The metagenomics analysis of air, dust and surfaces of tropical buildings

This study focuses on systematic comparisons between 3 types of samples (dust, air, surface) commonly analyzed in environmental quality studies for better understanding on the source-sink relationships among microbial communities found in different samples. Dust, air and surface swab samples were co...

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Main Authors: Lau, Kenny Jia Xu, Uchida, Akira, Luhung, Irvan, Lim, Serene Boon Yuean, Schuster, Stephan Christoph
Other Authors: Interdisciplinary Graduate School (IGS)
Format: Conference Paper
Language:English
Published: 2019
Subjects:
Online Access:https://hdl.handle.net/10356/89002
http://hdl.handle.net/10220/49610
http://www.indoorair2018.org/
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author Lau, Kenny Jia Xu
Uchida, Akira
Luhung, Irvan
Lim, Serene Boon Yuean
Schuster, Stephan Christoph
author2 Interdisciplinary Graduate School (IGS)
author_facet Interdisciplinary Graduate School (IGS)
Lau, Kenny Jia Xu
Uchida, Akira
Luhung, Irvan
Lim, Serene Boon Yuean
Schuster, Stephan Christoph
author_sort Lau, Kenny Jia Xu
collection NTU
description This study focuses on systematic comparisons between 3 types of samples (dust, air, surface) commonly analyzed in environmental quality studies for better understanding on the source-sink relationships among microbial communities found in different samples. Dust, air and surface swab samples were collected from an indoor and its nearby outdoor locations. DNA was then extracted from the samples and direct (shotgun) metagenomic sequencing approach was applied for microbial community profiling of the samples. The current results indicate a moderate to high correlation between the surface and the settling dust samples.
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spelling ntu-10356/890022020-11-01T04:43:06Z The metagenomics analysis of air, dust and surfaces of tropical buildings Lau, Kenny Jia Xu Uchida, Akira Luhung, Irvan Lim, Serene Boon Yuean Schuster, Stephan Christoph Interdisciplinary Graduate School (IGS) INDOOR AIR 2018 - 15th Conference of the International Society of Indoor Air Quality and Climate Singapore Centre for Environmental Life Sciences and Engineering Surface Swabbing Low Biomass Metagenomics Science::Chemistry This study focuses on systematic comparisons between 3 types of samples (dust, air, surface) commonly analyzed in environmental quality studies for better understanding on the source-sink relationships among microbial communities found in different samples. Dust, air and surface swab samples were collected from an indoor and its nearby outdoor locations. DNA was then extracted from the samples and direct (shotgun) metagenomic sequencing approach was applied for microbial community profiling of the samples. The current results indicate a moderate to high correlation between the surface and the settling dust samples. MOE (Min. of Education, S’pore) Accepted version 2019-08-13T07:35:33Z 2019-12-06T17:15:39Z 2019-08-13T07:35:33Z 2019-12-06T17:15:39Z 2018 Conference Paper Lau, K. J. X., Uchida, A., Luhung, I., Lim, S. B. Y., & Schuster, S. C. (2018). The metagenomics analysis of air, dust and surfaces of tropical buildings. INDOOR AIR 2018 - 15th Conference of the International Society of Indoor Air Quality and Climate. https://hdl.handle.net/10356/89002 http://hdl.handle.net/10220/49610 http://www.indoorair2018.org/ en © 2018 The Author(s). All rights reserved. This paper was published by INDOOR AIR 2018 in INDOOR AIR 2018 - 15th Conference of the International Society of Indoor Air Quality and Climate and is made available with permission of The Author(s). 2 p. application/pdf
spellingShingle Surface Swabbing
Low Biomass Metagenomics
Science::Chemistry
Lau, Kenny Jia Xu
Uchida, Akira
Luhung, Irvan
Lim, Serene Boon Yuean
Schuster, Stephan Christoph
The metagenomics analysis of air, dust and surfaces of tropical buildings
title The metagenomics analysis of air, dust and surfaces of tropical buildings
title_full The metagenomics analysis of air, dust and surfaces of tropical buildings
title_fullStr The metagenomics analysis of air, dust and surfaces of tropical buildings
title_full_unstemmed The metagenomics analysis of air, dust and surfaces of tropical buildings
title_short The metagenomics analysis of air, dust and surfaces of tropical buildings
title_sort metagenomics analysis of air dust and surfaces of tropical buildings
topic Surface Swabbing
Low Biomass Metagenomics
Science::Chemistry
url https://hdl.handle.net/10356/89002
http://hdl.handle.net/10220/49610
http://www.indoorair2018.org/
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