Elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide BRBR-NH 2
Linear antimicrobial peptides, with their rapid bactericidal mode of action, are well-suited for development as topical antibacterial drugs. We recently designed a synthetic linear 4-residue peptide, BRBR-NH2, with potent bactericidal activity against Staphylococcus aureus (MIC 6.25 μM), the main ca...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Online Access: | https://hdl.handle.net/10356/90094 http://hdl.handle.net/10220/48405 |
| _version_ | 1826110925120208896 |
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| author | Lau, Qiu Ying Li, Jianguo Sani, Marc-Antoine Sinha, Sheetal Li, Yan Ng, Fui Mee Kang, CongBao Bhattacharjya, Surajit Separovic, Frances Verma, Chandra Chia, Brian Cheng San |
| author2 | Interdisciplinary Graduate School (IGS) |
| author_facet | Interdisciplinary Graduate School (IGS) Lau, Qiu Ying Li, Jianguo Sani, Marc-Antoine Sinha, Sheetal Li, Yan Ng, Fui Mee Kang, CongBao Bhattacharjya, Surajit Separovic, Frances Verma, Chandra Chia, Brian Cheng San |
| author_sort | Lau, Qiu Ying |
| collection | NTU |
| description | Linear antimicrobial peptides, with their rapid bactericidal mode of action, are well-suited for development as topical antibacterial drugs. We recently designed a synthetic linear 4-residue peptide, BRBR-NH2, with potent bactericidal activity against Staphylococcus aureus (MIC 6.25 μM), the main causative pathogen of human skin infections with an unknown mechanism of action. Herein, we describe a series of experiments conducted to gain further insights into its mechanism of action involving electron microscopy, artificial membrane dye leakage, solution- and solid-state NMR spectroscopy followed by molecular dynamics simulations. Experimental results point towards a SMART (Soft Membranes Adapt and Respond, also Transiently) mechanism of action, suggesting that the peptide can be developed as a topical antibacterial agent for treating drug-resistant Staphylococcus aureus infections. |
| first_indexed | 2024-10-01T02:42:09Z |
| format | Journal Article |
| id | ntu-10356/90094 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:42:09Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/900942023-02-28T17:03:03Z Elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide BRBR-NH 2 Lau, Qiu Ying Li, Jianguo Sani, Marc-Antoine Sinha, Sheetal Li, Yan Ng, Fui Mee Kang, CongBao Bhattacharjya, Surajit Separovic, Frances Verma, Chandra Chia, Brian Cheng San Interdisciplinary Graduate School (IGS) School of Biological Sciences Advanced Environmental Biotechnology Centre Nanyang Environment and Water Research Institute Antimicrobial Peptide Membrane Disruptor DRNTU::Science::Biological sciences Linear antimicrobial peptides, with their rapid bactericidal mode of action, are well-suited for development as topical antibacterial drugs. We recently designed a synthetic linear 4-residue peptide, BRBR-NH2, with potent bactericidal activity against Staphylococcus aureus (MIC 6.25 μM), the main causative pathogen of human skin infections with an unknown mechanism of action. Herein, we describe a series of experiments conducted to gain further insights into its mechanism of action involving electron microscopy, artificial membrane dye leakage, solution- and solid-state NMR spectroscopy followed by molecular dynamics simulations. Experimental results point towards a SMART (Soft Membranes Adapt and Respond, also Transiently) mechanism of action, suggesting that the peptide can be developed as a topical antibacterial agent for treating drug-resistant Staphylococcus aureus infections. ASTAR (Agency for Sci., Tech. and Research, S’pore) MOE (Min. of Education, S’pore) Accepted version 2019-05-28T04:16:00Z 2019-12-06T17:40:32Z 2019-05-28T04:16:00Z 2019-12-06T17:40:32Z 2018 Journal Article Lau, Q. Y., Li, J., Sani, M.-A., Sinha, S., Li, Y., Ng, F. M., . . . Chia, C. S. B. (2018). Elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide BRBR-NH 2. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1860(8), 1517-1527. doi:10.1016/j.bbamem.2018.05.004 0005-2736 https://hdl.handle.net/10356/90094 http://hdl.handle.net/10220/48405 10.1016/j.bbamem.2018.05.004 en Biochimica et Biophysica Acta (BBA) - Biomembranes © 2018 Elsevier B.V. All rights reserved. This paper was published in Biochimica et Biophysica Acta (BBA) - Biomembranes and is made available with permission of Elsevier B.V. 41 p. application/pdf |
| spellingShingle | Antimicrobial Peptide Membrane Disruptor DRNTU::Science::Biological sciences Lau, Qiu Ying Li, Jianguo Sani, Marc-Antoine Sinha, Sheetal Li, Yan Ng, Fui Mee Kang, CongBao Bhattacharjya, Surajit Separovic, Frances Verma, Chandra Chia, Brian Cheng San Elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide BRBR-NH 2 |
| title | Elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide BRBR-NH 2 |
| title_full | Elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide BRBR-NH 2 |
| title_fullStr | Elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide BRBR-NH 2 |
| title_full_unstemmed | Elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide BRBR-NH 2 |
| title_short | Elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide BRBR-NH 2 |
| title_sort | elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide brbr nh 2 |
| topic | Antimicrobial Peptide Membrane Disruptor DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/90094 http://hdl.handle.net/10220/48405 |
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