High-resolution transport-of-intensity quantitative phase microscopy with annular illumination
For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent diffraction limit. Unfortunately, in a conventional microscope with...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/87905 http://hdl.handle.net/10220/45587 |
| _version_ | 1811679451786772480 |
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| author | Zuo, Chao Sun, Jiasong Li, Jiaji Zhang, Jialin Asundi, Anand Chen, Qian |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Zuo, Chao Sun, Jiasong Li, Jiaji Zhang, Jialin Asundi, Anand Chen, Qian |
| author_sort | Zuo, Chao |
| collection | NTU |
| description | For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent diffraction limit. Unfortunately, in a conventional microscope with circular illumination aperture, partial coherence tends to diminish the phase contrast, exacerbating the inherent noise-to-resolution tradeoff in TIE imaging, resulting in strong low-frequency artifacts and compromised imaging resolution. Here, we demonstrate how these issues can be effectively addressed by replacing the conventional circular illumination aperture with an annular one. The matched annular illumination not only strongly boosts the phase contrast for low spatial frequencies, but significantly improves the practical imaging resolution to near the incoherent diffraction limit. By incorporating high-numerical aperture (NA) illumination as well as high-NA objective, it is shown, for the first time, that TIE phase imaging can achieve a transverse resolution up to 208 nm, corresponding to an effective NA of 2.66. Time-lapse imaging of in vitro Hela cells revealing cellular morphology and subcellular dynamics during cells mitosis and apoptosis is exemplified. Given its capability for high-resolution QPI as well as the compatibility with widely available brightfield microscopy hardware, the proposed approach is expected to be adopted by the wider biology and medicine community. |
| first_indexed | 2024-10-01T03:09:22Z |
| format | Journal Article |
| id | ntu-10356/87905 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:09:22Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/879052023-03-04T17:18:37Z High-resolution transport-of-intensity quantitative phase microscopy with annular illumination Zuo, Chao Sun, Jiasong Li, Jiaji Zhang, Jialin Asundi, Anand Chen, Qian School of Mechanical and Aerospace Engineering Centre for Optical and Laser Engineering DRNTU::Engineering::Mechanical engineering Transport-of-intensity Equation Microscopy For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent diffraction limit. Unfortunately, in a conventional microscope with circular illumination aperture, partial coherence tends to diminish the phase contrast, exacerbating the inherent noise-to-resolution tradeoff in TIE imaging, resulting in strong low-frequency artifacts and compromised imaging resolution. Here, we demonstrate how these issues can be effectively addressed by replacing the conventional circular illumination aperture with an annular one. The matched annular illumination not only strongly boosts the phase contrast for low spatial frequencies, but significantly improves the practical imaging resolution to near the incoherent diffraction limit. By incorporating high-numerical aperture (NA) illumination as well as high-NA objective, it is shown, for the first time, that TIE phase imaging can achieve a transverse resolution up to 208 nm, corresponding to an effective NA of 2.66. Time-lapse imaging of in vitro Hela cells revealing cellular morphology and subcellular dynamics during cells mitosis and apoptosis is exemplified. Given its capability for high-resolution QPI as well as the compatibility with widely available brightfield microscopy hardware, the proposed approach is expected to be adopted by the wider biology and medicine community. Published version 2018-08-17T06:17:17Z 2019-12-06T16:51:51Z 2018-08-17T06:17:17Z 2019-12-06T16:51:51Z 2017 Journal Article Zuo, C., Sun, J., Li, J., Zhang, J., Asundi, A., & Chen, Q. (2017). High-resolution transport-of-intensity quantitative phase microscopy with annular illumination. Scientific Reports, 7(1), 7654-. 2045-2322 https://hdl.handle.net/10356/87905 http://hdl.handle.net/10220/45587 10.1038/s41598-017-06837-1 en Scientific Reports © 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 22 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Mechanical engineering Transport-of-intensity Equation Microscopy Zuo, Chao Sun, Jiasong Li, Jiaji Zhang, Jialin Asundi, Anand Chen, Qian High-resolution transport-of-intensity quantitative phase microscopy with annular illumination |
| title | High-resolution transport-of-intensity quantitative phase microscopy with annular illumination |
| title_full | High-resolution transport-of-intensity quantitative phase microscopy with annular illumination |
| title_fullStr | High-resolution transport-of-intensity quantitative phase microscopy with annular illumination |
| title_full_unstemmed | High-resolution transport-of-intensity quantitative phase microscopy with annular illumination |
| title_short | High-resolution transport-of-intensity quantitative phase microscopy with annular illumination |
| title_sort | high resolution transport of intensity quantitative phase microscopy with annular illumination |
| topic | DRNTU::Engineering::Mechanical engineering Transport-of-intensity Equation Microscopy |
| url | https://hdl.handle.net/10356/87905 http://hdl.handle.net/10220/45587 |
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