Nested PCR in magnetically actuated circular closed-loop PCR microchip system
We demonstrate a new and sensitive amplification technique (referred to as Nested Polymerase Chain Reaction; nPCR). It based on a magnetically actuated circular closed-loop PCR microchip system. nPCR involves the use of two sets of primers in two successive PCR runs, and allows the amplification of...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/94315 http://hdl.handle.net/10220/7905 |
| _version_ | 1826114955706892288 |
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| author | Lok, Khoi Seng Lee, Peter Peng Foo Kwok, Yien Chian Nguyen, Nam-Trung |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Lok, Khoi Seng Lee, Peter Peng Foo Kwok, Yien Chian Nguyen, Nam-Trung |
| author_sort | Lok, Khoi Seng |
| collection | NTU |
| description | We demonstrate a new and sensitive amplification technique (referred to as Nested Polymerase Chain Reaction; nPCR). It based on a magnetically actuated circular closed-loop PCR microchip system. nPCR involves the use of two sets of primers in two successive PCR runs, and allows the amplification of a single locus from a minute quantity of template DNA. Two sets of primers are specially designed to a target 500-bp region of the bacteriophage lambda template DNA in the first PCR run, and a 247-bp region of the targeted 500-bp first PCR product in the second PCR run. PCR is run on the microchip system and concurrently in regular thermocycler for comparison. The products are analyzed by conventional agarose gel electrophoresis. The detection limit for the initial template DNA is 1.63 × 105 copies per μL (or 8.67 pg) for the first PCR run, and 1.63 copies per μL (or 0.0867 fg) for the second run. The results are comparable to a regular thermocycler. This preliminary study opens a new gateway to future development of specialized nPCR on chip. |
| first_indexed | 2024-10-01T03:47:39Z |
| format | Journal Article |
| id | ntu-10356/94315 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:47:39Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/943152023-03-04T17:18:22Z Nested PCR in magnetically actuated circular closed-loop PCR microchip system Lok, Khoi Seng Lee, Peter Peng Foo Kwok, Yien Chian Nguyen, Nam-Trung School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering We demonstrate a new and sensitive amplification technique (referred to as Nested Polymerase Chain Reaction; nPCR). It based on a magnetically actuated circular closed-loop PCR microchip system. nPCR involves the use of two sets of primers in two successive PCR runs, and allows the amplification of a single locus from a minute quantity of template DNA. Two sets of primers are specially designed to a target 500-bp region of the bacteriophage lambda template DNA in the first PCR run, and a 247-bp region of the targeted 500-bp first PCR product in the second PCR run. PCR is run on the microchip system and concurrently in regular thermocycler for comparison. The products are analyzed by conventional agarose gel electrophoresis. The detection limit for the initial template DNA is 1.63 × 105 copies per μL (or 8.67 pg) for the first PCR run, and 1.63 copies per μL (or 0.0867 fg) for the second run. The results are comparable to a regular thermocycler. This preliminary study opens a new gateway to future development of specialized nPCR on chip. 2012-05-11T08:03:32Z 2019-12-06T18:54:02Z 2012-05-11T08:03:32Z 2019-12-06T18:54:02Z 2012 2012 Journal Article Lok, K. S., Lee, P. P. F., Kwok, Y. C., & Nguyen, N. T. (2012). Nested PCR in magnetically actuated circular closed-loop PCR microchip system. Microchimica Acta , 177(1-2), 111-117. https://hdl.handle.net/10356/94315 http://hdl.handle.net/10220/7905 10.1007/s00604-012-0760-2 163867 en Microchimica acta © 2012 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Microchimica acta, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1007/s00604-012-0760-2. 16 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Mechanical engineering Lok, Khoi Seng Lee, Peter Peng Foo Kwok, Yien Chian Nguyen, Nam-Trung Nested PCR in magnetically actuated circular closed-loop PCR microchip system |
| title | Nested PCR in magnetically actuated circular closed-loop PCR microchip system |
| title_full | Nested PCR in magnetically actuated circular closed-loop PCR microchip system |
| title_fullStr | Nested PCR in magnetically actuated circular closed-loop PCR microchip system |
| title_full_unstemmed | Nested PCR in magnetically actuated circular closed-loop PCR microchip system |
| title_short | Nested PCR in magnetically actuated circular closed-loop PCR microchip system |
| title_sort | nested pcr in magnetically actuated circular closed loop pcr microchip system |
| topic | DRNTU::Engineering::Mechanical engineering |
| url | https://hdl.handle.net/10356/94315 http://hdl.handle.net/10220/7905 |
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