A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator
Background: Effects of shear stress on endothelium are important for the normal physiology of blood vessels and are implicated in the pathogenesis of atherosclerosis. They have been extensively studied in vitro. In one paradigm, endothelial cells are cultured in devices that produce spatially varyin...
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/89080 http://hdl.handle.net/10220/46108 |
| _version_ | 1824455521478901760 |
|---|---|
| author | Ghim, Mean Pang, Kuin T. Arshad, Mehwish Wang, Xiaomeng Weinberg, Peter D. |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Ghim, Mean Pang, Kuin T. Arshad, Mehwish Wang, Xiaomeng Weinberg, Peter D. |
| author_sort | Ghim, Mean |
| collection | NTU |
| description | Background: Effects of shear stress on endothelium are important for the normal physiology of blood vessels and are implicated in the pathogenesis of atherosclerosis. They have been extensively studied in vitro. In one paradigm, endothelial cells are cultured in devices that produce spatially varying shear stress profiles, and the local profile is compared with the properties of cells at the same position. A flaw in this class of experiments is that cells exposed to a certain shear profile in one location may release mediators into the medium that alter the behaviour of cells at another location, experiencing different shear, thus obscuring or corrupting the true relation between shear and cell properties. Methods: Surface coating methods were developed for attaching cells only to some areas of culture-ware and preventing them from spreading into other regions even during prolonged culture. Results: Segmenting the growth of cells had no effect on cell shape, alignment and number per unit area compared to culturing cells in the whole well, but there were differences in tumour-necrosis-factor-α (TNF-α)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), and monocyte adherence to the monolayer. Conclusions: The results are consistent with the release of a mediator from cells exposed to high-magnitude uniaxial shear stress that has anti-inflammatory effects on activated endothelium; the mediator may be of importance in atherogenesis. Hence the new methods revealed an important property that would not have been observed without growth segmentation, suggesting that they could find more widespread application. |
| first_indexed | 2025-02-19T03:39:32Z |
| format | Journal Article |
| id | ntu-10356/89080 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:39:32Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/890802020-11-01T05:31:25Z A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator Ghim, Mean Pang, Kuin T. Arshad, Mehwish Wang, Xiaomeng Weinberg, Peter D. Lee Kong Chian School of Medicine (LKCMedicine) Segmenting Growth Endothelial Cells DRNTU::Science::Medicine Background: Effects of shear stress on endothelium are important for the normal physiology of blood vessels and are implicated in the pathogenesis of atherosclerosis. They have been extensively studied in vitro. In one paradigm, endothelial cells are cultured in devices that produce spatially varying shear stress profiles, and the local profile is compared with the properties of cells at the same position. A flaw in this class of experiments is that cells exposed to a certain shear profile in one location may release mediators into the medium that alter the behaviour of cells at another location, experiencing different shear, thus obscuring or corrupting the true relation between shear and cell properties. Methods: Surface coating methods were developed for attaching cells only to some areas of culture-ware and preventing them from spreading into other regions even during prolonged culture. Results: Segmenting the growth of cells had no effect on cell shape, alignment and number per unit area compared to culturing cells in the whole well, but there were differences in tumour-necrosis-factor-α (TNF-α)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), and monocyte adherence to the monolayer. Conclusions: The results are consistent with the release of a mediator from cells exposed to high-magnitude uniaxial shear stress that has anti-inflammatory effects on activated endothelium; the mediator may be of importance in atherogenesis. Hence the new methods revealed an important property that would not have been observed without growth segmentation, suggesting that they could find more widespread application. ASTAR (Agency for Sci., Tech. and Research, S’pore) NMRC (Natl Medical Research Council, S’pore) Published version 2018-09-26T07:54:50Z 2019-12-06T17:17:24Z 2018-09-26T07:54:50Z 2019-12-06T17:17:24Z 2018 Journal Article Ghim, M., Pang, K. T., Arshad, M., Wang, X., & Weinberg, P. D. (2018). A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator. Journal of Biological Engineering, 12(1), 15-. doi:10.1186/s13036-018-0107-6 https://hdl.handle.net/10356/89080 http://hdl.handle.net/10220/46108 10.1186/s13036-018-0107-6 en Journal of Biological Engineering © 2018 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. 13 p. application/pdf |
| spellingShingle | Segmenting Growth Endothelial Cells DRNTU::Science::Medicine Ghim, Mean Pang, Kuin T. Arshad, Mehwish Wang, Xiaomeng Weinberg, Peter D. A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator |
| title | A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator |
| title_full | A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator |
| title_fullStr | A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator |
| title_full_unstemmed | A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator |
| title_short | A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator |
| title_sort | novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti inflammatory mediator |
| topic | Segmenting Growth Endothelial Cells DRNTU::Science::Medicine |
| url | https://hdl.handle.net/10356/89080 http://hdl.handle.net/10220/46108 |
| work_keys_str_mv | AT ghimmean anovelmethodforsegmentinggrowthofcellsinshearedendothelialculturerevealsthesecretionofanantiinflammatorymediator AT pangkuint anovelmethodforsegmentinggrowthofcellsinshearedendothelialculturerevealsthesecretionofanantiinflammatorymediator AT arshadmehwish anovelmethodforsegmentinggrowthofcellsinshearedendothelialculturerevealsthesecretionofanantiinflammatorymediator AT wangxiaomeng anovelmethodforsegmentinggrowthofcellsinshearedendothelialculturerevealsthesecretionofanantiinflammatorymediator AT weinbergpeterd anovelmethodforsegmentinggrowthofcellsinshearedendothelialculturerevealsthesecretionofanantiinflammatorymediator AT ghimmean novelmethodforsegmentinggrowthofcellsinshearedendothelialculturerevealsthesecretionofanantiinflammatorymediator AT pangkuint novelmethodforsegmentinggrowthofcellsinshearedendothelialculturerevealsthesecretionofanantiinflammatorymediator AT arshadmehwish novelmethodforsegmentinggrowthofcellsinshearedendothelialculturerevealsthesecretionofanantiinflammatorymediator AT wangxiaomeng novelmethodforsegmentinggrowthofcellsinshearedendothelialculturerevealsthesecretionofanantiinflammatorymediator AT weinbergpeterd novelmethodforsegmentinggrowthofcellsinshearedendothelialculturerevealsthesecretionofanantiinflammatorymediator |