Differences in erythrocyte receptor specificity of different parts of the plasmodium falciparum reticulocyte binding protein homologue 2a
The Plasmodium falciparum reticulocyte-binding-like protein homologue (RH) and erythrocyte binding-like (EBL) protein families play important roles during invasion, though their exact roles are not clear. Both EBL and RH proteins are thought to directly bind different receptors on the surface of the...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/93872 http://hdl.handle.net/10220/7305 |
| _version_ | 1826126914131066880 |
|---|---|
| author | Preiser, Peter Rainer Karthigayan Gunalan Gao, Xiaohong Liew, Kingsley Jiin Liang |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Preiser, Peter Rainer Karthigayan Gunalan Gao, Xiaohong Liew, Kingsley Jiin Liang |
| author_sort | Preiser, Peter Rainer |
| collection | NTU |
| description | The Plasmodium falciparum reticulocyte-binding-like protein homologue (RH) and erythrocyte binding-like (EBL) protein families play important roles during invasion, though their exact roles are not clear. Both EBL and RH proteins are thought to directly bind different receptors on the surface of the erythrocyte, and the binding properties for a number of EBLs and RHs have been described. While P. falciparum RH1 (PfRH1) and PfRH4 have been shown to act directly in two alternative invasion pathways used by merozoites, the functions of PfRH2a and PfRH2b during invasion are less defined. Here, using monoclonal antibodies raised against a unique region of PfRH2a, we show that PfRH2a moves from the rhoptry neck to the moving junction during merozoite invasion. The movement of PfRH2a to the junction is independent of the invasion pathway used by the merozoite, suggesting an additional function of the protein that is independent of receptor binding. We further show that PfRH2a is processed both in the schizont and during invasion, resulting in proteins with different erythrocyte binding properties. Our findings suggest that PfRH2a and, most likely, the other members of the RH family, depending on their processing stage, can engage different receptors at different stages of the invasion process. |
| first_indexed | 2024-10-01T07:00:12Z |
| format | Journal Article |
| id | ntu-10356/93872 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:00:12Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/938722023-02-28T17:02:00Z Differences in erythrocyte receptor specificity of different parts of the plasmodium falciparum reticulocyte binding protein homologue 2a Preiser, Peter Rainer Karthigayan Gunalan Gao, Xiaohong Liew, Kingsley Jiin Liang School of Biological Sciences A*STAR Biomedical Research Council DRNTU::Science::Biological sciences::Microbiology::Virology The Plasmodium falciparum reticulocyte-binding-like protein homologue (RH) and erythrocyte binding-like (EBL) protein families play important roles during invasion, though their exact roles are not clear. Both EBL and RH proteins are thought to directly bind different receptors on the surface of the erythrocyte, and the binding properties for a number of EBLs and RHs have been described. While P. falciparum RH1 (PfRH1) and PfRH4 have been shown to act directly in two alternative invasion pathways used by merozoites, the functions of PfRH2a and PfRH2b during invasion are less defined. Here, using monoclonal antibodies raised against a unique region of PfRH2a, we show that PfRH2a moves from the rhoptry neck to the moving junction during merozoite invasion. The movement of PfRH2a to the junction is independent of the invasion pathway used by the merozoite, suggesting an additional function of the protein that is independent of receptor binding. We further show that PfRH2a is processed both in the schizont and during invasion, resulting in proteins with different erythrocyte binding properties. Our findings suggest that PfRH2a and, most likely, the other members of the RH family, depending on their processing stage, can engage different receptors at different stages of the invasion process. Accepted version 2011-10-19T00:51:28Z 2019-12-06T18:46:56Z 2011-10-19T00:51:28Z 2019-12-06T18:46:56Z 2011 2011 Journal Article Karthigayan, G., Gao, X., Liew, K. J. L., & Preiser, P. R. (2011). Differences in Erythrocyte Receptor Specificity of Different Parts of the Plasmodium falciparum Reticulocyte Binding Protein Homologue 2a. Infection and Immunity, 79(8), 3421-3430. 0019-9567 https://hdl.handle.net/10356/93872 http://hdl.handle.net/10220/7305 10.1128/IAI.00201-11 21628513 161135 en Infection and immunity © 2011 American Society for Microbiology. This is the author created version of a work that has been peer reviewed and accepted for publication by Infection and Immunity, American Society for Microbiology. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI: http://dx.doi.org/10.1128/IAI.00201-11] 10 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Virology Preiser, Peter Rainer Karthigayan Gunalan Gao, Xiaohong Liew, Kingsley Jiin Liang Differences in erythrocyte receptor specificity of different parts of the plasmodium falciparum reticulocyte binding protein homologue 2a |
| title | Differences in erythrocyte receptor specificity of different parts of the plasmodium falciparum reticulocyte binding protein homologue 2a |
| title_full | Differences in erythrocyte receptor specificity of different parts of the plasmodium falciparum reticulocyte binding protein homologue 2a |
| title_fullStr | Differences in erythrocyte receptor specificity of different parts of the plasmodium falciparum reticulocyte binding protein homologue 2a |
| title_full_unstemmed | Differences in erythrocyte receptor specificity of different parts of the plasmodium falciparum reticulocyte binding protein homologue 2a |
| title_short | Differences in erythrocyte receptor specificity of different parts of the plasmodium falciparum reticulocyte binding protein homologue 2a |
| title_sort | differences in erythrocyte receptor specificity of different parts of the plasmodium falciparum reticulocyte binding protein homologue 2a |
| topic | DRNTU::Science::Biological sciences::Microbiology::Virology |
| url | https://hdl.handle.net/10356/93872 http://hdl.handle.net/10220/7305 |
| work_keys_str_mv | AT preiserpeterrainer differencesinerythrocytereceptorspecificityofdifferentpartsoftheplasmodiumfalciparumreticulocytebindingproteinhomologue2a AT karthigayangunalan differencesinerythrocytereceptorspecificityofdifferentpartsoftheplasmodiumfalciparumreticulocytebindingproteinhomologue2a AT gaoxiaohong differencesinerythrocytereceptorspecificityofdifferentpartsoftheplasmodiumfalciparumreticulocytebindingproteinhomologue2a AT liewkingsleyjiinliang differencesinerythrocytereceptorspecificityofdifferentpartsoftheplasmodiumfalciparumreticulocytebindingproteinhomologue2a |