author | Shi, Meng Fang, Wen-Ning Meng, Chao-Yang Li, Dan-Dan Peng, Jing-Pian |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Shi, Meng Fang, Wen-Ning Meng, Chao-Yang Li, Dan-Dan Peng, Jing-Pian |
| author_sort | Shi, Meng |
| collection | NTU |
| description | Dendritic cells (DCs), which can shape their functions depending on the microenvironment, are crucial for the delicate balance of immunity and tolerance during pregnancy. However, the mechanism underlying the microenvironment-educated plasticity of DC differentiation during pregnancy remains largely unclear. Here, we found that the differentiation of conventional DCs (cDCs) and plasmacytoid DCs (pDCs) is regulated in a tissue-specific manner during pregnancy. The ratio of cDCs and pDCs remained constant in the spleen. However, the ratio changed in the para-aortic lymph nodes (LNs), where cDC percentages were significantly reduced concurrent with an increase in pDCs from E8.5 to E16.5. Moreover, the expansion of pDCs and T regulatory (Treg) cells was correlated in the para-aortic LNs, and pDCs had more potential to induce regulatory T cells (Tregs) compared with cDCs (independent of IDO expression). Notably, the balance between cDCs and pDCs is disrupted in IFN-γ-induced abnormal pregnancy, accompanied by lower Treg percentages in the para-aortic LNs and decidua. To further identify the underlying mechanism, we found that elevated IFN-γ can increase the levels of GM-CSF to alter the differentiation of pDCs into cDCs in vivo. Therefore, we provide a novel regulatory mechanism underlying pregnancy-related immune tolerance that involves the balance of DC subsets, which may offer a new target for the prevention of human spontaneous abortion. |
| first_indexed | 2024-10-01T07:48:13Z |
| format | Journal Article |
| id | ntu-10356/87628 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:48:13Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/876282023-02-28T16:56:17Z The balance between conventional DCs and plasmacytoid DCs is pivotal for immunological tolerance during pregnancy in the mouse Shi, Meng Fang, Wen-Ning Meng, Chao-Yang Li, Dan-Dan Peng, Jing-Pian School of Biological Sciences Immune Tolerance Dendritic Cells DRNTU::Science::Biological sciences Dendritic cells (DCs), which can shape their functions depending on the microenvironment, are crucial for the delicate balance of immunity and tolerance during pregnancy. However, the mechanism underlying the microenvironment-educated plasticity of DC differentiation during pregnancy remains largely unclear. Here, we found that the differentiation of conventional DCs (cDCs) and plasmacytoid DCs (pDCs) is regulated in a tissue-specific manner during pregnancy. The ratio of cDCs and pDCs remained constant in the spleen. However, the ratio changed in the para-aortic lymph nodes (LNs), where cDC percentages were significantly reduced concurrent with an increase in pDCs from E8.5 to E16.5. Moreover, the expansion of pDCs and T regulatory (Treg) cells was correlated in the para-aortic LNs, and pDCs had more potential to induce regulatory T cells (Tregs) compared with cDCs (independent of IDO expression). Notably, the balance between cDCs and pDCs is disrupted in IFN-γ-induced abnormal pregnancy, accompanied by lower Treg percentages in the para-aortic LNs and decidua. To further identify the underlying mechanism, we found that elevated IFN-γ can increase the levels of GM-CSF to alter the differentiation of pDCs into cDCs in vivo. Therefore, we provide a novel regulatory mechanism underlying pregnancy-related immune tolerance that involves the balance of DC subsets, which may offer a new target for the prevention of human spontaneous abortion. Published version 2018-11-30T07:44:36Z 2019-12-06T16:46:00Z 2018-11-30T07:44:36Z 2019-12-06T16:46:00Z 2016 Journal Article Fang, W.-N., Shi, M., Meng, C.-Y., Li, D.-D., & Peng, J.-P. (2016). The balance between conventional DCs and plasmacytoid DCs is pivotal for immunological tolerance during pregnancy in the mouse. Scientific Reports, 6, 26984-. doi:10.1038/srep26984 https://hdl.handle.net/10356/87628 http://hdl.handle.net/10220/46758 10.1038/srep26984 27229324 en Scientific Reports © 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 11 p. application/pdf |
| spellingShingle | Immune Tolerance Dendritic Cells DRNTU::Science::Biological sciences Shi, Meng Fang, Wen-Ning Meng, Chao-Yang Li, Dan-Dan Peng, Jing-Pian The balance between conventional DCs and plasmacytoid DCs is pivotal for immunological tolerance during pregnancy in the mouse |
| title | The balance between conventional DCs and plasmacytoid DCs is pivotal for immunological tolerance during pregnancy in the mouse |
| title_full | The balance between conventional DCs and plasmacytoid DCs is pivotal for immunological tolerance during pregnancy in the mouse |
| title_fullStr | The balance between conventional DCs and plasmacytoid DCs is pivotal for immunological tolerance during pregnancy in the mouse |
| title_full_unstemmed | The balance between conventional DCs and plasmacytoid DCs is pivotal for immunological tolerance during pregnancy in the mouse |
| title_short | The balance between conventional DCs and plasmacytoid DCs is pivotal for immunological tolerance during pregnancy in the mouse |
| title_sort | balance between conventional dcs and plasmacytoid dcs is pivotal for immunological tolerance during pregnancy in the mouse |
| topic | Immune Tolerance Dendritic Cells DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/87628 http://hdl.handle.net/10220/46758 |
| work_keys_str_mv | AT shimeng thebalancebetweenconventionaldcsandplasmacytoiddcsispivotalforimmunologicaltoleranceduringpregnancyinthemouse AT fangwenning thebalancebetweenconventionaldcsandplasmacytoiddcsispivotalforimmunologicaltoleranceduringpregnancyinthemouse AT mengchaoyang thebalancebetweenconventionaldcsandplasmacytoiddcsispivotalforimmunologicaltoleranceduringpregnancyinthemouse AT lidandan thebalancebetweenconventionaldcsandplasmacytoiddcsispivotalforimmunologicaltoleranceduringpregnancyinthemouse AT pengjingpian thebalancebetweenconventionaldcsandplasmacytoiddcsispivotalforimmunologicaltoleranceduringpregnancyinthemouse AT shimeng balancebetweenconventionaldcsandplasmacytoiddcsispivotalforimmunologicaltoleranceduringpregnancyinthemouse AT fangwenning balancebetweenconventionaldcsandplasmacytoiddcsispivotalforimmunologicaltoleranceduringpregnancyinthemouse AT mengchaoyang balancebetweenconventionaldcsandplasmacytoiddcsispivotalforimmunologicaltoleranceduringpregnancyinthemouse AT lidandan balancebetweenconventionaldcsandplasmacytoiddcsispivotalforimmunologicaltoleranceduringpregnancyinthemouse AT pengjingpian balancebetweenconventionaldcsandplasmacytoiddcsispivotalforimmunologicaltoleranceduringpregnancyinthemouse |