The application of ultrasound in 3D bio-printing
Three-dimensional (3D) bioprinting is an emerging and promising technology in tissue engineering to construct tissues and organs for implantation. Alignment of self-assembly cell spheroids that are used as bioink could be very accurate after droplet ejection from bioprinter. Complex and heterogeneou...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/89590 http://hdl.handle.net/10220/46286 |
| _version_ | 1811691815945895936 |
|---|---|
| author | Zhou, Yufeng |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Zhou, Yufeng |
| author_sort | Zhou, Yufeng |
| collection | NTU |
| description | Three-dimensional (3D) bioprinting is an emerging and promising technology in tissue engineering to construct tissues and organs for implantation. Alignment of self-assembly cell spheroids that are used as bioink could be very accurate after droplet ejection from bioprinter. Complex and heterogeneous tissue structures could be built using rapid additive manufacture technology and multiple cell lines. Effective vascularization in the engineered tissue samples is critical in any clinical application. In this review paper, the current technologies and processing steps (such as printing, preparation of bioink, cross-linking, tissue fusion and maturation) in 3D bio-printing are introduced, and their specifications are compared with each other. In addition, the application of ultrasound in this novel field is also introduced. Cells experience acoustic radiation force in ultrasound standing wave field (USWF) and then accumulate at the pressure node at low acoustic pressure. Formation of cell spheroids by this method is within minutes with uniform size and homogeneous cell distribution. Neovessel formation from USWF-induced endothelial cell spheroids is significant. Low-intensity ultrasound could enhance the proliferation and differentiation of stem cells. Its use is at low cost and compatible with current bioreactor. In summary, ultrasound application in 3D bio-printing may solve some challenges and enhance the outcomes. |
| first_indexed | 2024-10-01T06:25:54Z |
| format | Journal Article |
| id | ntu-10356/89590 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:25:54Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/895902020-09-26T22:07:08Z The application of ultrasound in 3D bio-printing Zhou, Yufeng School of Mechanical and Aerospace Engineering Singapore Centre for 3D Printing Bioink DRNTU::Engineering::Mechanical engineering Three-dimensional Bio-printing Three-dimensional (3D) bioprinting is an emerging and promising technology in tissue engineering to construct tissues and organs for implantation. Alignment of self-assembly cell spheroids that are used as bioink could be very accurate after droplet ejection from bioprinter. Complex and heterogeneous tissue structures could be built using rapid additive manufacture technology and multiple cell lines. Effective vascularization in the engineered tissue samples is critical in any clinical application. In this review paper, the current technologies and processing steps (such as printing, preparation of bioink, cross-linking, tissue fusion and maturation) in 3D bio-printing are introduced, and their specifications are compared with each other. In addition, the application of ultrasound in this novel field is also introduced. Cells experience acoustic radiation force in ultrasound standing wave field (USWF) and then accumulate at the pressure node at low acoustic pressure. Formation of cell spheroids by this method is within minutes with uniform size and homogeneous cell distribution. Neovessel formation from USWF-induced endothelial cell spheroids is significant. Low-intensity ultrasound could enhance the proliferation and differentiation of stem cells. Its use is at low cost and compatible with current bioreactor. In summary, ultrasound application in 3D bio-printing may solve some challenges and enhance the outcomes. Published version 2018-10-11T05:51:58Z 2019-12-06T17:29:05Z 2018-10-11T05:51:58Z 2019-12-06T17:29:05Z 2016 Journal Article Zhou, Y. (2016). The application of ultrasound in 3D bio-printing. Molecules, 21(5), 590-. doi:10.3390/molecules21050590 1420-3049 https://hdl.handle.net/10356/89590 http://hdl.handle.net/10220/46286 10.3390/molecules21050590 en Molecules © 2016 by the author; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). 25 p. application/pdf |
| spellingShingle | Bioink DRNTU::Engineering::Mechanical engineering Three-dimensional Bio-printing Zhou, Yufeng The application of ultrasound in 3D bio-printing |
| title | The application of ultrasound in 3D bio-printing |
| title_full | The application of ultrasound in 3D bio-printing |
| title_fullStr | The application of ultrasound in 3D bio-printing |
| title_full_unstemmed | The application of ultrasound in 3D bio-printing |
| title_short | The application of ultrasound in 3D bio-printing |
| title_sort | application of ultrasound in 3d bio printing |
| topic | Bioink DRNTU::Engineering::Mechanical engineering Three-dimensional Bio-printing |
| url | https://hdl.handle.net/10356/89590 http://hdl.handle.net/10220/46286 |
| work_keys_str_mv | AT zhouyufeng theapplicationofultrasoundin3dbioprinting AT zhouyufeng applicationofultrasoundin3dbioprinting |