The microbiomes of blowflies and houseflies as bacterial transmission reservoirs
Blowflies and houseflies are mechanical vectors inhabiting synanthropic environments around the world. They feed and breed in fecal and decaying organic matter, but the microbiome they harbour and transport is largely uncharacterized. We sampled 116 individual houseflies and blowflies from varying h...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/88233 http://hdl.handle.net/10220/45727 |
| _version_ | 1811685023506497536 |
|---|---|
| author | Junqueira, Ana Carolina Martins Ratan, Aakrosh Acerbi, Enzo Drautz-Moses, Daniela I. Premkrishnan, Balakrishnan N. V. Costea, Paul I. Linz, Bodo Purbojati, Rikky Wenang Paulo, Daniel F. Gaultier, Nicolas E. Subramanian, Poorani Hasan, Nur A. Colwell, Rita R. Bork, Peer Azeredo-Espin, Ana Maria L. Bryant, Donald A. Schuster, Stephan C. |
| author2 | Singapore Centre for Environmental Life Sciences Engineering |
| author_facet | Singapore Centre for Environmental Life Sciences Engineering Junqueira, Ana Carolina Martins Ratan, Aakrosh Acerbi, Enzo Drautz-Moses, Daniela I. Premkrishnan, Balakrishnan N. V. Costea, Paul I. Linz, Bodo Purbojati, Rikky Wenang Paulo, Daniel F. Gaultier, Nicolas E. Subramanian, Poorani Hasan, Nur A. Colwell, Rita R. Bork, Peer Azeredo-Espin, Ana Maria L. Bryant, Donald A. Schuster, Stephan C. |
| author_sort | Junqueira, Ana Carolina Martins |
| collection | NTU |
| description | Blowflies and houseflies are mechanical vectors inhabiting synanthropic environments around the world. They feed and breed in fecal and decaying organic matter, but the microbiome they harbour and transport is largely uncharacterized. We sampled 116 individual houseflies and blowflies from varying habitats on three continents and subjected them to high-coverage, whole-genome shotgun sequencing. This allowed for genomic and metagenomic analyses of the host-associated microbiome at the species level. Both fly host species segregate based on principal coordinate analysis of their microbial communities, but they also show an overlapping core microbiome. Legs and wings displayed the largest microbial diversity and were shown to be an important route for microbial dispersion. The environmental sequencing approach presented here detected a stochastic distribution of human pathogens, such as Helicobacter pylori, thereby demonstrating the potential of flies as proxies for environmental and public health surveillance. |
| first_indexed | 2024-10-01T04:37:56Z |
| format | Journal Article |
| id | ntu-10356/88233 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:37:56Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/882332020-09-21T11:33:21Z The microbiomes of blowflies and houseflies as bacterial transmission reservoirs Junqueira, Ana Carolina Martins Ratan, Aakrosh Acerbi, Enzo Drautz-Moses, Daniela I. Premkrishnan, Balakrishnan N. V. Costea, Paul I. Linz, Bodo Purbojati, Rikky Wenang Paulo, Daniel F. Gaultier, Nicolas E. Subramanian, Poorani Hasan, Nur A. Colwell, Rita R. Bork, Peer Azeredo-Espin, Ana Maria L. Bryant, Donald A. Schuster, Stephan C. Singapore Centre for Environmental Life Sciences Engineering Bacterial Transmission Microbial Community DRNTU::Science::Biological sciences Blowflies and houseflies are mechanical vectors inhabiting synanthropic environments around the world. They feed and breed in fecal and decaying organic matter, but the microbiome they harbour and transport is largely uncharacterized. We sampled 116 individual houseflies and blowflies from varying habitats on three continents and subjected them to high-coverage, whole-genome shotgun sequencing. This allowed for genomic and metagenomic analyses of the host-associated microbiome at the species level. Both fly host species segregate based on principal coordinate analysis of their microbial communities, but they also show an overlapping core microbiome. Legs and wings displayed the largest microbial diversity and were shown to be an important route for microbial dispersion. The environmental sequencing approach presented here detected a stochastic distribution of human pathogens, such as Helicobacter pylori, thereby demonstrating the potential of flies as proxies for environmental and public health surveillance. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Published version 2018-08-29T06:22:20Z 2019-12-06T16:58:46Z 2018-08-29T06:22:20Z 2019-12-06T16:58:46Z 2017 Journal Article Junqueira, A. C. M., Ratan, A., Acerbi, E., Drautz-Moses, D. I., Premkrishnan, B. N. V., Costea, P. I., ...Schuster, S. C. (2017). The microbiomes of blowflies and houseflies as bacterial transmission reservoirs. Scientific Reports, 7(1), 16324-. doi: 10.1038/s41598-017-16353-x. 2045-2322 https://hdl.handle.net/10356/88233 http://hdl.handle.net/10220/45727 10.1038/s41598-017-16353-x en Scientific Reports © 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 15 p. application/pdf |
| spellingShingle | Bacterial Transmission Microbial Community DRNTU::Science::Biological sciences Junqueira, Ana Carolina Martins Ratan, Aakrosh Acerbi, Enzo Drautz-Moses, Daniela I. Premkrishnan, Balakrishnan N. V. Costea, Paul I. Linz, Bodo Purbojati, Rikky Wenang Paulo, Daniel F. Gaultier, Nicolas E. Subramanian, Poorani Hasan, Nur A. Colwell, Rita R. Bork, Peer Azeredo-Espin, Ana Maria L. Bryant, Donald A. Schuster, Stephan C. The microbiomes of blowflies and houseflies as bacterial transmission reservoirs |
| title | The microbiomes of blowflies and houseflies as bacterial transmission reservoirs |
| title_full | The microbiomes of blowflies and houseflies as bacterial transmission reservoirs |
| title_fullStr | The microbiomes of blowflies and houseflies as bacterial transmission reservoirs |
| title_full_unstemmed | The microbiomes of blowflies and houseflies as bacterial transmission reservoirs |
| title_short | The microbiomes of blowflies and houseflies as bacterial transmission reservoirs |
| title_sort | microbiomes of blowflies and houseflies as bacterial transmission reservoirs |
| topic | Bacterial Transmission Microbial Community DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/88233 http://hdl.handle.net/10220/45727 |
| work_keys_str_mv | AT junqueiraanacarolinamartins themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT ratanaakrosh themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT acerbienzo themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT drautzmosesdanielai themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT premkrishnanbalakrishnannv themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT costeapauli themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT linzbodo themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT purbojatirikkywenang themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT paulodanielf themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT gaultiernicolase themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT subramanianpoorani themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT hasannura themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT colwellritar themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT borkpeer themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT azeredoespinanamarial themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT bryantdonalda themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT schusterstephanc themicrobiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT junqueiraanacarolinamartins microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT ratanaakrosh microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT acerbienzo microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT drautzmosesdanielai microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT premkrishnanbalakrishnannv microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT costeapauli microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT linzbodo microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT purbojatirikkywenang microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT paulodanielf microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT gaultiernicolase microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT subramanianpoorani microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT hasannura microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT colwellritar microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT borkpeer microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT azeredoespinanamarial microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT bryantdonalda microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs AT schusterstephanc microbiomesofblowfliesandhousefliesasbacterialtransmissionreservoirs |