Cloning and characterization of EgGDSL, a gene associated with oil content in oil palm
Oil palm (Elaeis guineensis, Jacq.) is a key tropical oil crop, which provides over one third of the global vegetable oil production, but few genes related to oil yield have been characterized. In this study, a GDSL esterase/lipase gene, which was significantly associated with oil content, was isola...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/88400 http://hdl.handle.net/10220/45760 |
| _version_ | 1826112017695506432 |
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| author | Zhang, Yingjun Bai, Bin Lee, May Alfiko, Yuzer Suwanto, Antonius Yue, Gen Hua |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Zhang, Yingjun Bai, Bin Lee, May Alfiko, Yuzer Suwanto, Antonius Yue, Gen Hua |
| author_sort | Zhang, Yingjun |
| collection | NTU |
| description | Oil palm (Elaeis guineensis, Jacq.) is a key tropical oil crop, which provides over one third of the global vegetable oil production, but few genes related to oil yield have been characterized. In this study, a GDSL esterase/lipase gene, which was significantly associated with oil content, was isolated from oil palm and designated as EgGDSL. Its functional characterization was carried out through ectopic expression in Arabidopsis ecotype Col-0. It was shown that expression of EgGDSL in Arabidopsis led to the increased total fatty acid content by 9.5% compared with the wild type. Further analysis of the fatty acid composition revealed that stearic acid (18:0) increased in the seeds of the transgenic lines, but the levels of linoleic acid (18:2) plus 11-eicosenoic acid drastically declined. Quantitative real-time PCR (qPCR) revealed that in oil palm, EgGDSL was highly expressed in mesocarp followed by leaf, and the expression level was very low in the root. The expression level of EgGDSL gene began to increase at two months after flowering (MAF) and reached its peak by four MAF, then declined rapidly, and reached its lowest level during the mature period (6 MAF). The EgGDSL gene was more highly expressed in oil palm trees with high oil content than that with low oil content, demonstrating that the transcription level of EgGDSL correlated with the amount of oil accumulation. The gene may be valuable for engineering fatty acid metabolism in crop improvement programmes and for marker-assisted breeding. |
| first_indexed | 2024-10-01T03:00:22Z |
| format | Journal Article |
| id | ntu-10356/88400 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:00:22Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/884002023-02-28T17:02:29Z Cloning and characterization of EgGDSL, a gene associated with oil content in oil palm Zhang, Yingjun Bai, Bin Lee, May Alfiko, Yuzer Suwanto, Antonius Yue, Gen Hua School of Biological Sciences EgGDSL Gene DRNTU::Science::Biological sciences Oil Palm Oil palm (Elaeis guineensis, Jacq.) is a key tropical oil crop, which provides over one third of the global vegetable oil production, but few genes related to oil yield have been characterized. In this study, a GDSL esterase/lipase gene, which was significantly associated with oil content, was isolated from oil palm and designated as EgGDSL. Its functional characterization was carried out through ectopic expression in Arabidopsis ecotype Col-0. It was shown that expression of EgGDSL in Arabidopsis led to the increased total fatty acid content by 9.5% compared with the wild type. Further analysis of the fatty acid composition revealed that stearic acid (18:0) increased in the seeds of the transgenic lines, but the levels of linoleic acid (18:2) plus 11-eicosenoic acid drastically declined. Quantitative real-time PCR (qPCR) revealed that in oil palm, EgGDSL was highly expressed in mesocarp followed by leaf, and the expression level was very low in the root. The expression level of EgGDSL gene began to increase at two months after flowering (MAF) and reached its peak by four MAF, then declined rapidly, and reached its lowest level during the mature period (6 MAF). The EgGDSL gene was more highly expressed in oil palm trees with high oil content than that with low oil content, demonstrating that the transcription level of EgGDSL correlated with the amount of oil accumulation. The gene may be valuable for engineering fatty acid metabolism in crop improvement programmes and for marker-assisted breeding. Published version 2018-08-30T05:07:04Z 2019-12-06T17:02:27Z 2018-08-30T05:07:04Z 2019-12-06T17:02:27Z 2018 Journal Article Zhang, Y., Bai, B., Lee, M., Alfiko, Y., Suwanto, A., & Yue, G. H. (2018). Cloning and characterization of EgGDSL, a gene associated with oil content in oil palm. Scientific Reports, 8,11406-. doi:10.1038/s41598-018-29492-6 https://hdl.handle.net/10356/88400 http://hdl.handle.net/10220/45760 10.1038/s41598-018-29492-6 en Scientific Reports © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. 11 p. application/pdf |
| spellingShingle | EgGDSL Gene DRNTU::Science::Biological sciences Oil Palm Zhang, Yingjun Bai, Bin Lee, May Alfiko, Yuzer Suwanto, Antonius Yue, Gen Hua Cloning and characterization of EgGDSL, a gene associated with oil content in oil palm |
| title | Cloning and characterization of EgGDSL, a gene associated with oil content in oil palm |
| title_full | Cloning and characterization of EgGDSL, a gene associated with oil content in oil palm |
| title_fullStr | Cloning and characterization of EgGDSL, a gene associated with oil content in oil palm |
| title_full_unstemmed | Cloning and characterization of EgGDSL, a gene associated with oil content in oil palm |
| title_short | Cloning and characterization of EgGDSL, a gene associated with oil content in oil palm |
| title_sort | cloning and characterization of eggdsl a gene associated with oil content in oil palm |
| topic | EgGDSL Gene DRNTU::Science::Biological sciences Oil Palm |
| url | https://hdl.handle.net/10356/88400 http://hdl.handle.net/10220/45760 |
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