CAV-2 vector development and gene transfer in the central and peripheral nervous systems
The options available for genetic modification of cells of the central nervous system (CNS) have greatly increased in the last decade. The current panoply of viral and nonviral vectors provides multifunctional platforms to deliver expression cassettes to many structures and nuclei. These cassettes c...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/88500 http://hdl.handle.net/10220/49308 |
| _version_ | 1811680393529655296 |
|---|---|
| author | Rio, Danila del Beucher, Bertrand Lavigne, Marina Wehbi, Amani Dopeso-Reyes, Iria Gonzalez Saggio, Isabella Kremer, Eric J. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Rio, Danila del Beucher, Bertrand Lavigne, Marina Wehbi, Amani Dopeso-Reyes, Iria Gonzalez Saggio, Isabella Kremer, Eric J. |
| author_sort | Rio, Danila del |
| collection | NTU |
| description | The options available for genetic modification of cells of the central nervous system (CNS) have greatly increased in the last decade. The current panoply of viral and nonviral vectors provides multifunctional platforms to deliver expression cassettes to many structures and nuclei. These cassettes can replace defective genes, modify a given pathway perturbed by diseases, or express proteins that can be selectively activated by drugs or light to extinguish or excite neurons. This review focuses on the use of canine adenovirus type 2 (CAV-2) vectors for gene transfer to neurons in the brain, spinal cord, and peripheral nervous system. We discuss (1) recent advances in vector production, (2) why CAV-2 vectors preferentially transduce neurons, (3) the mechanism underlying their widespread distribution via retrograde axonal transport, (4) how CAV-2 vectors have been used to address structure/function, and (5) their therapeutic applications. |
| first_indexed | 2024-10-01T03:24:20Z |
| format | Journal Article |
| id | ntu-10356/88500 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:24:20Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/885002023-02-28T17:02:32Z CAV-2 vector development and gene transfer in the central and peripheral nervous systems Rio, Danila del Beucher, Bertrand Lavigne, Marina Wehbi, Amani Dopeso-Reyes, Iria Gonzalez Saggio, Isabella Kremer, Eric J. School of Biological Sciences NTU Institute of Structural Biology Adenovirus CAV-2 Science::Biological sciences The options available for genetic modification of cells of the central nervous system (CNS) have greatly increased in the last decade. The current panoply of viral and nonviral vectors provides multifunctional platforms to deliver expression cassettes to many structures and nuclei. These cassettes can replace defective genes, modify a given pathway perturbed by diseases, or express proteins that can be selectively activated by drugs or light to extinguish or excite neurons. This review focuses on the use of canine adenovirus type 2 (CAV-2) vectors for gene transfer to neurons in the brain, spinal cord, and peripheral nervous system. We discuss (1) recent advances in vector production, (2) why CAV-2 vectors preferentially transduce neurons, (3) the mechanism underlying their widespread distribution via retrograde axonal transport, (4) how CAV-2 vectors have been used to address structure/function, and (5) their therapeutic applications. Published version 2019-07-12T01:17:14Z 2019-12-06T17:04:37Z 2019-07-12T01:17:14Z 2019-12-06T17:04:37Z 2019 Journal Article Rio, D. d., Beucher, B., Lavigne, M., Wehbi, A., Dopeso-Reyes, I. G., Saggio, I., & Kremer, E. J. (2019). CAV-2 Vector Development and Gene Transfer in the Central and Peripheral Nervous Systems. Frontiers in Molecular Neuroscience, 12, 71-. doi:10.3389/fnmol.2019.00071 https://hdl.handle.net/10356/88500 http://hdl.handle.net/10220/49308 10.3389/fnmol.2019.00071 en Frontiers in Molecular Neuroscience © 2019 del Rio, Beucher, Lavigne, Wehbi, Gonzalez Dopeso-Reyes, Saggio and Kremer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 16 p. application/pdf |
| spellingShingle | Adenovirus CAV-2 Science::Biological sciences Rio, Danila del Beucher, Bertrand Lavigne, Marina Wehbi, Amani Dopeso-Reyes, Iria Gonzalez Saggio, Isabella Kremer, Eric J. CAV-2 vector development and gene transfer in the central and peripheral nervous systems |
| title | CAV-2 vector development and gene transfer in the central and peripheral nervous systems |
| title_full | CAV-2 vector development and gene transfer in the central and peripheral nervous systems |
| title_fullStr | CAV-2 vector development and gene transfer in the central and peripheral nervous systems |
| title_full_unstemmed | CAV-2 vector development and gene transfer in the central and peripheral nervous systems |
| title_short | CAV-2 vector development and gene transfer in the central and peripheral nervous systems |
| title_sort | cav 2 vector development and gene transfer in the central and peripheral nervous systems |
| topic | Adenovirus CAV-2 Science::Biological sciences |
| url | https://hdl.handle.net/10356/88500 http://hdl.handle.net/10220/49308 |
| work_keys_str_mv | AT riodaniladel cav2vectordevelopmentandgenetransferinthecentralandperipheralnervoussystems AT beucherbertrand cav2vectordevelopmentandgenetransferinthecentralandperipheralnervoussystems AT lavignemarina cav2vectordevelopmentandgenetransferinthecentralandperipheralnervoussystems AT wehbiamani cav2vectordevelopmentandgenetransferinthecentralandperipheralnervoussystems AT dopesoreyesiriagonzalez cav2vectordevelopmentandgenetransferinthecentralandperipheralnervoussystems AT saggioisabella cav2vectordevelopmentandgenetransferinthecentralandperipheralnervoussystems AT kremerericj cav2vectordevelopmentandgenetransferinthecentralandperipheralnervoussystems |