A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
3D bioprinting is an emerging technology that enables fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requirements (cell-compatibility, printability, structural stability post-printing and bio-fu...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2018
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| Online Access: | https://hdl.handle.net/10356/89957 http://hdl.handle.net/10220/47176 |
| _version_ | 1826112675711549440 |
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| author | Zhou, Miaomiao Lee, Bae Hoon Tan, Lay Poh |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Zhou, Miaomiao Lee, Bae Hoon Tan, Lay Poh |
| author_sort | Zhou, Miaomiao |
| collection | NTU |
| description | 3D bioprinting is an emerging technology that enables fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requirements (cell-compatibility, printability, structural stability post-printing and bio-functionality to regulate cell behaviours) suitable for printing. Gelatin methacryloyl (GelMA) has recently emerged as an attractive biomaterial in tissue engineering because it satisfies the requirements of bio-functionality and mechanical tunability. However, the poor rheological property such as low viscosity at body temperature inhibits its application in 3D bioprinting. In this work, an enzymatic crosslinking method triggered by Ca2+-independent microbial transglutaminase (MTGase) was introduced to catalyse isopeptide bonds formation between chains of GelMA, which could improve its rheological behaviours, specifically its viscosity. By combining enzymatic crosslinking and photo crosslinking, it is possible to tune the solution viscosity and quickly stabilize the gelatin macromolecules at the same time. The results showed that the enzymatic crosslinking can increase the solution viscosity. Subsequent photo crosslinking could aid in fast stabilization of the structure and make handling easy. |
| first_indexed | 2024-10-01T03:10:58Z |
| format | Journal Article |
| id | ntu-10356/89957 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:10:58Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/899572023-07-14T15:52:31Z A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl Zhou, Miaomiao Lee, Bae Hoon Tan, Lay Poh School of Materials Science & Engineering Singapore Centre for 3D Printing DRNTU::Engineering::Materials Microbial Transglutaminase Enzymatic Crosslinking 3D bioprinting is an emerging technology that enables fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requirements (cell-compatibility, printability, structural stability post-printing and bio-functionality to regulate cell behaviours) suitable for printing. Gelatin methacryloyl (GelMA) has recently emerged as an attractive biomaterial in tissue engineering because it satisfies the requirements of bio-functionality and mechanical tunability. However, the poor rheological property such as low viscosity at body temperature inhibits its application in 3D bioprinting. In this work, an enzymatic crosslinking method triggered by Ca2+-independent microbial transglutaminase (MTGase) was introduced to catalyse isopeptide bonds formation between chains of GelMA, which could improve its rheological behaviours, specifically its viscosity. By combining enzymatic crosslinking and photo crosslinking, it is possible to tune the solution viscosity and quickly stabilize the gelatin macromolecules at the same time. The results showed that the enzymatic crosslinking can increase the solution viscosity. Subsequent photo crosslinking could aid in fast stabilization of the structure and make handling easy. NRF (Natl Research Foundation, S’pore) Published version 2018-12-21T07:29:29Z 2019-12-06T17:37:26Z 2018-12-21T07:29:29Z 2019-12-06T17:37:26Z 2017 Journal Article Zhou, M., Lee, B. H., & Tan, L. P. (2017). A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl. International Journal of Bioprinting, 3(2), 130-137. doi:10.18063/IJB.2017.02.003 https://hdl.handle.net/10356/89957 http://hdl.handle.net/10220/47176 10.18063/IJB.2017.02.003 en International Journal of Bioprinting © 2017 Miaomiao Zhou, et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/bync/4.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 8 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Materials Microbial Transglutaminase Enzymatic Crosslinking Zhou, Miaomiao Lee, Bae Hoon Tan, Lay Poh A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl |
| title | A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl |
| title_full | A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl |
| title_fullStr | A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl |
| title_full_unstemmed | A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl |
| title_short | A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl |
| title_sort | dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl |
| topic | DRNTU::Engineering::Materials Microbial Transglutaminase Enzymatic Crosslinking |
| url | https://hdl.handle.net/10356/89957 http://hdl.handle.net/10220/47176 |
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