Diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in Liangshui River of Beijing, China
Aerobic denitrification is a process reducing the nitrate into gaseous nitrogen forms in the presence of oxygen gas, which makes the nitrification and denitrification performed simultaneously. However, little was known on the diversity of the culturable aerobic denitrifying bacteria in the surface w...
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/87899 http://hdl.handle.net/10220/45580 |
| _version_ | 1811680431164096512 |
|---|---|
| author | Lv, Pengyi Luo, Jinxue Zhuang, Xuliang Zhang, Dongqing Huang, Zhanbin Bai, Zhihui |
| author2 | Advanced Environmental Biotechnology Centre (AEBC) |
| author_facet | Advanced Environmental Biotechnology Centre (AEBC) Lv, Pengyi Luo, Jinxue Zhuang, Xuliang Zhang, Dongqing Huang, Zhanbin Bai, Zhihui |
| author_sort | Lv, Pengyi |
| collection | NTU |
| description | Aerobic denitrification is a process reducing the nitrate into gaseous nitrogen forms in the presence of oxygen gas, which makes the nitrification and denitrification performed simultaneously. However, little was known on the diversity of the culturable aerobic denitrifying bacteria in the surface water system. In this study, 116 strains of aerobic denitrifying bacteria were isolated from the sediment, water and biofilm samples in Liangshui River of Beijing. These bacteria were classified into 14 genera based on the 16 S rDNA, such as Pseudomonas, Rheinheimera, and Gemmobacter. The Pseudomonas sp., represented by the Pseudomonas stutzeri, Pseudomonas mendocina and Pseudomonas putida, composed the major culturable aerobic denitrifiers of the river, followed by Ochrobactrum sp. and Rheinheimera sp. The PCA plot showed the unclassified Pseudomonas sp. and Rheinheimera pacifica preferred to inhabit in biofilm phase while one unclassified Ochrobactrum sp. and Pseudomonas resinovorans had higher abundance in the sediment. In the overlying water, the Pseudomonas stutzeri and Ochrobactrum rhizosphaerae were found to have higher abundance, indicating these aerobic denitrifiers had different habitat-preferable characteristics among the 3 phases of river system. The findings may help select the niche to isolate the aerobic denitrifiers and facilitate the bioaugmentation-based purification of the nitrate polluted surface water. |
| first_indexed | 2024-10-01T03:24:56Z |
| format | Journal Article |
| id | ntu-10356/87899 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:24:56Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/878992020-09-26T21:58:35Z Diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in Liangshui River of Beijing, China Lv, Pengyi Luo, Jinxue Zhuang, Xuliang Zhang, Dongqing Huang, Zhanbin Bai, Zhihui Advanced Environmental Biotechnology Centre (AEBC) Nanyang Environment and Water Research Institute Aerobic Denitrifying Bacteria Water Aerobic denitrification is a process reducing the nitrate into gaseous nitrogen forms in the presence of oxygen gas, which makes the nitrification and denitrification performed simultaneously. However, little was known on the diversity of the culturable aerobic denitrifying bacteria in the surface water system. In this study, 116 strains of aerobic denitrifying bacteria were isolated from the sediment, water and biofilm samples in Liangshui River of Beijing. These bacteria were classified into 14 genera based on the 16 S rDNA, such as Pseudomonas, Rheinheimera, and Gemmobacter. The Pseudomonas sp., represented by the Pseudomonas stutzeri, Pseudomonas mendocina and Pseudomonas putida, composed the major culturable aerobic denitrifiers of the river, followed by Ochrobactrum sp. and Rheinheimera sp. The PCA plot showed the unclassified Pseudomonas sp. and Rheinheimera pacifica preferred to inhabit in biofilm phase while one unclassified Ochrobactrum sp. and Pseudomonas resinovorans had higher abundance in the sediment. In the overlying water, the Pseudomonas stutzeri and Ochrobactrum rhizosphaerae were found to have higher abundance, indicating these aerobic denitrifiers had different habitat-preferable characteristics among the 3 phases of river system. The findings may help select the niche to isolate the aerobic denitrifiers and facilitate the bioaugmentation-based purification of the nitrate polluted surface water. Published version 2018-08-17T02:30:22Z 2019-12-06T16:51:42Z 2018-08-17T02:30:22Z 2019-12-06T16:51:42Z 2017 Journal Article Lv, P., Luo, J., Zhuang, X., Zhang, D., Huang, Z., & Bai, Z. (2017). Diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in Liangshui River of Beijing, China. Scientific Reports, 7, 10032-. 2045-2322 https://hdl.handle.net/10356/87899 http://hdl.handle.net/10220/45580 10.1038/s41598-017-09556-9 en Scientific Reports © 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 12 p. application/pdf |
| spellingShingle | Aerobic Denitrifying Bacteria Water Lv, Pengyi Luo, Jinxue Zhuang, Xuliang Zhang, Dongqing Huang, Zhanbin Bai, Zhihui Diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in Liangshui River of Beijing, China |
| title | Diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in Liangshui River of Beijing, China |
| title_full | Diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in Liangshui River of Beijing, China |
| title_fullStr | Diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in Liangshui River of Beijing, China |
| title_full_unstemmed | Diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in Liangshui River of Beijing, China |
| title_short | Diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in Liangshui River of Beijing, China |
| title_sort | diversity of culturable aerobic denitrifying bacteria in the sediment water and biofilms in liangshui river of beijing china |
| topic | Aerobic Denitrifying Bacteria Water |
| url | https://hdl.handle.net/10356/87899 http://hdl.handle.net/10220/45580 |
| work_keys_str_mv | AT lvpengyi diversityofculturableaerobicdenitrifyingbacteriainthesedimentwaterandbiofilmsinliangshuiriverofbeijingchina AT luojinxue diversityofculturableaerobicdenitrifyingbacteriainthesedimentwaterandbiofilmsinliangshuiriverofbeijingchina AT zhuangxuliang diversityofculturableaerobicdenitrifyingbacteriainthesedimentwaterandbiofilmsinliangshuiriverofbeijingchina AT zhangdongqing diversityofculturableaerobicdenitrifyingbacteriainthesedimentwaterandbiofilmsinliangshuiriverofbeijingchina AT huangzhanbin diversityofculturableaerobicdenitrifyingbacteriainthesedimentwaterandbiofilmsinliangshuiriverofbeijingchina AT baizhihui diversityofculturableaerobicdenitrifyingbacteriainthesedimentwaterandbiofilmsinliangshuiriverofbeijingchina |