Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature
Photoacoustic (PA) tomography (PAT) imaging is an emerging biomedical imaging modality useful in various preclinical and clinical applications. Custom-made circular ring array-based transducers and conventional bulky Nd:YAG/OPO lasers inhibit translation of the PAT system to clinics. Ultra-compact...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Online Access: | https://hdl.handle.net/10356/91257 http://hdl.handle.net/10220/48791 |
| _version_ | 1826123758484586496 |
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| author | Kalva, Sandeep Kumar Upputuri, Paul Kumar Rajendran, Praveenbalaji Dienzo, Rhonnie Austria Pramanik, Manojit |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Kalva, Sandeep Kumar Upputuri, Paul Kumar Rajendran, Praveenbalaji Dienzo, Rhonnie Austria Pramanik, Manojit |
| author_sort | Kalva, Sandeep Kumar |
| collection | NTU |
| description | Photoacoustic (PA) tomography (PAT) imaging is an emerging biomedical imaging modality useful in various preclinical and clinical applications. Custom-made circular ring array-based transducers and conventional bulky Nd:YAG/OPO lasers inhibit translation of the PAT system to clinics.
Ultra-compact pulsed laser diodes (PLDs) are currently being used as an alternative source of near-infrared excitation for PA imaging. Highspeed dynamic in vivo imaging has been demonstrated using a compact PLD-based desktop PAT system (PLD-PAT). A visualized experimental
protocol using the desktop PLD-PAT system is provided in this work for dynamic in vivo brain imaging. The protocol describes the desktop PLDPAT system configuration, preparation of animal for brain vascular imaging, and procedure for dynamic visualization of indocyanine green (ICG)
dye uptake and clearance process in rat cortical vasculature. |
| first_indexed | 2024-10-01T06:09:34Z |
| format | Journal Article |
| id | ntu-10356/91257 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:09:34Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/912572023-12-29T06:50:23Z Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature Kalva, Sandeep Kumar Upputuri, Paul Kumar Rajendran, Praveenbalaji Dienzo, Rhonnie Austria Pramanik, Manojit School of Chemical and Biomedical Engineering Acoustic Reflector Bioengineering DRNTU::Engineering::Chemical engineering Photoacoustic (PA) tomography (PAT) imaging is an emerging biomedical imaging modality useful in various preclinical and clinical applications. Custom-made circular ring array-based transducers and conventional bulky Nd:YAG/OPO lasers inhibit translation of the PAT system to clinics. Ultra-compact pulsed laser diodes (PLDs) are currently being used as an alternative source of near-infrared excitation for PA imaging. Highspeed dynamic in vivo imaging has been demonstrated using a compact PLD-based desktop PAT system (PLD-PAT). A visualized experimental protocol using the desktop PLD-PAT system is provided in this work for dynamic in vivo brain imaging. The protocol describes the desktop PLDPAT system configuration, preparation of animal for brain vascular imaging, and procedure for dynamic visualization of indocyanine green (ICG) dye uptake and clearance process in rat cortical vasculature. NMRC (Natl Medical Research Council, S’pore) Published version 2019-06-18T03:14:35Z 2019-12-06T18:02:27Z 2019-06-18T03:14:35Z 2019-12-06T18:02:27Z 2019 2019 Journal Article Kalva, S. K., Upputuri, P. K., Rajendran, P., Dienzo, R. A., & Pramanik, M. (2019). Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature. Journal of Visualized Experiments, (147). doi:10.3791/59764 https://hdl.handle.net/10356/91257 http://hdl.handle.net/10220/48791 10.3791/59764 214584 en Journal of Visualized Experiments © 2019 The Author(s) (Journal of Visualized Experiments). All rights reserved. This paper was published in Journal of Visualized Experiments and is made available with permission of The Author(s) (Journal of Visualized Experiments). 6 p. application/pdf |
| spellingShingle | Acoustic Reflector Bioengineering DRNTU::Engineering::Chemical engineering Kalva, Sandeep Kumar Upputuri, Paul Kumar Rajendran, Praveenbalaji Dienzo, Rhonnie Austria Pramanik, Manojit Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature |
| title | Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature |
| title_full | Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature |
| title_fullStr | Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature |
| title_full_unstemmed | Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature |
| title_short | Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature |
| title_sort | pulsed laser diode based desktop photoacoustic tomography for monitoring wash in and wash out of dye in rat cortical vasculature |
| topic | Acoustic Reflector Bioengineering DRNTU::Engineering::Chemical engineering |
| url | https://hdl.handle.net/10356/91257 http://hdl.handle.net/10220/48791 |
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