Characterization of a thalamic nucleus mediating habenula responses to changes in ambient illumination
Background: Neural activity in the vertebrate habenula is affected by ambient illumination. The nucleus that links photoreceptor activity with the habenula is not well characterized. Here, we describe the location, inputs and potential function of this nucleus in larval zebrafish. Results: High-spee...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/87985 http://hdl.handle.net/10220/44534 |
| _version_ | 1824455791163211776 |
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| author | Cheng, Ruey-Kuang Krishnan, Seetha Lin, Qian Kibat, Caroline Jesuthasan, Suresh |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Cheng, Ruey-Kuang Krishnan, Seetha Lin, Qian Kibat, Caroline Jesuthasan, Suresh |
| author_sort | Cheng, Ruey-Kuang |
| collection | NTU |
| description | Background: Neural activity in the vertebrate habenula is affected by ambient illumination. The nucleus that links photoreceptor activity with the habenula is not well characterized. Here, we describe the location, inputs and potential function of this nucleus in larval zebrafish. Results: High-speed calcium imaging shows that light ON and OFF both evoke a rapid response in the dorsal left neuropil of the habenula, indicating preferential targeting of this neuropil by afferents conveying information about ambient illumination. Injection of a lipophilic dye into this neuropil led to bilateral labeling of a nucleus in the anterior thalamus that responds to light ON and OFF, and that receives innervation from the retina and pineal organ. Lesioning the neuropil of this thalamic nucleus reduced the habenula response to light ON and OFF. Optogenetic stimulation of the thalamus with channelrhodopsin-2 caused depolarization in the habenula, while manipulation with anion channelrhodopsins inhibited habenula response to light and disrupted climbing and diving evoked by illumination change. Conclusions: A nucleus in the anterior thalamus of larval zebrafish innervates the dorsal left habenula. This nucleus receives input from the retina and pineal, responds to increase and decrease in ambient illumination, enables habenula responses to change in irradiance, and may function in light-evoked vertical migration. |
| first_indexed | 2025-02-19T03:43:49Z |
| format | Journal Article |
| id | ntu-10356/87985 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:43:49Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/879852020-11-01T05:31:40Z Characterization of a thalamic nucleus mediating habenula responses to changes in ambient illumination Cheng, Ruey-Kuang Krishnan, Seetha Lin, Qian Kibat, Caroline Jesuthasan, Suresh Lee Kong Chian School of Medicine (LKCMedicine) Zebrafish Thalamus Background: Neural activity in the vertebrate habenula is affected by ambient illumination. The nucleus that links photoreceptor activity with the habenula is not well characterized. Here, we describe the location, inputs and potential function of this nucleus in larval zebrafish. Results: High-speed calcium imaging shows that light ON and OFF both evoke a rapid response in the dorsal left neuropil of the habenula, indicating preferential targeting of this neuropil by afferents conveying information about ambient illumination. Injection of a lipophilic dye into this neuropil led to bilateral labeling of a nucleus in the anterior thalamus that responds to light ON and OFF, and that receives innervation from the retina and pineal organ. Lesioning the neuropil of this thalamic nucleus reduced the habenula response to light ON and OFF. Optogenetic stimulation of the thalamus with channelrhodopsin-2 caused depolarization in the habenula, while manipulation with anion channelrhodopsins inhibited habenula response to light and disrupted climbing and diving evoked by illumination change. Conclusions: A nucleus in the anterior thalamus of larval zebrafish innervates the dorsal left habenula. This nucleus receives input from the retina and pineal, responds to increase and decrease in ambient illumination, enables habenula responses to change in irradiance, and may function in light-evoked vertical migration. Published version 2018-03-09T05:14:14Z 2019-12-06T16:53:32Z 2018-03-09T05:14:14Z 2019-12-06T16:53:32Z 2017 Journal Article Cheng, R.-K., Krishnan, S., Lin, Q., Kibat, C., & Jesuthasan, S. (2017). Characterization of a thalamic nucleus mediating habenula responses to changes in ambient illumination. BMC Biology, 15(1), 104-. https://hdl.handle.net/10356/87985 http://hdl.handle.net/10220/44534 10.1186/s12915-017-0431-1 en BMC Biology © 2017 Jesuthasan et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. 21 p. application/pdf |
| spellingShingle | Zebrafish Thalamus Cheng, Ruey-Kuang Krishnan, Seetha Lin, Qian Kibat, Caroline Jesuthasan, Suresh Characterization of a thalamic nucleus mediating habenula responses to changes in ambient illumination |
| title | Characterization of a thalamic nucleus mediating habenula responses to changes in ambient illumination |
| title_full | Characterization of a thalamic nucleus mediating habenula responses to changes in ambient illumination |
| title_fullStr | Characterization of a thalamic nucleus mediating habenula responses to changes in ambient illumination |
| title_full_unstemmed | Characterization of a thalamic nucleus mediating habenula responses to changes in ambient illumination |
| title_short | Characterization of a thalamic nucleus mediating habenula responses to changes in ambient illumination |
| title_sort | characterization of a thalamic nucleus mediating habenula responses to changes in ambient illumination |
| topic | Zebrafish Thalamus |
| url | https://hdl.handle.net/10356/87985 http://hdl.handle.net/10220/44534 |
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