Skin dendritic cell and T cell activation associated with dengue shock syndrome
The pathogenesis of severe dengue remains unclear, particularly the mechanisms underlying the plasma leakage that results in hypovolaemic shock in a small proportion of individuals. Maximal leakage occurs several days after peak viraemia implicating immunological pathways. Skin is a highly vascular...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2018
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| Online Access: | https://hdl.handle.net/10356/88278 http://hdl.handle.net/10220/45672 |
| _version_ | 1824456814602747904 |
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| author | Duyen, Huynh Thi Le Cerny, Daniela Trung, Dinh The Pang, Jassia Velumani, Sumathy Toh, Ying Xiu Qui, Phan Tu Hao, Nguyen Van Simmons, Cameron Haniffa, Muzlifah Wills, Bridget Fink, Katja |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Duyen, Huynh Thi Le Cerny, Daniela Trung, Dinh The Pang, Jassia Velumani, Sumathy Toh, Ying Xiu Qui, Phan Tu Hao, Nguyen Van Simmons, Cameron Haniffa, Muzlifah Wills, Bridget Fink, Katja |
| author_sort | Duyen, Huynh Thi Le |
| collection | NTU |
| description | The pathogenesis of severe dengue remains unclear, particularly the mechanisms underlying the plasma leakage that results in hypovolaemic shock in a small proportion of individuals. Maximal leakage occurs several days after peak viraemia implicating immunological pathways. Skin is a highly vascular organ and also an important site of immune reactions with a high density of dendritic cells (DCs), macrophages and T cells. We obtained skin biopsies and contemporaneous blood samples from patients within 24 hours of onset of dengue shock syndrome (DSS), and from healthy controls. We analyzed cell subsets by flow cytometry, and soluble mediators and antibodies by ELISA; the percentage of migratory CD1a+ dermal DCs was significantly decreased in the DSS patients, and skin CD8+ T cells were activated, but there was no accumulation of dengue-specific antibodies. Inflammatory monocytic cells were not observed infiltrating the skin of DSS cases on whole-mount histology, although CD14dim cells disappeared from blood. |
| first_indexed | 2025-02-19T04:00:05Z |
| format | Journal Article |
| id | ntu-10356/88278 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T04:00:05Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/882782023-02-28T17:02:19Z Skin dendritic cell and T cell activation associated with dengue shock syndrome Duyen, Huynh Thi Le Cerny, Daniela Trung, Dinh The Pang, Jassia Velumani, Sumathy Toh, Ying Xiu Qui, Phan Tu Hao, Nguyen Van Simmons, Cameron Haniffa, Muzlifah Wills, Bridget Fink, Katja School of Biological Sciences Skin Dengue Shock Syndrome DRNTU::Science::Biological sciences The pathogenesis of severe dengue remains unclear, particularly the mechanisms underlying the plasma leakage that results in hypovolaemic shock in a small proportion of individuals. Maximal leakage occurs several days after peak viraemia implicating immunological pathways. Skin is a highly vascular organ and also an important site of immune reactions with a high density of dendritic cells (DCs), macrophages and T cells. We obtained skin biopsies and contemporaneous blood samples from patients within 24 hours of onset of dengue shock syndrome (DSS), and from healthy controls. We analyzed cell subsets by flow cytometry, and soluble mediators and antibodies by ELISA; the percentage of migratory CD1a+ dermal DCs was significantly decreased in the DSS patients, and skin CD8+ T cells were activated, but there was no accumulation of dengue-specific antibodies. Inflammatory monocytic cells were not observed infiltrating the skin of DSS cases on whole-mount histology, although CD14dim cells disappeared from blood. Published version 2018-08-27T03:09:08Z 2019-12-06T16:59:43Z 2018-08-27T03:09:08Z 2019-12-06T16:59:43Z 2017 Journal Article Duyen, H. T. L., Cerny, D., Trung, D. T., Pang, J., Velumani, S., Toh, Y. X., . . . Fink, K. (2017). Skin dendritic cell and T cell activation associated with dengue shock syndrome. Scientific Reports, 7, 14224-. doi:10.1038/s41598-017-14640-1 2045-2322 https://hdl.handle.net/10356/88278 http://hdl.handle.net/10220/45672 10.1038/s41598-017-14640-1 en Scientific Reports © 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 12 p. application/pdf |
| spellingShingle | Skin Dengue Shock Syndrome DRNTU::Science::Biological sciences Duyen, Huynh Thi Le Cerny, Daniela Trung, Dinh The Pang, Jassia Velumani, Sumathy Toh, Ying Xiu Qui, Phan Tu Hao, Nguyen Van Simmons, Cameron Haniffa, Muzlifah Wills, Bridget Fink, Katja Skin dendritic cell and T cell activation associated with dengue shock syndrome |
| title | Skin dendritic cell and T cell activation associated with dengue shock syndrome |
| title_full | Skin dendritic cell and T cell activation associated with dengue shock syndrome |
| title_fullStr | Skin dendritic cell and T cell activation associated with dengue shock syndrome |
| title_full_unstemmed | Skin dendritic cell and T cell activation associated with dengue shock syndrome |
| title_short | Skin dendritic cell and T cell activation associated with dengue shock syndrome |
| title_sort | skin dendritic cell and t cell activation associated with dengue shock syndrome |
| topic | Skin Dengue Shock Syndrome DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/88278 http://hdl.handle.net/10220/45672 |
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