Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae)
Crassulacean acid metabolism (CAM) photosynthesis is a modification of the core C3 photosynthetic pathway that improves the ability of plants to assimilate carbon in water-limited environments. CAM plants fix CO2 mostly at night, when transpiration rates are low. All of the CAM pathway genes exist i...
| Hlavní autoři: | , , , , , , , , , |
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| Médium: | Journal Article |
| Jazyk: | English |
| Vydáno: |
2019
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| On-line přístup: | https://hdl.handle.net/10356/90254 http://hdl.handle.net/10220/48461 |
| _version_ | 1826125030968262656 |
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| author | Winter, Klaus Leebens-Mack, Jim Heyduk, Karolina Hwang, Michelle Albert, Victor Silvera, Katia Lan, Tianying Farr, Kimberly Chang, Tien-Hao Chan, Ming-Tsair |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Winter, Klaus Leebens-Mack, Jim Heyduk, Karolina Hwang, Michelle Albert, Victor Silvera, Katia Lan, Tianying Farr, Kimberly Chang, Tien-Hao Chan, Ming-Tsair |
| author_sort | Winter, Klaus |
| collection | NTU |
| description | Crassulacean acid metabolism (CAM) photosynthesis is a modification of the core C3 photosynthetic pathway that improves the ability of plants to assimilate carbon in water-limited environments. CAM plants fix CO2 mostly at night, when transpiration rates are low. All of the CAM pathway genes exist in ancestral C3 species, but the timing and magnitude of expression are greatly altered between C3 and CAM species. Understanding these regulatory changes is key to elucidating the mechanism by which CAM evolved from C3. Here, we use two closely related species in the Orchidaceae, Erycina pusilla (CAM) and Erycina crista-galli (C3), to conduct comparative transcriptomic analyses across multiple time points. Clustering of genes with expression variation across the diel cycle revealed some canonical CAM pathway genes similarly expressed in both species, regardless of photosynthetic pathway. However, gene network construction indicated that 149 gene families had significant differences in network connectivity and were further explored for these functional enrichments. Genes involved in light sensing and ABA signaling were some of the most differently connected genes between the C3 and CAM Erycina species, in agreement with the contrasting diel patterns of stomatal conductance in C3 and CAM plants. Our results suggest changes to transcriptional cascades are important for the transition from C3 to CAM photosynthesis in Erycina. |
| first_indexed | 2024-10-01T06:30:04Z |
| format | Journal Article |
| id | ntu-10356/90254 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:30:04Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/902542023-02-28T17:03:10Z Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae) Winter, Klaus Leebens-Mack, Jim Heyduk, Karolina Hwang, Michelle Albert, Victor Silvera, Katia Lan, Tianying Farr, Kimberly Chang, Tien-Hao Chan, Ming-Tsair School of Biological Sciences RNA-seq Transcriptomics DRNTU::Science::Biological sciences Crassulacean acid metabolism (CAM) photosynthesis is a modification of the core C3 photosynthetic pathway that improves the ability of plants to assimilate carbon in water-limited environments. CAM plants fix CO2 mostly at night, when transpiration rates are low. All of the CAM pathway genes exist in ancestral C3 species, but the timing and magnitude of expression are greatly altered between C3 and CAM species. Understanding these regulatory changes is key to elucidating the mechanism by which CAM evolved from C3. Here, we use two closely related species in the Orchidaceae, Erycina pusilla (CAM) and Erycina crista-galli (C3), to conduct comparative transcriptomic analyses across multiple time points. Clustering of genes with expression variation across the diel cycle revealed some canonical CAM pathway genes similarly expressed in both species, regardless of photosynthetic pathway. However, gene network construction indicated that 149 gene families had significant differences in network connectivity and were further explored for these functional enrichments. Genes involved in light sensing and ABA signaling were some of the most differently connected genes between the C3 and CAM Erycina species, in agreement with the contrasting diel patterns of stomatal conductance in C3 and CAM plants. Our results suggest changes to transcriptional cascades are important for the transition from C3 to CAM photosynthesis in Erycina. Published version 2019-05-29T08:35:55Z 2019-12-06T17:44:07Z 2019-05-29T08:35:55Z 2019-12-06T17:44:07Z 2019 Journal Article Heyduk, K., Hwang, M., Albert, V., Silvera, K., Lan, T., Farr, K., . . . Leebens-Mack, J. (2019). Altered Gene Regulatory Networks Are Associated With the Transition From C3 to Crassulacean Acid Metabolism in Erycina (Oncidiinae: Orchidaceae). Frontiers in Plant Science, 9, 2000-. doi:10.3389/fpls.2018.02000 https://hdl.handle.net/10356/90254 http://hdl.handle.net/10220/48461 10.3389/fpls.2018.02000 en Frontiers in Plant Science © 2019 Heyduk, Hwang, Albert, Silvera, Lan, Farr, Chang, Chan, Winter and Leebens-Mack. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 15 p. application/pdf |
| spellingShingle | RNA-seq Transcriptomics DRNTU::Science::Biological sciences Winter, Klaus Leebens-Mack, Jim Heyduk, Karolina Hwang, Michelle Albert, Victor Silvera, Katia Lan, Tianying Farr, Kimberly Chang, Tien-Hao Chan, Ming-Tsair Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae) |
| title | Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae) |
| title_full | Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae) |
| title_fullStr | Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae) |
| title_full_unstemmed | Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae) |
| title_short | Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae) |
| title_sort | altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina oncidiinae orchidaceae |
| topic | RNA-seq Transcriptomics DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/90254 http://hdl.handle.net/10220/48461 |
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