Single-cell transcriptomics of East-Asian pancreatic islets cells
Single-cell RNA-seq (scRNA-seq) of pancreatic islets have reported on α- and β-cell gene expression in mice and subjects of predominantly European ancestry. We aimed to assess these findings in East-Asian islet-cells. 448 islet-cells were captured from three East-Asian non-diabetic subjects for scRN...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/88280 http://hdl.handle.net/10220/45671 |
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| author | Tay, Vanessa Shi Yun Boehm, Bernhard Otto Dorajoo, Rajkumar Ali, Yusuf Kang, Jonathan Samydurai, Sudhagar Liu, Jianjun |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Tay, Vanessa Shi Yun Boehm, Bernhard Otto Dorajoo, Rajkumar Ali, Yusuf Kang, Jonathan Samydurai, Sudhagar Liu, Jianjun |
| author_sort | Tay, Vanessa Shi Yun |
| collection | NTU |
| description | Single-cell RNA-seq (scRNA-seq) of pancreatic islets have reported on α- and β-cell gene expression in mice and subjects of predominantly European ancestry. We aimed to assess these findings in East-Asian islet-cells. 448 islet-cells were captured from three East-Asian non-diabetic subjects for scRNA-seq. Hierarchical clustering using pancreatic cell lineage genes was used to assign cells into cell-types. Differentially expressed transcripts between α- and β-cells were detected using ANOVA and in silico replications of mouse and human islet cell genes were performed. We identified 118 α, 105 β, 6 δ endocrine cells and 47 exocrine cells. Besides INS and GCG, 26 genes showed differential expression between α- and β-cells. 10 genes showed concordant expression as reported in rodents, while FAM46A was significantly discordant. Comparing our East-Asian data with data from primarily European subjects, we replicated several genes implicated in nuclear receptor activations, acute phase response pathway, glutaryl-CoA/tryptophan degradations and EIF2/AMPK/mTOR signaling. Additionally, we identified protein ubiquitination to be associated among East-Asian β-cells. We report on East-Asian α- and β-cell gene signatures and substantiate several genes/pathways. We identify expression signatures in East-Asian β-cells that perhaps reflects increased susceptibility to cell-death and warrants future validations to fully appreciate their role in East-Asian diabetes pathogenesis. |
| first_indexed | 2024-10-01T06:21:40Z |
| format | Journal Article |
| id | ntu-10356/88280 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:21:40Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/882802020-11-01T05:23:29Z Single-cell transcriptomics of East-Asian pancreatic islets cells Tay, Vanessa Shi Yun Boehm, Bernhard Otto Dorajoo, Rajkumar Ali, Yusuf Kang, Jonathan Samydurai, Sudhagar Liu, Jianjun Lee Kong Chian School of Medicine (LKCMedicine) Gene Expression Islet Cell DRNTU::Science::Biological sciences Single-cell RNA-seq (scRNA-seq) of pancreatic islets have reported on α- and β-cell gene expression in mice and subjects of predominantly European ancestry. We aimed to assess these findings in East-Asian islet-cells. 448 islet-cells were captured from three East-Asian non-diabetic subjects for scRNA-seq. Hierarchical clustering using pancreatic cell lineage genes was used to assign cells into cell-types. Differentially expressed transcripts between α- and β-cells were detected using ANOVA and in silico replications of mouse and human islet cell genes were performed. We identified 118 α, 105 β, 6 δ endocrine cells and 47 exocrine cells. Besides INS and GCG, 26 genes showed differential expression between α- and β-cells. 10 genes showed concordant expression as reported in rodents, while FAM46A was significantly discordant. Comparing our East-Asian data with data from primarily European subjects, we replicated several genes implicated in nuclear receptor activations, acute phase response pathway, glutaryl-CoA/tryptophan degradations and EIF2/AMPK/mTOR signaling. Additionally, we identified protein ubiquitination to be associated among East-Asian β-cells. We report on East-Asian α- and β-cell gene signatures and substantiate several genes/pathways. We identify expression signatures in East-Asian β-cells that perhaps reflects increased susceptibility to cell-death and warrants future validations to fully appreciate their role in East-Asian diabetes pathogenesis. MOE (Min. of Education, S’pore) Published version 2018-08-27T02:29:42Z 2019-12-06T16:59:45Z 2018-08-27T02:29:42Z 2019-12-06T16:59:45Z 2017 Journal Article Dorajoo, R., Ali, Y., Tay, V. S. Y., Kang, J., Samydurai, S., Liu, J., & Boehm, B. O. (2017). Single-cell transcriptomics of East-Asian pancreatic islets cells. Scientific Reports, 7, 5024-. doi:10.1038/s41598-017-05266-4 2045-2322 https://hdl.handle.net/10356/88280 http://hdl.handle.net/10220/45671 10.1038/s41598-017-05266-4 en Scientific Reports © 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 8 p. application/pdf |
| spellingShingle | Gene Expression Islet Cell DRNTU::Science::Biological sciences Tay, Vanessa Shi Yun Boehm, Bernhard Otto Dorajoo, Rajkumar Ali, Yusuf Kang, Jonathan Samydurai, Sudhagar Liu, Jianjun Single-cell transcriptomics of East-Asian pancreatic islets cells |
| title | Single-cell transcriptomics of East-Asian pancreatic islets cells |
| title_full | Single-cell transcriptomics of East-Asian pancreatic islets cells |
| title_fullStr | Single-cell transcriptomics of East-Asian pancreatic islets cells |
| title_full_unstemmed | Single-cell transcriptomics of East-Asian pancreatic islets cells |
| title_short | Single-cell transcriptomics of East-Asian pancreatic islets cells |
| title_sort | single cell transcriptomics of east asian pancreatic islets cells |
| topic | Gene Expression Islet Cell DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/88280 http://hdl.handle.net/10220/45671 |
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