Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound
Objectives: Low‐intensity ultrasound (LIUS) has been shown to enhance bone and cartilage regeneration from stem cells. The ease of its incorporation makes it an attractive mechanical stimulus for not only osteogenesis and chondrogenesis, but also cardiomyogenesis. However, to date, no study has inve...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/87804 http://hdl.handle.net/10220/45527 |
| _version_ | 1811680838335594496 |
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| author | Teo, Ailing Morshedi, Amir Wang, Jen-Chieh Zhou, Yufeng Lim, Mayasari |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Teo, Ailing Morshedi, Amir Wang, Jen-Chieh Zhou, Yufeng Lim, Mayasari |
| author_sort | Teo, Ailing |
| collection | NTU |
| description | Objectives: Low‐intensity ultrasound (LIUS) has been shown to enhance bone and cartilage regeneration from stem cells. The ease of its incorporation makes it an attractive mechanical stimulus for not only osteogenesis and chondrogenesis, but also cardiomyogenesis. However, to date, no study has investigated its effects on cardiomyogenesis from embryonic stem cells. Methods: In this study, murine embryonic stem cells were differentiated via embryoid body formation and plating, and after 3 days they were subjected to daily 10 minutes of LIUS treatment with various conditions: (1) low‐pulsed (21 mW/cm2, 20% duty cycle), (2) low‐continuous, (3) high‐pulsed (147 mW/cm2, 20% duty cycle), and (4) high‐continuous LIUS. Results: Low‐pulsed and high‐continuous LIUS had improved beating rates of contractile areas as well as increased late cardiac gene expressions, such as α‐ and β‐myosin heavy chain and cardiac troponin T, showing its benefits on cardiomyocyte differentiation. Meanwhile, an early endodermal marker, α‐fetoprotein, was significantly attenuated after LIUS treatments. Conclusions: With these observations, it is demonstrated that LIUS simulation could enhance cardiomyogenesis from embryonic stem cells and increase its selectivity toward cardiomyocytes by reducing spontaneous differentiation. |
| first_indexed | 2024-10-01T03:31:25Z |
| format | Journal Article |
| id | ntu-10356/87804 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:31:25Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/878042023-12-29T06:46:39Z Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound Teo, Ailing Morshedi, Amir Wang, Jen-Chieh Zhou, Yufeng Lim, Mayasari School of Chemical and Biomedical Engineering School of Mechanical and Aerospace Engineering Embryonic Stem Cells Cardiomyogenesis Objectives: Low‐intensity ultrasound (LIUS) has been shown to enhance bone and cartilage regeneration from stem cells. The ease of its incorporation makes it an attractive mechanical stimulus for not only osteogenesis and chondrogenesis, but also cardiomyogenesis. However, to date, no study has investigated its effects on cardiomyogenesis from embryonic stem cells. Methods: In this study, murine embryonic stem cells were differentiated via embryoid body formation and plating, and after 3 days they were subjected to daily 10 minutes of LIUS treatment with various conditions: (1) low‐pulsed (21 mW/cm2, 20% duty cycle), (2) low‐continuous, (3) high‐pulsed (147 mW/cm2, 20% duty cycle), and (4) high‐continuous LIUS. Results: Low‐pulsed and high‐continuous LIUS had improved beating rates of contractile areas as well as increased late cardiac gene expressions, such as α‐ and β‐myosin heavy chain and cardiac troponin T, showing its benefits on cardiomyocyte differentiation. Meanwhile, an early endodermal marker, α‐fetoprotein, was significantly attenuated after LIUS treatments. Conclusions: With these observations, it is demonstrated that LIUS simulation could enhance cardiomyogenesis from embryonic stem cells and increase its selectivity toward cardiomyocytes by reducing spontaneous differentiation. Published version 2018-08-07T08:41:40Z 2019-12-06T16:49:49Z 2018-08-07T08:41:40Z 2019-12-06T16:49:49Z 2017 Journal Article Teo, A., Morshedi, A., Wang, J. C., Zhou, Y., & Lim, M. (2017). Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound. Journal of Ultrasound in Medicine, 36(8), 1693-1706. 0278-4297 https://hdl.handle.net/10356/87804 http://hdl.handle.net/10220/45527 10.7863/ultra.16.12042 en Journal of Ultrasound in Medicine © 2017 American Institute of Ultrasound in Medicine. This paper was published in Journal of Ultrasound in Medicine and is made available as an electronic reprint (preprint) with permission of American Institute of Ultrasound in Medicine. The published version is available at: [http://dx.doi.org/10.7863/ultra.16.12042]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 14 p. application/pdf |
| spellingShingle | Embryonic Stem Cells Cardiomyogenesis Teo, Ailing Morshedi, Amir Wang, Jen-Chieh Zhou, Yufeng Lim, Mayasari Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound |
| title | Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound |
| title_full | Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound |
| title_fullStr | Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound |
| title_full_unstemmed | Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound |
| title_short | Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound |
| title_sort | enhancement of cardiomyogenesis in murine stem cells by low intensity ultrasound |
| topic | Embryonic Stem Cells Cardiomyogenesis |
| url | https://hdl.handle.net/10356/87804 http://hdl.handle.net/10220/45527 |
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