Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions
Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2018
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| Online Access: | https://hdl.handle.net/10356/89128 http://hdl.handle.net/10220/46116 |
| _version_ | 1811697448289042432 |
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| author | Moloudi, Reza Oh, Steve Yang, Chun Teo, Kim Leng Lam, Alan Tin-Lun Warkiani, Majid Ebrahimi Naing, May Win |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Moloudi, Reza Oh, Steve Yang, Chun Teo, Kim Leng Lam, Alan Tin-Lun Warkiani, Majid Ebrahimi Naing, May Win |
| author_sort | Moloudi, Reza |
| collection | NTU |
| description | Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio of culture environment. However, downstream purification of the harvested cell product needs to be addressed carefully due to distinctive features and fragility of these cell products. This work demonstrates a novel alternative approach which utilizes inertial focusing to separate microcarriers (MCs) from the final cell suspension. First, we systematically investigated MC focusing dynamics inside scaled-up curved channels with trapezoidal and rectangular cross-sections. A trapezoidal spiral channel with ultra-low-slope (Tan(α)=0.0375) was found to contribute to strong MC focusing (~300 < Re < ~400) while managing high MC volume fractions up to ~1.68%. Accordingly, the high-throughput trapezoidal spiral channel successfully separated MCs from hMSC suspension with total cell yield~94% (after two passes) at a high volumetric flow rate of ~30 mL/min (Re~326.5). |
| first_indexed | 2024-10-01T07:55:25Z |
| format | Journal Article |
| id | ntu-10356/89128 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:55:25Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/891282023-03-04T17:16:44Z Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions Moloudi, Reza Oh, Steve Yang, Chun Teo, Kim Leng Lam, Alan Tin-Lun Warkiani, Majid Ebrahimi Naing, May Win School of Mechanical and Aerospace Engineering Microcarriers Mesenchymal Stem Cell DRNTU::Engineering::Bioengineering Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio of culture environment. However, downstream purification of the harvested cell product needs to be addressed carefully due to distinctive features and fragility of these cell products. This work demonstrates a novel alternative approach which utilizes inertial focusing to separate microcarriers (MCs) from the final cell suspension. First, we systematically investigated MC focusing dynamics inside scaled-up curved channels with trapezoidal and rectangular cross-sections. A trapezoidal spiral channel with ultra-low-slope (Tan(α)=0.0375) was found to contribute to strong MC focusing (~300 < Re < ~400) while managing high MC volume fractions up to ~1.68%. Accordingly, the high-throughput trapezoidal spiral channel successfully separated MCs from hMSC suspension with total cell yield~94% (after two passes) at a high volumetric flow rate of ~30 mL/min (Re~326.5). ASTAR (Agency for Sci., Tech. and Research, S’pore) Published version 2018-09-27T03:07:35Z 2019-12-06T17:18:30Z 2018-09-27T03:07:35Z 2019-12-06T17:18:30Z 2018 Journal Article Moloudi, R., Oh, S., Yang, C., Teo, K. L., Lam, A. T.-L., Warkiani, M. E., & Naing, M. W. (2018). Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions. Scientific Reports, 8, 12481-. 2045-2322 https://hdl.handle.net/10356/89128 http://hdl.handle.net/10220/46116 10.1038/s41598-018-31019-y en Scientific Reports © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 10 p. application/pdf |
| spellingShingle | Microcarriers Mesenchymal Stem Cell DRNTU::Engineering::Bioengineering Moloudi, Reza Oh, Steve Yang, Chun Teo, Kim Leng Lam, Alan Tin-Lun Warkiani, Majid Ebrahimi Naing, May Win Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions |
| title | Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions |
| title_full | Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions |
| title_fullStr | Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions |
| title_full_unstemmed | Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions |
| title_short | Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions |
| title_sort | inertial based filtration method for removal of microcarriers from mesenchymal stem cell suspensions |
| topic | Microcarriers Mesenchymal Stem Cell DRNTU::Engineering::Bioengineering |
| url | https://hdl.handle.net/10356/89128 http://hdl.handle.net/10220/46116 |
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