Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots
It has been increasingly recognized that adenosine-5 ′ -triphosphate (ATP) signaling through P2 purinergic receptors has a diverse infl uence on cell functions and human health. In this contribution, a simple and non-invasive method to specifi cally label P2 receptors using ATP-...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/94388 http://hdl.handle.net/10220/7492 |
| _version_ | 1826119167671009280 |
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| author | Jiang, Shan Liu, Aiping Duan, Hongwei Soo, Jianchow Chen, Peng |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Jiang, Shan Liu, Aiping Duan, Hongwei Soo, Jianchow Chen, Peng |
| author_sort | Jiang, Shan |
| collection | NTU |
| description | It has been increasingly recognized that adenosine-5 ′ -triphosphate (ATP)
signaling through P2 purinergic receptors has a diverse infl uence on cell
functions and human health. In this contribution, a simple and non-invasive
method to specifi cally label P2 receptors using ATP-conjugated quantum dots
(QDs) is demonstrated. In addition, the endocytosis, vesicular traffi cking,
and recycling of QD-labeled P2 receptors are studied under different physiological
conditions using confocal and total internal refl ection fl uorescence
microscopy. |
| first_indexed | 2024-10-01T04:55:41Z |
| format | Journal Article |
| id | ntu-10356/94388 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:55:41Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/943882020-03-07T11:35:34Z Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots Jiang, Shan Liu, Aiping Duan, Hongwei Soo, Jianchow Chen, Peng School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering It has been increasingly recognized that adenosine-5 ′ -triphosphate (ATP) signaling through P2 purinergic receptors has a diverse infl uence on cell functions and human health. In this contribution, a simple and non-invasive method to specifi cally label P2 receptors using ATP-conjugated quantum dots (QDs) is demonstrated. In addition, the endocytosis, vesicular traffi cking, and recycling of QD-labeled P2 receptors are studied under different physiological conditions using confocal and total internal refl ection fl uorescence microscopy. 2012-02-02T05:26:46Z 2019-12-06T18:55:20Z 2012-02-02T05:26:46Z 2019-12-06T18:55:20Z 2011 2011 Journal Article Jiang, S., Liu, A., Duan, H., Soo, J., & Chen, P. (2011). Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots. Advanced Functional Materials, 21, 2776–2780. 1616-301X https://hdl.handle.net/10356/94388 http://hdl.handle.net/10220/7492 10.1002/adfm.201100472 163176 en Advanced functional materials © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim 5 p. |
| spellingShingle | DRNTU::Engineering::Chemical engineering Jiang, Shan Liu, Aiping Duan, Hongwei Soo, Jianchow Chen, Peng Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots |
| title | Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots |
| title_full | Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots |
| title_fullStr | Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots |
| title_full_unstemmed | Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots |
| title_short | Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots |
| title_sort | labeling and tracking p2 purinergic receptors in living cells using atp conjugated quantum dots |
| topic | DRNTU::Engineering::Chemical engineering |
| url | https://hdl.handle.net/10356/94388 http://hdl.handle.net/10220/7492 |
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