Digital in-line holography for microscopic applications
Lensless digital holographic microscope (DHM) is presented for the study of transparent micro objects in three dimensions. A highly diverging beam replaces the conventional microscope objectives to provide the desired magnification. Direct evaluation of the phase information from single hologram mak...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Conference Paper |
| Language: | English |
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2011
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| Online Access: | https://hdl.handle.net/10356/94289 http://hdl.handle.net/10220/7192 |
| _version_ | 1811681062925893632 |
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| author | Singh, Vijay Raj Asundi, Anand Krishna |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Singh, Vijay Raj Asundi, Anand Krishna |
| author_sort | Singh, Vijay Raj |
| collection | NTU |
| description | Lensless digital holographic microscope (DHM) is presented for the study of transparent micro objects in three dimensions. A highly diverging beam replaces the conventional microscope objectives to provide the desired magnification. Direct evaluation of the phase information from single hologram makes digital holography a most attractive tool in optical metrology. We successfully implement DHM for the applications of “microholes inspection” and “microfluid analysis”. The amplitude and phase contrast images of microholes are numerically reconstructed and ompared with the conventional optical microscopic images. The direct evaluation of phase information gives the 3-D profile of microholes. Furthermore, the change in the refractive index of microfluid occurred by Brownian motion of particles inside a microchannel is studied. Two digital holograms are recorded corresponding to different states of particles motion, the subtracted phase gives the change in optical path length. |
| first_indexed | 2024-10-01T03:34:59Z |
| format | Conference Paper |
| id | ntu-10356/94289 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:34:59Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/942892019-12-06T18:53:43Z Digital in-line holography for microscopic applications Singh, Vijay Raj Asundi, Anand Krishna School of Mechanical and Aerospace Engineering International Congress of experimental Mechanics (8th : 2005 : New Delhi) DRNTU::Science::Physics::Optics and light Lensless digital holographic microscope (DHM) is presented for the study of transparent micro objects in three dimensions. A highly diverging beam replaces the conventional microscope objectives to provide the desired magnification. Direct evaluation of the phase information from single hologram makes digital holography a most attractive tool in optical metrology. We successfully implement DHM for the applications of “microholes inspection” and “microfluid analysis”. The amplitude and phase contrast images of microholes are numerically reconstructed and ompared with the conventional optical microscopic images. The direct evaluation of phase information gives the 3-D profile of microholes. Furthermore, the change in the refractive index of microfluid occurred by Brownian motion of particles inside a microchannel is studied. Two digital holograms are recorded corresponding to different states of particles motion, the subtracted phase gives the change in optical path length. Accepted version 2011-10-10T06:11:09Z 2019-12-06T18:53:43Z 2011-10-10T06:11:09Z 2019-12-06T18:53:43Z 2005 2005 Conference Paper Singh, V. R., & Asundi, A. K. (2005). Digital in-line holography for microscopic applications. Paper presented at the International Congress of Experimental Mechanics. https://hdl.handle.net/10356/94289 http://hdl.handle.net/10220/7192 143376 en © 2005 International Congress of Experimental Mechanics. 7 p. |
| spellingShingle | DRNTU::Science::Physics::Optics and light Singh, Vijay Raj Asundi, Anand Krishna Digital in-line holography for microscopic applications |
| title | Digital in-line holography for microscopic applications |
| title_full | Digital in-line holography for microscopic applications |
| title_fullStr | Digital in-line holography for microscopic applications |
| title_full_unstemmed | Digital in-line holography for microscopic applications |
| title_short | Digital in-line holography for microscopic applications |
| title_sort | digital in line holography for microscopic applications |
| topic | DRNTU::Science::Physics::Optics and light |
| url | https://hdl.handle.net/10356/94289 http://hdl.handle.net/10220/7192 |
| work_keys_str_mv | AT singhvijayraj digitalinlineholographyformicroscopicapplications AT asundianandkrishna digitalinlineholographyformicroscopicapplications |