Photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes
Due to the embedded nature of the lamina cribrosa (LC) microcapillary network, conventional imaging techniques have failed to obtain the high-resolution images needed to assess the perfusion state of the LC. In this study, both optical resolution (OR) and acoustic resolution (AR) photoacoustic micro...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/89548 http://hdl.handle.net/10220/46775 |
| _version_ | 1826120449879179264 |
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| author | Pramanik, Manojit Moothanchery, Mohesh Schmetterer, Leopold Chuangsuwanich, Thanadet Girard, Michaël J. A. Tsz, Alvan Chung Yan |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Pramanik, Manojit Moothanchery, Mohesh Schmetterer, Leopold Chuangsuwanich, Thanadet Girard, Michaël J. A. Tsz, Alvan Chung Yan |
| author_sort | Pramanik, Manojit |
| collection | NTU |
| description | Due to the embedded nature of the lamina cribrosa (LC) microcapillary network, conventional imaging techniques have failed to obtain the high-resolution images needed to assess the perfusion state of the LC. In this study, both optical resolution (OR) and acoustic resolution (AR) photoacoustic microscopy (PAM) techniques were used to obtain static and dynamic information about LC perfusion in ex vivo porcine eyes. The OR-PAM system could resolve a perfused LC microcapillary network with a lateral resolution of 4.2 μm and also provided good depth information (33 μm axial resolution) to visualize through-thickness vascular variations. The AR-PAM system was capable of detecting time-dependent perfusion variations. This study represents the first step towards
using an emerging imaging modality (PAM) to study the LC’s perfusion, which could be a basis for further investigation of the hemodynamic aspects of glaucomatous optic neuropathy. |
| first_indexed | 2024-10-01T05:16:58Z |
| format | Journal Article |
| id | ntu-10356/89548 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:16:58Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/895482021-02-23T01:04:48Z Photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes Pramanik, Manojit Moothanchery, Mohesh Schmetterer, Leopold Chuangsuwanich, Thanadet Girard, Michaël J. A. Tsz, Alvan Chung Yan School of Chemical and Biomedical Engineering Lee Kong Chian School of Medicine (LKCMedicine) Photoacoustic Imaging Ophthalmic Optics and Devices DRNTU::Engineering::Bioengineering Due to the embedded nature of the lamina cribrosa (LC) microcapillary network, conventional imaging techniques have failed to obtain the high-resolution images needed to assess the perfusion state of the LC. In this study, both optical resolution (OR) and acoustic resolution (AR) photoacoustic microscopy (PAM) techniques were used to obtain static and dynamic information about LC perfusion in ex vivo porcine eyes. The OR-PAM system could resolve a perfused LC microcapillary network with a lateral resolution of 4.2 μm and also provided good depth information (33 μm axial resolution) to visualize through-thickness vascular variations. The AR-PAM system was capable of detecting time-dependent perfusion variations. This study represents the first step towards using an emerging imaging modality (PAM) to study the LC’s perfusion, which could be a basis for further investigation of the hemodynamic aspects of glaucomatous optic neuropathy. Accepted version 2018-12-03T08:51:55Z 2019-12-06T17:28:07Z 2018-12-03T08:51:55Z 2019-12-06T17:28:07Z 2018 2018 Journal Article Chuangsuwanich, T., Moothanchery, M., Tsz, A. C. Y., Schmetterer, L., Girard, M. J. A., & Pramanik, M. (2018). Photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes. Applied Optics, 57(17), 4865-. doi:0.1364/AO.57.004865 1559-128X https://hdl.handle.net/10356/89548 http://hdl.handle.net/10220/46775 208297 208297 10.1364/AO.57.004865 208297 208297 en Applied Optics © 2018 Optical Society of America (OSA). This is the author created version of a work that has been peer reviewed and accepted for publication by Applied Optics, Optical Society of America (OSA). It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1364/AO.57.004865]. 7 p. application/pdf |
| spellingShingle | Photoacoustic Imaging Ophthalmic Optics and Devices DRNTU::Engineering::Bioengineering Pramanik, Manojit Moothanchery, Mohesh Schmetterer, Leopold Chuangsuwanich, Thanadet Girard, Michaël J. A. Tsz, Alvan Chung Yan Photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes |
| title | Photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes |
| title_full | Photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes |
| title_fullStr | Photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes |
| title_full_unstemmed | Photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes |
| title_short | Photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes |
| title_sort | photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes |
| topic | Photoacoustic Imaging Ophthalmic Optics and Devices DRNTU::Engineering::Bioengineering |
| url | https://hdl.handle.net/10356/89548 http://hdl.handle.net/10220/46775 |
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