Effects of pupil filter patterns in line-scan focal modulation microscopy
Line-scan focal modulation microscopy (LSFMM) is an emerging imaging technique that affords high imaging speed and good optical sectioning at the same time. We present a systematic investigation into optimal design of the pupil filter for LSFMM in an attempt to achieve the best performance in terms...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/89144 http://hdl.handle.net/10220/44817 |
| _version_ | 1826125191867006976 |
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| author | Shen, Shuhao Pant, Shilpa Chen, Rui Chen, Nanguang |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Shen, Shuhao Pant, Shilpa Chen, Rui Chen, Nanguang |
| author_sort | Shen, Shuhao |
| collection | NTU |
| description | Line-scan focal modulation microscopy (LSFMM) is an emerging imaging technique that affords high imaging speed and good optical sectioning at the same time. We present a systematic investigation into optimal design of the pupil filter for LSFMM in an attempt to achieve the best performance in terms of spatial resolutions, optical sectioning, and modulation depth. Scalar diffraction theory was used to compute light propagation and distribution in the system and theoretical predictions on system performance, which were then compared with experimental results. |
| first_indexed | 2024-10-01T06:32:41Z |
| format | Journal Article |
| id | ntu-10356/89144 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:32:41Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/891442020-03-07T14:02:36Z Effects of pupil filter patterns in line-scan focal modulation microscopy Shen, Shuhao Pant, Shilpa Chen, Rui Chen, Nanguang School of Electrical and Electronic Engineering Fluorescence Microscopy Line-scan Line-scan focal modulation microscopy (LSFMM) is an emerging imaging technique that affords high imaging speed and good optical sectioning at the same time. We present a systematic investigation into optimal design of the pupil filter for LSFMM in an attempt to achieve the best performance in terms of spatial resolutions, optical sectioning, and modulation depth. Scalar diffraction theory was used to compute light propagation and distribution in the system and theoretical predictions on system performance, which were then compared with experimental results. NMRC (Natl Medical Research Council, S’pore) Published version 2018-05-17T04:32:18Z 2019-12-06T17:18:50Z 2018-05-17T04:32:18Z 2019-12-06T17:18:50Z 2018 Journal Article Shen, S., Pant, S., Chen, R., & Chen, N. (2018). Effects of pupil filter patterns in line-scan focal modulation microscopy. Journal of Biomedical Optics, 23(3), 036008-. 1083-3668 https://hdl.handle.net/10356/89144 http://hdl.handle.net/10220/44817 10.1117/1.JBO.23.3.036008 en Journal of Biomedical Optics © 2018 Society of Photo-optical Instrumentation Engineers. This paper was published in Journal of Biomedical Optics and is made available as an electronic reprint (preprint) with permission of Society of Photo-optical Instrumentation Engineers. The published version is available at [http://dx.doi.org/10.1117/1.JBO.23.3.036008]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 6 p. application/pdf |
| spellingShingle | Fluorescence Microscopy Line-scan Shen, Shuhao Pant, Shilpa Chen, Rui Chen, Nanguang Effects of pupil filter patterns in line-scan focal modulation microscopy |
| title | Effects of pupil filter patterns in line-scan focal modulation microscopy |
| title_full | Effects of pupil filter patterns in line-scan focal modulation microscopy |
| title_fullStr | Effects of pupil filter patterns in line-scan focal modulation microscopy |
| title_full_unstemmed | Effects of pupil filter patterns in line-scan focal modulation microscopy |
| title_short | Effects of pupil filter patterns in line-scan focal modulation microscopy |
| title_sort | effects of pupil filter patterns in line scan focal modulation microscopy |
| topic | Fluorescence Microscopy Line-scan |
| url | https://hdl.handle.net/10356/89144 http://hdl.handle.net/10220/44817 |
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