1064 nm acoustic resolution photoacoustic microscopy
Photoacoustic imaging is a noninvasive imaging technique having the advantages of high-optical contrast and good acoustic resolution at improved imaging depths. Light transport in biological tissues is mainly characterized by strong optical scattering and absorption. Photoacoustic microscopy is capa...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Online Access: | https://hdl.handle.net/10356/89744 http://hdl.handle.net/10220/49051 |
| _version_ | 1811679007052136448 |
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| author | Periyasamy, Vijitha Das, Nandan Sharma, Arunima Pramanik, Manojit |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Periyasamy, Vijitha Das, Nandan Sharma, Arunima Pramanik, Manojit |
| author_sort | Periyasamy, Vijitha |
| collection | NTU |
| description | Photoacoustic imaging is a noninvasive imaging technique having the advantages of high-optical contrast and good acoustic resolution at improved imaging depths. Light transport in biological tissues is mainly characterized by strong optical scattering and absorption. Photoacoustic microscopy is capable of achieving highresolution images at greater depth compared to conventional optical microscopy methods. In this work, we have developed a high-resolution, acoustic resolution photoacoustic microscopy (AR-PAM) system in the near infra-red (NIR) window
II (NIR-II, eg, 1064 nm) for deep tissue imaging. Higher imaging depth is achieved as the tissue scattering at 1064 nm is lesser compared to visible or near infrared window-I (NIR-I). Our developed system can provide a lateral resolution of 130 μm, axial resolution of 57 μm, and image up to 11 mm deep in biological tissues. This 1064-AR-PAM system was used for imaging sentinel lymph node and
the lymph vessel in rat. Urinary bladder of rat filled with black ink was also imaged
to validate the feasibility of the developed system to study deeply seated organs. |
| first_indexed | 2024-10-01T03:02:18Z |
| format | Journal Article |
| id | ntu-10356/89744 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:02:18Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/897442023-12-29T06:46:01Z 1064 nm acoustic resolution photoacoustic microscopy Periyasamy, Vijitha Das, Nandan Sharma, Arunima Pramanik, Manojit School of Chemical and Biomedical Engineering Acoustic Resolution Photoacoustic Microscopy Photoacoustic Imaging Engineering::Chemical engineering Photoacoustic imaging is a noninvasive imaging technique having the advantages of high-optical contrast and good acoustic resolution at improved imaging depths. Light transport in biological tissues is mainly characterized by strong optical scattering and absorption. Photoacoustic microscopy is capable of achieving highresolution images at greater depth compared to conventional optical microscopy methods. In this work, we have developed a high-resolution, acoustic resolution photoacoustic microscopy (AR-PAM) system in the near infra-red (NIR) window II (NIR-II, eg, 1064 nm) for deep tissue imaging. Higher imaging depth is achieved as the tissue scattering at 1064 nm is lesser compared to visible or near infrared window-I (NIR-I). Our developed system can provide a lateral resolution of 130 μm, axial resolution of 57 μm, and image up to 11 mm deep in biological tissues. This 1064-AR-PAM system was used for imaging sentinel lymph node and the lymph vessel in rat. Urinary bladder of rat filled with black ink was also imaged to validate the feasibility of the developed system to study deeply seated organs. MOE (Min. of Education, S’pore) Accepted version 2019-07-01T07:58:46Z 2019-12-06T17:32:28Z 2019-07-01T07:58:46Z 2019-12-06T17:32:28Z 2019 2019 Journal Article Periyasamy, V., Das, N., Sharma, A., & Pramanik, M. (2019). 1064 nm acoustic resolution photoacoustic microscopy. Journal of Biophotonics, 12(5), e201800357-. doi:10.1002/jbio.201800357 1864-063X https://hdl.handle.net/10356/89744 http://hdl.handle.net/10220/49051 10.1002/jbio.201800357 212112 en Journal of Biophotonics This is the peer reviewed version of the following article: Periyasamy, V., Das, N., Sharma, A., & Pramanik, M. (2019). 1064 nm acoustic resolution photoacoustic microscopy. Journal of Biophotonics, 12(5), e201800357-. doi:10.1002/jbio.201800357, which has been published in final form at https://doi.org/10.1002/jbio.201800357. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. 24 p. application/pdf |
| spellingShingle | Acoustic Resolution Photoacoustic Microscopy Photoacoustic Imaging Engineering::Chemical engineering Periyasamy, Vijitha Das, Nandan Sharma, Arunima Pramanik, Manojit 1064 nm acoustic resolution photoacoustic microscopy |
| title | 1064 nm acoustic resolution photoacoustic microscopy |
| title_full | 1064 nm acoustic resolution photoacoustic microscopy |
| title_fullStr | 1064 nm acoustic resolution photoacoustic microscopy |
| title_full_unstemmed | 1064 nm acoustic resolution photoacoustic microscopy |
| title_short | 1064 nm acoustic resolution photoacoustic microscopy |
| title_sort | 1064 nm acoustic resolution photoacoustic microscopy |
| topic | Acoustic Resolution Photoacoustic Microscopy Photoacoustic Imaging Engineering::Chemical engineering |
| url | https://hdl.handle.net/10356/89744 http://hdl.handle.net/10220/49051 |
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