Microbial transcriptome profiling of black band disease in a Faviid coral during a seasonal disease peak
The etiology of black band disease (BBD), a persistent, globally distributed coral disease characterized by a dark microbial mat, is still unclear. A metatranscriptomics approach was used to unravel the roles of the major mat constituents in the disease process. By comparing the transcriptomes of th...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/87963 http://hdl.handle.net/10220/45575 |
| _version_ | 1826117248727646208 |
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| author | Arotsker, Luba Kramarsky-Winter, Esti Ben-Dov, Eitan Kushmaro, Ariel |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Arotsker, Luba Kramarsky-Winter, Esti Ben-Dov, Eitan Kushmaro, Ariel |
| author_sort | Arotsker, Luba |
| collection | NTU |
| description | The etiology of black band disease (BBD), a persistent, globally distributed coral disease characterized by a dark microbial mat, is still unclear. A metatranscriptomics approach was used to unravel the roles of the major mat constituents in the disease process. By comparing the transcriptomes of the mat constituents with those of the surface microbiota of diseased and healthy corals, we showed a shift in bacterial composition and function in BBD-affected corals. mRNA reads of Cyanobacteria, Bacteroidetes and Firmicutes phyla were prominent in the BBD mat. Cyanobacterial adenosylhomocysteinase, involved in cyanotoxin production, was the most transcribed gene in the band consortium. Pathogenic and non-pathogenic forms of Vibrio spp., mainly transcribing the thiamine ABC transporter, were abundant and highly active in both the band and surface tissues. Desulfovibrio desulfuricans was the primary producer of sulfide in the band. Members of the Bacilli class expressed high levels of rhodanese, an enzyme responsible for cyanide and sulfide detoxification. These results offer a first look at the varied functions of the microbiota in the disease mat and surrounding coral surface and enabled us to develop an improved functional model for this disease. |
| first_indexed | 2024-10-01T04:24:30Z |
| format | Journal Article |
| id | ntu-10356/87963 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:24:30Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/879632023-07-14T15:49:19Z Microbial transcriptome profiling of black band disease in a Faviid coral during a seasonal disease peak Arotsker, Luba Kramarsky-Winter, Esti Ben-Dov, Eitan Kushmaro, Ariel School of Materials Science & Engineering Coral Black Band Disease DRNTU::Engineering::Materials The etiology of black band disease (BBD), a persistent, globally distributed coral disease characterized by a dark microbial mat, is still unclear. A metatranscriptomics approach was used to unravel the roles of the major mat constituents in the disease process. By comparing the transcriptomes of the mat constituents with those of the surface microbiota of diseased and healthy corals, we showed a shift in bacterial composition and function in BBD-affected corals. mRNA reads of Cyanobacteria, Bacteroidetes and Firmicutes phyla were prominent in the BBD mat. Cyanobacterial adenosylhomocysteinase, involved in cyanotoxin production, was the most transcribed gene in the band consortium. Pathogenic and non-pathogenic forms of Vibrio spp., mainly transcribing the thiamine ABC transporter, were abundant and highly active in both the band and surface tissues. Desulfovibrio desulfuricans was the primary producer of sulfide in the band. Members of the Bacilli class expressed high levels of rhodanese, an enzyme responsible for cyanide and sulfide detoxification. These results offer a first look at the varied functions of the microbiota in the disease mat and surrounding coral surface and enabled us to develop an improved functional model for this disease. Published version 2018-08-16T09:13:34Z 2019-12-06T16:53:03Z 2018-08-16T09:13:34Z 2019-12-06T16:53:03Z 2016 Journal Article Arotsker, L., Kramarsky-Winter, E., Ben-Dov, E., & Kushmaro, A. (2016). Microbial transcriptome profiling of black band disease in a Faviid coral during a seasonal disease peak. Diseases of Aquatic Organisms, 118(1), 77-89. 0177-5103 https://hdl.handle.net/10356/87963 http://hdl.handle.net/10220/45575 10.3354/dao02952 en Diseases of Aquatic Organisms © 2016 Inter-Research. This paper was published in Diseases of Aquatic Organisms and is made available as an electronic reprint (preprint) with permission of Inter-Research. The published version is available at: [http://dx.doi.org/10.3354/dao02952]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 13 p. application/pdf |
| spellingShingle | Coral Black Band Disease DRNTU::Engineering::Materials Arotsker, Luba Kramarsky-Winter, Esti Ben-Dov, Eitan Kushmaro, Ariel Microbial transcriptome profiling of black band disease in a Faviid coral during a seasonal disease peak |
| title | Microbial transcriptome profiling of black band disease in a Faviid coral during a seasonal disease peak |
| title_full | Microbial transcriptome profiling of black band disease in a Faviid coral during a seasonal disease peak |
| title_fullStr | Microbial transcriptome profiling of black band disease in a Faviid coral during a seasonal disease peak |
| title_full_unstemmed | Microbial transcriptome profiling of black band disease in a Faviid coral during a seasonal disease peak |
| title_short | Microbial transcriptome profiling of black band disease in a Faviid coral during a seasonal disease peak |
| title_sort | microbial transcriptome profiling of black band disease in a faviid coral during a seasonal disease peak |
| topic | Coral Black Band Disease DRNTU::Engineering::Materials |
| url | https://hdl.handle.net/10356/87963 http://hdl.handle.net/10220/45575 |
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