Integrated analysis of the Plasmodium species transcriptome
The genome sequence available for different Plasmodium species is a valuable resource for understanding malaria parasite biology. However, comparative genomics on its own cannot fully explain all the species-specific differences which suggests that other genomic aspects such as regulation of gene ex...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/89991 http://hdl.handle.net/10220/46472 |
| _version_ | 1824453580926484480 |
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| author | Hoo, Regina Zhu, Lei Amaladoss, Anburaj Mok, Sachel Natalang, Onguma Lapp, Stacey A. Hu, Guangan Liew, Kingsley Galinski, Mary R. Bozdech, Zbynek Preiser, Peter Rainer |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Hoo, Regina Zhu, Lei Amaladoss, Anburaj Mok, Sachel Natalang, Onguma Lapp, Stacey A. Hu, Guangan Liew, Kingsley Galinski, Mary R. Bozdech, Zbynek Preiser, Peter Rainer |
| author_sort | Hoo, Regina |
| collection | NTU |
| description | The genome sequence available for different Plasmodium species is a valuable resource for understanding malaria parasite biology. However, comparative genomics on its own cannot fully explain all the species-specific differences which suggests that other genomic aspects such as regulation of gene expression play an important role in defining species-specific characteristics. Here, we developed a comprehensive approach to measure transcriptional changes of the evolutionary conserved syntenic orthologs during the intraerythrocytic developmental cycle across six Plasmodium species. We show significant transcriptional constraint at the mid-developmental stage of Plasmodium species while the earliest stages of parasite development display the greatest transcriptional variation associated with critical functional processes. Modeling of the evolutionary relationship based on changes in transcriptional profile reveal a phylogeny pattern of the Plasmodium species that strictly follows its mammalian hosts. In addition, the work shows that transcriptional conserved orthologs represent potential future targets for anti-malaria intervention as they would be expected to carry out key essential functions within the parasites. This work provides an integrated analysis of orthologous transcriptome, which aims to provide insights into the Plasmodium evolution thereby establishing a framework to explore complex pathways and drug discovery in Plasmodium species with broad host range. |
| first_indexed | 2025-02-19T03:08:41Z |
| format | Journal Article |
| id | ntu-10356/89991 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:08:41Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/899912023-02-28T17:03:22Z Integrated analysis of the Plasmodium species transcriptome Hoo, Regina Zhu, Lei Amaladoss, Anburaj Mok, Sachel Natalang, Onguma Lapp, Stacey A. Hu, Guangan Liew, Kingsley Galinski, Mary R. Bozdech, Zbynek Preiser, Peter Rainer School of Biological Sciences DRNTU::Science::Biological sciences Comparative Transcriptomics Plasmodium Species The genome sequence available for different Plasmodium species is a valuable resource for understanding malaria parasite biology. However, comparative genomics on its own cannot fully explain all the species-specific differences which suggests that other genomic aspects such as regulation of gene expression play an important role in defining species-specific characteristics. Here, we developed a comprehensive approach to measure transcriptional changes of the evolutionary conserved syntenic orthologs during the intraerythrocytic developmental cycle across six Plasmodium species. We show significant transcriptional constraint at the mid-developmental stage of Plasmodium species while the earliest stages of parasite development display the greatest transcriptional variation associated with critical functional processes. Modeling of the evolutionary relationship based on changes in transcriptional profile reveal a phylogeny pattern of the Plasmodium species that strictly follows its mammalian hosts. In addition, the work shows that transcriptional conserved orthologs represent potential future targets for anti-malaria intervention as they would be expected to carry out key essential functions within the parasites. This work provides an integrated analysis of orthologous transcriptome, which aims to provide insights into the Plasmodium evolution thereby establishing a framework to explore complex pathways and drug discovery in Plasmodium species with broad host range. NMRC (Natl Medical Research Council, S’pore) Published version 2018-10-29T09:03:46Z 2019-12-06T17:38:11Z 2018-10-29T09:03:46Z 2019-12-06T17:38:11Z 2016 Journal Article Hoo, R., Zhu, L., Amaladoss, A., Mok, S., Natalang, O., Lapp, S. A., . . . Preiser, P. R. (2016). Integrated analysis of the Plasmodium species transcriptome. EBioMedicine, 7(1), 255-266. doi:10.1016/j.ebiom.2016.04.011 https://hdl.handle.net/10356/89991 http://hdl.handle.net/10220/46472 10.1016/j.ebiom.2016.04.011 27322479 en EBioMedicine https://doi.org/10.21979/N9/UBCFAP © 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). 12 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Comparative Transcriptomics Plasmodium Species Hoo, Regina Zhu, Lei Amaladoss, Anburaj Mok, Sachel Natalang, Onguma Lapp, Stacey A. Hu, Guangan Liew, Kingsley Galinski, Mary R. Bozdech, Zbynek Preiser, Peter Rainer Integrated analysis of the Plasmodium species transcriptome |
| title | Integrated analysis of the Plasmodium species transcriptome |
| title_full | Integrated analysis of the Plasmodium species transcriptome |
| title_fullStr | Integrated analysis of the Plasmodium species transcriptome |
| title_full_unstemmed | Integrated analysis of the Plasmodium species transcriptome |
| title_short | Integrated analysis of the Plasmodium species transcriptome |
| title_sort | integrated analysis of the plasmodium species transcriptome |
| topic | DRNTU::Science::Biological sciences Comparative Transcriptomics Plasmodium Species |
| url | https://hdl.handle.net/10356/89991 http://hdl.handle.net/10220/46472 |
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