Bevacizumab promotes T-cell–mediated collagen deposition in the mouse model of conjunctival scarring
Purpose: We determine the effects of bevacizumab on collagen production in a mouse model of conjunctival scarring. Methods: Experimental surgery was performed as described for the mouse model of conjunctival scarring, and bevacizumab was introduced by conjunctival injection. The capacity of bevacizu...
| Main Authors: | , , , , , , |
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| 格式: | Journal Article |
| 语言: | English |
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2019
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| 在线阅读: | https://hdl.handle.net/10356/89078 http://hdl.handle.net/10220/47675 |
| _version_ | 1826115512820563968 |
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| author | Seet, Li-Fong Toh, Li Zhen Chu, Stephanie Finger, Sharon N. Ginhoux, Florent Hong, Wanjin Wong, Tina Tzee Ling |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Seet, Li-Fong Toh, Li Zhen Chu, Stephanie Finger, Sharon N. Ginhoux, Florent Hong, Wanjin Wong, Tina Tzee Ling |
| author_sort | Seet, Li-Fong |
| collection | NTU |
| description | Purpose: We determine the effects of bevacizumab on collagen production in a mouse model of conjunctival scarring. Methods: Experimental surgery was performed as described for the mouse model of conjunctival scarring, and bevacizumab was introduced by conjunctival injection. The capacity of bevacizumab to recognize conjunctival VEGF-A was determined by ELISA. Col1a1 was measured by real-time PCR and immunoblotting. T cells and collagen were visualized by immunofluorescence and picrosirius red staining of bleb cryosections. Conjunctival CD4+ or CD8a+ T cells were counted by flow cytometry. Mouse splenic T cells were cultured with bevacizumab/IgG and their numbers, cell cycle, and collagen production were measured using a cell counter, flow cytometry, and sircol soluble collagen assay, respectively. Reconstitution experiments in severe combined immunodeficiency (SCID) mice were performed by injection of freshly isolated T cells on day 2 postoperatively. Results: Bevacizumab recognized approximately 20% of endogenous murine VEGF-A. Injection of bevacizumab raised Col1a1 expression in the blebs at mRNA and protein levels. Bevacizumab did not induce collagen in conjunctival fibroblasts, but increased CD4+ and CD8a+ cell numbers as well as collagen production by these cells. Collagen appeared to accumulate in the vicinity of T cells in the bevacizumab-treated blebs. While SCID blebs did not show elevated collagen levels, reconstitution with CD4+ or CD8a+ cells resulted in increased Col1a1 expression at mRNA and protein levels. Conclusions: Bevacizumab increased collagen production in the mouse model of conjunctival scarring. This collagen induction was mediated by T cells that were also stimulated by bevacizumab to increase in numbers. |
| first_indexed | 2024-10-01T03:56:42Z |
| format | Journal Article |
| id | ntu-10356/89078 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:56:42Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/890782023-07-14T15:52:03Z Bevacizumab promotes T-cell–mediated collagen deposition in the mouse model of conjunctival scarring Seet, Li-Fong Toh, Li Zhen Chu, Stephanie Finger, Sharon N. Ginhoux, Florent Hong, Wanjin Wong, Tina Tzee Ling School of Materials Science & Engineering Collagen DRNTU::Engineering::Materials Bevacizumab Purpose: We determine the effects of bevacizumab on collagen production in a mouse model of conjunctival scarring. Methods: Experimental surgery was performed as described for the mouse model of conjunctival scarring, and bevacizumab was introduced by conjunctival injection. The capacity of bevacizumab to recognize conjunctival VEGF-A was determined by ELISA. Col1a1 was measured by real-time PCR and immunoblotting. T cells and collagen were visualized by immunofluorescence and picrosirius red staining of bleb cryosections. Conjunctival CD4+ or CD8a+ T cells were counted by flow cytometry. Mouse splenic T cells were cultured with bevacizumab/IgG and their numbers, cell cycle, and collagen production were measured using a cell counter, flow cytometry, and sircol soluble collagen assay, respectively. Reconstitution experiments in severe combined immunodeficiency (SCID) mice were performed by injection of freshly isolated T cells on day 2 postoperatively. Results: Bevacizumab recognized approximately 20% of endogenous murine VEGF-A. Injection of bevacizumab raised Col1a1 expression in the blebs at mRNA and protein levels. Bevacizumab did not induce collagen in conjunctival fibroblasts, but increased CD4+ and CD8a+ cell numbers as well as collagen production by these cells. Collagen appeared to accumulate in the vicinity of T cells in the bevacizumab-treated blebs. While SCID blebs did not show elevated collagen levels, reconstitution with CD4+ or CD8a+ cells resulted in increased Col1a1 expression at mRNA and protein levels. Conclusions: Bevacizumab increased collagen production in the mouse model of conjunctival scarring. This collagen induction was mediated by T cells that were also stimulated by bevacizumab to increase in numbers. NRF (Natl Research Foundation, S’pore) NMRC (Natl Medical Research Council, S’pore) Published version 2019-02-15T03:56:04Z 2019-12-06T17:17:21Z 2019-02-15T03:56:04Z 2019-12-06T17:17:21Z 2018 Journal Article Seet, L.-F., Toh, L. Z., Chu, S., Finger, S. N., Ginhoux, F., Hong, W., & Wong, T. T. L. (2018). Bevacizumab Promotes T-Cell–Mediated Collagen Deposition in the Mouse Model of Conjunctival Scarring. Investigative Opthalmology & Visual Science, 59(3), 1682-. doi:10.1167/iovs.17-22694 0146-0404 https://hdl.handle.net/10356/89078 http://hdl.handle.net/10220/47675 10.1167/iovs.17-22694 en Investigative Opthalmology & Visual Science © 2018 The Authors. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. 11 p. application/pdf |
| spellingShingle | Collagen DRNTU::Engineering::Materials Bevacizumab Seet, Li-Fong Toh, Li Zhen Chu, Stephanie Finger, Sharon N. Ginhoux, Florent Hong, Wanjin Wong, Tina Tzee Ling Bevacizumab promotes T-cell–mediated collagen deposition in the mouse model of conjunctival scarring |
| title | Bevacizumab promotes T-cell–mediated collagen deposition in the mouse model of conjunctival scarring |
| title_full | Bevacizumab promotes T-cell–mediated collagen deposition in the mouse model of conjunctival scarring |
| title_fullStr | Bevacizumab promotes T-cell–mediated collagen deposition in the mouse model of conjunctival scarring |
| title_full_unstemmed | Bevacizumab promotes T-cell–mediated collagen deposition in the mouse model of conjunctival scarring |
| title_short | Bevacizumab promotes T-cell–mediated collagen deposition in the mouse model of conjunctival scarring |
| title_sort | bevacizumab promotes t cell mediated collagen deposition in the mouse model of conjunctival scarring |
| topic | Collagen DRNTU::Engineering::Materials Bevacizumab |
| url | https://hdl.handle.net/10356/89078 http://hdl.handle.net/10220/47675 |
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