3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum
Entry of the facultative intracellular pathogen Brucella into host cells results in the formation of endosomal Brucella-containing vacuoles (eBCVs) that initially traffic along the endocytic pathway. eBCV acidification triggers the expression of a type IV secretion system that translocates bacterial...
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| 格式: | Journal Article |
| 语言: | English |
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2018
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| 在线阅读: | https://hdl.handle.net/10356/88621 http://hdl.handle.net/10220/44669 |
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| author | Sedzicki, Jaroslaw Tschon, Therese Low, Shyan Huey Willemart, Kevin Goldie, Kenneth N. Letesson, Jean-Jacques Stahlberg, Henning Dehio, Christoph |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Sedzicki, Jaroslaw Tschon, Therese Low, Shyan Huey Willemart, Kevin Goldie, Kenneth N. Letesson, Jean-Jacques Stahlberg, Henning Dehio, Christoph |
| author_sort | Sedzicki, Jaroslaw |
| collection | NTU |
| description | Entry of the facultative intracellular pathogen Brucella into host cells results in the formation of endosomal Brucella-containing vacuoles (eBCVs) that initially traffic along the endocytic pathway. eBCV acidification triggers the expression of a type IV secretion system that translocates bacterial effector proteins into host cells. This interferes with lysosomal fusion of eBCVs and supports their maturation to replicative Brucella-containing vacuoles (rBCVs). Bacteria replicate in rBCVs to large numbers, eventually occupying most of the cytoplasmic volume. As rBCV membranes tightly wrap each individual bacterium, they are constantly being expanded and remodeled during exponential bacterial growth. rBCVs are known to carry endoplasmic reticulum (ER) markers; however, the relationship of the vacuole to the genuine ER has remained elusive. Here, we have reconstructed the 3-dimensional ultrastructure of rBCVs and associated ER by correlative structured illumination microscopy (SIM) and focused ion beam/scanning electron microscopic tomography (FIB/SEM). Studying B. abortus-infected HeLa cells and trophoblasts derived from B. melitensis-infected mice, we demonstrate that rBCVs are complex and interconnected compartments that are continuous with neighboring ER cisternae, thus supporting a model that rBCVs are extensions of genuine ER. |
| first_indexed | 2024-10-01T06:19:09Z |
| format | Journal Article |
| id | ntu-10356/88621 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:19:09Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/886212020-11-01T05:23:14Z 3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum Sedzicki, Jaroslaw Tschon, Therese Low, Shyan Huey Willemart, Kevin Goldie, Kenneth N. Letesson, Jean-Jacques Stahlberg, Henning Dehio, Christoph Lee Kong Chian School of Medicine (LKCMedicine) Endoplasmic Reticulum Replicative Brucella-containing Vacuole Entry of the facultative intracellular pathogen Brucella into host cells results in the formation of endosomal Brucella-containing vacuoles (eBCVs) that initially traffic along the endocytic pathway. eBCV acidification triggers the expression of a type IV secretion system that translocates bacterial effector proteins into host cells. This interferes with lysosomal fusion of eBCVs and supports their maturation to replicative Brucella-containing vacuoles (rBCVs). Bacteria replicate in rBCVs to large numbers, eventually occupying most of the cytoplasmic volume. As rBCV membranes tightly wrap each individual bacterium, they are constantly being expanded and remodeled during exponential bacterial growth. rBCVs are known to carry endoplasmic reticulum (ER) markers; however, the relationship of the vacuole to the genuine ER has remained elusive. Here, we have reconstructed the 3-dimensional ultrastructure of rBCVs and associated ER by correlative structured illumination microscopy (SIM) and focused ion beam/scanning electron microscopic tomography (FIB/SEM). Studying B. abortus-infected HeLa cells and trophoblasts derived from B. melitensis-infected mice, we demonstrate that rBCVs are complex and interconnected compartments that are continuous with neighboring ER cisternae, thus supporting a model that rBCVs are extensions of genuine ER. Published version 2018-04-11T08:48:10Z 2019-12-06T17:07:28Z 2018-04-11T08:48:10Z 2019-12-06T17:07:28Z 2018 Journal Article Sedzicki, J., Tschon, T., Low, S. H., Willemart, K., Goldie, K. N., Letesson, J.-J., et al. (2018). 3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum. Journal of Cell Science, 131(4), jcs210799-. 0021-9533 https://hdl.handle.net/10356/88621 http://hdl.handle.net/10220/44669 10.1242/jcs.210799 en Journal of Cell Science © 2018 The Author(s) (published by The Company of Biologists Ltd.). This paper was published in Journal of Cell Science and is made available as an electronic reprint (preprint) with permission of The Company of Biologists Ltd. The published version is available at: [http://dx.doi.org/10.1242/jcs.210799]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 11 p. application/pdf |
| spellingShingle | Endoplasmic Reticulum Replicative Brucella-containing Vacuole Sedzicki, Jaroslaw Tschon, Therese Low, Shyan Huey Willemart, Kevin Goldie, Kenneth N. Letesson, Jean-Jacques Stahlberg, Henning Dehio, Christoph 3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum |
| title | 3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum |
| title_full | 3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum |
| title_fullStr | 3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum |
| title_full_unstemmed | 3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum |
| title_short | 3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum |
| title_sort | 3d correlative electron microscopy reveals continuity of brucella containing vacuoles with the endoplasmic reticulum |
| topic | Endoplasmic Reticulum Replicative Brucella-containing Vacuole |
| url | https://hdl.handle.net/10356/88621 http://hdl.handle.net/10220/44669 |
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