Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids
A model immunoassay test system is proposed based on metal enhanced photoluminescence to analyze low concentrations of alpha-fetoprotein, a tumor marker. Antigen-antibody reaction was performed on polystyrene plates coated with silver nanoparticles to increase sensitivity of fluorescent immunoassay...
| Autori principali: | , , , , , , , |
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| Natura: | Journal Article |
| Lingua: | English |
| Pubblicazione: |
2018
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| Accesso online: | https://hdl.handle.net/10356/89233 http://hdl.handle.net/10220/46198 |
| _version_ | 1826110790146457600 |
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| author | Vaschenko, Svetlana Ramanenka, Andrey Kulakovich, Olga Muravitskaya, Alina Guzatov, Dmitry Lunevich, Anatoly Glukhov, Yuri Gaponenko, Sergey |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Vaschenko, Svetlana Ramanenka, Andrey Kulakovich, Olga Muravitskaya, Alina Guzatov, Dmitry Lunevich, Anatoly Glukhov, Yuri Gaponenko, Sergey |
| author_sort | Vaschenko, Svetlana |
| collection | NTU |
| description | A model immunoassay test system is proposed based on metal enhanced photoluminescence to analyze low concentrations of alpha-fetoprotein, a tumor marker. Antigen-antibody reaction was performed on polystyrene plates coated with silver nanoparticles to increase sensitivity of fluorescent immunoassay and signal-to-noise ratio as compared to silver-free system. The proposed test system model uses layer-by-layer assembly approach, LED excitation and nanowatt photodetection set-up, so it is characterized by smaller probe volume, fast analysis and simplicity. The proposed model system offers alpha-fetoprotein detection at concentrations used in clinical practice. Photostability and photoluminescence enhancement for labeled alpha-fetoprotein antibodies on a silver substrate were found to depend on antibodies concentration. Labeled antibodies photostability on substrates was found to deviate within 20% as compared to silver-free samples whereas photoluminescence intensity enhancement is typically in the range of 6-8 times. |
| first_indexed | 2024-10-01T02:39:55Z |
| format | Journal Article |
| id | ntu-10356/89233 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:39:55Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/892332020-03-07T14:02:37Z Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids Vaschenko, Svetlana Ramanenka, Andrey Kulakovich, Olga Muravitskaya, Alina Guzatov, Dmitry Lunevich, Anatoly Glukhov, Yuri Gaponenko, Sergey School of Electrical and Electronic Engineering Silver Nanoparticles DRNTU::Engineering::Electrical and electronic engineering Pasmon-enhanced Fluorescence A model immunoassay test system is proposed based on metal enhanced photoluminescence to analyze low concentrations of alpha-fetoprotein, a tumor marker. Antigen-antibody reaction was performed on polystyrene plates coated with silver nanoparticles to increase sensitivity of fluorescent immunoassay and signal-to-noise ratio as compared to silver-free system. The proposed test system model uses layer-by-layer assembly approach, LED excitation and nanowatt photodetection set-up, so it is characterized by smaller probe volume, fast analysis and simplicity. The proposed model system offers alpha-fetoprotein detection at concentrations used in clinical practice. Photostability and photoluminescence enhancement for labeled alpha-fetoprotein antibodies on a silver substrate were found to depend on antibodies concentration. Labeled antibodies photostability on substrates was found to deviate within 20% as compared to silver-free samples whereas photoluminescence intensity enhancement is typically in the range of 6-8 times. Published version 2018-10-02T09:32:44Z 2019-12-06T17:20:49Z 2018-10-02T09:32:44Z 2019-12-06T17:20:49Z 2016 Journal Article Vaschenko, S., Ramanenka, A., Kulakovich, O., Muravitskaya, A., Guzatov, D., Lunevich, A., ... Gaponenko, S. (2016). Enhancement of Labeled Alpha-fetoprotein Antibodies and Antigen-antibody Complexes Fluorescence with Silver Nanocolloids. Procedia Engineering, 140, 57-66. doi : 10.1016/j.proeng.2015.08.1111 1877-7058 https://hdl.handle.net/10356/89233 http://hdl.handle.net/10220/46198 10.1016/j.proeng.2015.08.1111 en Procedia Engineering © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/). Selection and/or peer-review under responsibility of the scientific committee of Symposium 2015 ICMAT 10 p. application/pdf |
| spellingShingle | Silver Nanoparticles DRNTU::Engineering::Electrical and electronic engineering Pasmon-enhanced Fluorescence Vaschenko, Svetlana Ramanenka, Andrey Kulakovich, Olga Muravitskaya, Alina Guzatov, Dmitry Lunevich, Anatoly Glukhov, Yuri Gaponenko, Sergey Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids |
| title | Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids |
| title_full | Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids |
| title_fullStr | Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids |
| title_full_unstemmed | Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids |
| title_short | Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids |
| title_sort | enhancement of labeled alpha fetoprotein antibodies and antigen antibody complexes fluorescence with silver nanocolloids |
| topic | Silver Nanoparticles DRNTU::Engineering::Electrical and electronic engineering Pasmon-enhanced Fluorescence |
| url | https://hdl.handle.net/10356/89233 http://hdl.handle.net/10220/46198 |
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