Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel)
Microbial methane oxidation is the primary control on the emission of the greenhouse gas methane into the atmosphere. In terrestrial environments, aerobic methanotrophic bacteria are largely responsible for this process. In marine sediments, a coupling of anaerobic oxidation of methane (AOM) with su...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/87910 http://hdl.handle.net/10220/46854 |
| _version_ | 1811689366082289664 |
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| author | Kushmaro, Ariel Eckert, W. Sivan, O. Bar-Or, I. Ben-Dov, E. |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Kushmaro, Ariel Eckert, W. Sivan, O. Bar-Or, I. Ben-Dov, E. |
| author_sort | Kushmaro, Ariel |
| collection | NTU |
| description | Microbial methane oxidation is the primary control on the emission of the greenhouse gas methane into the atmosphere. In terrestrial environments, aerobic methanotrophic bacteria are largely responsible for this process. In marine sediments, a coupling of anaerobic oxidation of methane (AOM) with sulfate reduction, often carried out by a consortium of anaerobic methanotrophic archaea (ANME) and sulfate-reducing bacteria, consumes almost all methane produced within those sediments. Motivated by recent evidence for AOM with iron(III) in Lake Kinneret sediments, the goal of the present study was to link the geochemical gradients in the lake porewater to the microbial community structure. Screening of archaeal 16S rRNA gene sequences revealed a shift from hydrogenotrophic to acetoclastic methanogens with depth. The observed changes in microbial community structure suggest possible direct and indirect mechanisms for the AOM coupled to iron reduction in deep sediments. The percentage of members of the Nitrospirales order increased with depth, suggesting their involvement in iron reduction together with Geobacter genus and "reverse methanogenesis". An indirect mechanism through sulfate and ANME seems less probable due to the absence of ANME sequences. This is despite the abundant sequences related to sulfate-reducing bacteria (Deltaproteobacteria) together with the occurrence of dsrA in the deep sediment that could indicate the production of sulfate (disproportionation) from S0 for sulfate-driven AOM. The presence of the functional gene pmoA in the deep anoxic sediment together with sequences related to Methylococcales suggests the existence of a second unexpected indirect pathway – aerobic methane oxidation pathway in an anaerobic environment. |
| first_indexed | 2024-10-01T05:46:57Z |
| format | Journal Article |
| id | ntu-10356/87910 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:46:57Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/879102023-07-14T15:45:53Z Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel) Kushmaro, Ariel Eckert, W. Sivan, O. Bar-Or, I. Ben-Dov, E. School of Materials Science & Engineering Lake Kinneret Anaerobic Oxidation of Methane DRNTU::Engineering::Materials Microbial methane oxidation is the primary control on the emission of the greenhouse gas methane into the atmosphere. In terrestrial environments, aerobic methanotrophic bacteria are largely responsible for this process. In marine sediments, a coupling of anaerobic oxidation of methane (AOM) with sulfate reduction, often carried out by a consortium of anaerobic methanotrophic archaea (ANME) and sulfate-reducing bacteria, consumes almost all methane produced within those sediments. Motivated by recent evidence for AOM with iron(III) in Lake Kinneret sediments, the goal of the present study was to link the geochemical gradients in the lake porewater to the microbial community structure. Screening of archaeal 16S rRNA gene sequences revealed a shift from hydrogenotrophic to acetoclastic methanogens with depth. The observed changes in microbial community structure suggest possible direct and indirect mechanisms for the AOM coupled to iron reduction in deep sediments. The percentage of members of the Nitrospirales order increased with depth, suggesting their involvement in iron reduction together with Geobacter genus and "reverse methanogenesis". An indirect mechanism through sulfate and ANME seems less probable due to the absence of ANME sequences. This is despite the abundant sequences related to sulfate-reducing bacteria (Deltaproteobacteria) together with the occurrence of dsrA in the deep sediment that could indicate the production of sulfate (disproportionation) from S0 for sulfate-driven AOM. The presence of the functional gene pmoA in the deep anoxic sediment together with sequences related to Methylococcales suggests the existence of a second unexpected indirect pathway – aerobic methane oxidation pathway in an anaerobic environment. Published version 2018-12-06T08:25:23Z 2019-12-06T16:51:56Z 2018-12-06T08:25:23Z 2019-12-06T16:51:56Z 2015 Journal Article Bar-Or, I., Ben-Dov, E., Kushmaro, A., Eckert, W., & Sivan, O. (2015). Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel). Biogeosciences, 12(10), 2847-2860. doi:10.5194/bg-12-2847-2015 1726-4170 https://hdl.handle.net/10356/87910 http://hdl.handle.net/10220/46854 10.5194/bg-12-2847-2015 en Biogeosciences © 2015 Author(s). CC Attribution 3.0 License. 14 p. application/pdf |
| spellingShingle | Lake Kinneret Anaerobic Oxidation of Methane DRNTU::Engineering::Materials Kushmaro, Ariel Eckert, W. Sivan, O. Bar-Or, I. Ben-Dov, E. Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel) |
| title | Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel) |
| title_full | Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel) |
| title_fullStr | Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel) |
| title_full_unstemmed | Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel) |
| title_short | Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel) |
| title_sort | methane related changes in prokaryotes along geochemical profiles in sediments of lake kinneret israel |
| topic | Lake Kinneret Anaerobic Oxidation of Methane DRNTU::Engineering::Materials |
| url | https://hdl.handle.net/10356/87910 http://hdl.handle.net/10220/46854 |
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