The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II
The eukaryotic transcription factor TFIIS enhances elongation and nascent transcript cleavage activities of RNA polymerase II in a stalled elongation complex. By site-directed mutagenesis, we have demonstrated that invariant residues Asp-261 and Glu-262 of the nucleic acid-binding TFIIS Zn ribbon ar...
| Autori principali: | , , |
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| Natura: | Conference Paper |
| Lingua: | English |
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2012
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| Accesso online: | https://hdl.handle.net/10356/94050 http://hdl.handle.net/10220/7484 |
| _version_ | 1826117367341514752 |
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| author | Jeon, Choon Ju Yoon, Ho Sup Agarwal, Kan |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Jeon, Choon Ju Yoon, Ho Sup Agarwal, Kan |
| author_sort | Jeon, Choon Ju |
| collection | NTU |
| description | The eukaryotic transcription factor TFIIS enhances elongation and nascent transcript cleavage activities of RNA polymerase II in a stalled elongation complex. By site-directed mutagenesis, we have demonstrated that invariant residues Asp-261 and Glu-262 of the nucleic acid-binding TFIIS Zn ribbon are critical for stimulation of both elongation and RNA cleavage activities of RNA polymerase II. Substitution of either of these residues inactivates both TFIIS functions, suggesting a related role in both activities. These acidic residues may participate in phosphoryl transfer reactions by a two-metal-ion mechanism in a manner analogous to Klenow fragment. The RNA polymerase II itself may contain a Zn ribbon, in as much as the polymerase's 15-kDa subunit contains a sequence that aligns well with the TFIIS Zn ribbon sequence, including a similarly placed pair of acidic residues. |
| first_indexed | 2024-10-01T04:26:27Z |
| format | Conference Paper |
| id | ntu-10356/94050 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:26:27Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/940502023-02-28T16:54:57Z The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II Jeon, Choon Ju Yoon, Ho Sup Agarwal, Kan School of Biological Sciences National Academy of Sciences (1994) DRNTU::Science::Chemistry::Biochemistry The eukaryotic transcription factor TFIIS enhances elongation and nascent transcript cleavage activities of RNA polymerase II in a stalled elongation complex. By site-directed mutagenesis, we have demonstrated that invariant residues Asp-261 and Glu-262 of the nucleic acid-binding TFIIS Zn ribbon are critical for stimulation of both elongation and RNA cleavage activities of RNA polymerase II. Substitution of either of these residues inactivates both TFIIS functions, suggesting a related role in both activities. These acidic residues may participate in phosphoryl transfer reactions by a two-metal-ion mechanism in a manner analogous to Klenow fragment. The RNA polymerase II itself may contain a Zn ribbon, in as much as the polymerase's 15-kDa subunit contains a sequence that aligns well with the TFIIS Zn ribbon sequence, including a similarly placed pair of acidic residues. Accepted version 2012-01-26T08:17:57Z 2019-12-06T18:49:57Z 2012-01-26T08:17:57Z 2019-12-06T18:49:57Z 1994 1994 Conference Paper Jeon, C. J., Yoon, H. S. & Agarwal, K. (1994). The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II. Proceedings of the National Academy of Sciences, pp.9106–9110. https://hdl.handle.net/10356/94050 http://hdl.handle.net/10220/7484 10.1073/pnas.91.19.9106 en © 1994 National Academy of Sciences. This is the author created version of a work that has been peer reviewed and accepted for publication by Proceedings of the National Academy of Sciences , National Academy of Sciences. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1073/pnas.91.19.9106 application/pdf |
| spellingShingle | DRNTU::Science::Chemistry::Biochemistry Jeon, Choon Ju Yoon, Ho Sup Agarwal, Kan The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II |
| title | The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II |
| title_full | The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II |
| title_fullStr | The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II |
| title_full_unstemmed | The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II |
| title_short | The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II |
| title_sort | transcription factor tfiis zinc ribbon dipeptide asp glu is critical for stimulation of elongation and rna cleavage by rna polymerase ii |
| topic | DRNTU::Science::Chemistry::Biochemistry |
| url | https://hdl.handle.net/10356/94050 http://hdl.handle.net/10220/7484 |
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