Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy
Exocytosis of neurotransmitter or hormone-filled vesicles is a highly dynamic process regulated by various proteins and lipids. As mainly revealed indirectly by the electrophysiological methods, exocytosis is believed to involve multiple kinetic steps in which vesicles tr...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/94319 http://hdl.handle.net/10220/7522 |
| _version_ | 1811697771646812160 |
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| author | Zhang, Enming Xue, Renhao Soo, Jianchow Chen, Peng |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Zhang, Enming Xue, Renhao Soo, Jianchow Chen, Peng |
| author_sort | Zhang, Enming |
| collection | NTU |
| description | Exocytosis of neurotransmitter or hormone-filled
vesicles is a highly dynamic process regulated by various
proteins and lipids. As mainly revealed indirectly by the
electrophysiological methods, exocytosis is believed to
involve multiple kinetic steps in which vesicles transit from
one state to another. Using total internal reflection
fluorescence microscopy which enables direct visualization
of individual vesicles, we developed an analytical framework
to track and analyze vesicle dynamics. We demonstrated
that all subplasmalemmal vesicles generally undergo
constant and caged Brownian motion. And they can be
classified into three populations that differ in their motion
characteristics and fusion competence. Furthermore, we
showed that these vesicle pools are differentially modulated
by phorbol-12-myristate-13-acetate, a phorbol ester analog
to endogenous diacylglycerol, through both protein-kinase-
C-dependent and -independent pathways. |
| first_indexed | 2024-10-01T08:00:33Z |
| format | Journal Article |
| id | ntu-10356/94319 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T08:00:33Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/943192023-12-29T06:53:08Z Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy Zhang, Enming Xue, Renhao Soo, Jianchow Chen, Peng School of Chemical and Biomedical Engineering DRNTU::Science::Medicine::Biomedical engineering Exocytosis of neurotransmitter or hormone-filled vesicles is a highly dynamic process regulated by various proteins and lipids. As mainly revealed indirectly by the electrophysiological methods, exocytosis is believed to involve multiple kinetic steps in which vesicles transit from one state to another. Using total internal reflection fluorescence microscopy which enables direct visualization of individual vesicles, we developed an analytical framework to track and analyze vesicle dynamics. We demonstrated that all subplasmalemmal vesicles generally undergo constant and caged Brownian motion. And they can be classified into three populations that differ in their motion characteristics and fusion competence. Furthermore, we showed that these vesicle pools are differentially modulated by phorbol-12-myristate-13-acetate, a phorbol ester analog to endogenous diacylglycerol, through both protein-kinase- C-dependent and -independent pathways. Accepted version 2012-02-14T07:13:45Z 2019-12-06T18:54:06Z 2012-02-14T07:13:45Z 2019-12-06T18:54:06Z 2008 2008 Journal Article Zhang, E., Xue, R., Soo, J. & Chen, P. (2008). Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy. Pflügers Archiv European Journal of Physiology, 457, 211-222. https://hdl.handle.net/10356/94319 http://hdl.handle.net/10220/7522 10.1007/s00424-008-0485-6 118348 en Pflügers archiv European journal of physiology © 2008 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Pflügers Archiv European Journal of Physiology, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI: http://dx.doi.org/10.1007/s00424-008-0485-6] application/pdf |
| spellingShingle | DRNTU::Science::Medicine::Biomedical engineering Zhang, Enming Xue, Renhao Soo, Jianchow Chen, Peng Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy |
| title | Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy |
| title_full | Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy |
| title_fullStr | Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy |
| title_full_unstemmed | Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy |
| title_short | Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy |
| title_sort | effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy |
| topic | DRNTU::Science::Medicine::Biomedical engineering |
| url | https://hdl.handle.net/10356/94319 http://hdl.handle.net/10220/7522 |
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