The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger
The slow and promiscuous properties of the CO2-fixing enzyme Rubisco constrain photosynthetic efficiency and have prompted the evolution of powerful CO2 concentrating mechanisms (CCMs). In eukaryotic microalgae a key strategy involves sequestration of the enzyme in the pyrenoid, a liquid non-membran...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Online Access: | https://hdl.handle.net/10356/89125 http://hdl.handle.net/10220/47672 |
| _version_ | 1826116668792766464 |
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| author | Wunder, Tobias Cheng, Steven Le Hung Lai, Soak-Kuan Li, Hoi-Yeung Mueller-Cajar, Oliver |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Wunder, Tobias Cheng, Steven Le Hung Lai, Soak-Kuan Li, Hoi-Yeung Mueller-Cajar, Oliver |
| author_sort | Wunder, Tobias |
| collection | NTU |
| description | The slow and promiscuous properties of the CO2-fixing enzyme Rubisco constrain photosynthetic efficiency and have prompted the evolution of powerful CO2 concentrating mechanisms (CCMs). In eukaryotic microalgae a key strategy involves sequestration of the enzyme in the pyrenoid, a liquid non-membranous compartment of the chloroplast stroma. Here we show using pure components that two proteins, Rubisco and the linker protein Essential Pyrenoid Component 1 (EPYC1), are both necessary and sufficient to phase separate and form liquid droplets. The phase-separated Rubisco is functional. Droplet composition is dynamic and components rapidly exchange with the bulk solution. Heterologous and chimeric Rubiscos exhibit variability in their tendency to demix with EPYC1. The ability to dissect aspects of pyrenoid biochemistry in vitro will permit us to inform and guide synthetic biology ambitions aiming to engineer microalgal CCMs into crop plants. |
| first_indexed | 2024-10-01T04:15:11Z |
| format | Journal Article |
| id | ntu-10356/89125 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:15:11Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/891252023-02-28T17:03:24Z The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger Wunder, Tobias Cheng, Steven Le Hung Lai, Soak-Kuan Li, Hoi-Yeung Mueller-Cajar, Oliver School of Biological Sciences DRNTU::Science::Biological sciences Rubisco Essential Pyrenoid Component 1 The slow and promiscuous properties of the CO2-fixing enzyme Rubisco constrain photosynthetic efficiency and have prompted the evolution of powerful CO2 concentrating mechanisms (CCMs). In eukaryotic microalgae a key strategy involves sequestration of the enzyme in the pyrenoid, a liquid non-membranous compartment of the chloroplast stroma. Here we show using pure components that two proteins, Rubisco and the linker protein Essential Pyrenoid Component 1 (EPYC1), are both necessary and sufficient to phase separate and form liquid droplets. The phase-separated Rubisco is functional. Droplet composition is dynamic and components rapidly exchange with the bulk solution. Heterologous and chimeric Rubiscos exhibit variability in their tendency to demix with EPYC1. The ability to dissect aspects of pyrenoid biochemistry in vitro will permit us to inform and guide synthetic biology ambitions aiming to engineer microalgal CCMs into crop plants. MOE (Min. of Education, S’pore) Published version 2019-02-15T02:02:14Z 2019-12-06T17:18:26Z 2019-02-15T02:02:14Z 2019-12-06T17:18:26Z 2018 Journal Article Wunder, T., Cheng, S. L. H., Lai, S.-K., Li, H.-Y., & Mueller-Cajar, O. (2018). The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger. Nature Communications, 9(1), 5076-. doi:10.1038/s41467-018-07624-w https://hdl.handle.net/10356/89125 http://hdl.handle.net/10220/47672 10.1038/s41467-018-07624-w en Nature Communications https://doi.org/10.21979/N9/RM7TRL © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 10 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Rubisco Essential Pyrenoid Component 1 Wunder, Tobias Cheng, Steven Le Hung Lai, Soak-Kuan Li, Hoi-Yeung Mueller-Cajar, Oliver The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger |
| title | The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger |
| title_full | The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger |
| title_fullStr | The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger |
| title_full_unstemmed | The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger |
| title_short | The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger |
| title_sort | phase separation underlying the pyrenoid based microalgal rubisco supercharger |
| topic | DRNTU::Science::Biological sciences Rubisco Essential Pyrenoid Component 1 |
| url | https://hdl.handle.net/10356/89125 http://hdl.handle.net/10220/47672 |
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