Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis
The subunit B of cholera toxin (CTB), which specifically binds with ganglioside GM1 enriched in membrane lipid rafts, is known to interfere with multiple cell functions. However, the specific, stable and spatially defined membrane signaling induced by CTB binding is often difficult to inves...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/94544 http://hdl.handle.net/10220/8406 |
| _version_ | 1826113840290463744 |
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| author | Soo, Jianchow Zhang, Jing He, Qiyuan Agarwal, Shuchi Li, Hai Zhang, Hua Chen, Peng |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Soo, Jianchow Zhang, Jing He, Qiyuan Agarwal, Shuchi Li, Hai Zhang, Hua Chen, Peng |
| author_sort | Soo, Jianchow |
| collection | NTU |
| description | The subunit B of cholera toxin (CTB), which specifically binds with ganglioside GM1 enriched
in membrane lipid rafts, is known to interfere with multiple cell functions. However, the specific,
stable and spatially defined membrane signaling induced by CTB binding is often difficult to
investigate by applying CTB molecules in bulk solution due to quick internalization, elicited
intracellular reactions, and homogeneous interaction with the entire cell membrane. Here, we
interfaced the neuroendocrine PC12 cells with surface immobilized and patterned CTB molecules,
and interrogated the effects of CTB binding on vesicular exocytosis using integrative single-cell
study methods. It was discovered that CTB binding facilitates vesicle trafficking, docking and
exocytosis in a cholesterol dependent manner. And these effects are probably attributable to the
increased membrane GM1 and cholesterol, and enhanced Ca2+ signaling. |
| first_indexed | 2024-10-01T03:29:49Z |
| format | Journal Article |
| id | ntu-10356/94544 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:29:49Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/945442023-07-14T15:58:51Z Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis Soo, Jianchow Zhang, Jing He, Qiyuan Agarwal, Shuchi Li, Hai Zhang, Hua Chen, Peng School of Materials Science & Engineering DRNTU::Science::Biological sciences The subunit B of cholera toxin (CTB), which specifically binds with ganglioside GM1 enriched in membrane lipid rafts, is known to interfere with multiple cell functions. However, the specific, stable and spatially defined membrane signaling induced by CTB binding is often difficult to investigate by applying CTB molecules in bulk solution due to quick internalization, elicited intracellular reactions, and homogeneous interaction with the entire cell membrane. Here, we interfaced the neuroendocrine PC12 cells with surface immobilized and patterned CTB molecules, and interrogated the effects of CTB binding on vesicular exocytosis using integrative single-cell study methods. It was discovered that CTB binding facilitates vesicle trafficking, docking and exocytosis in a cholesterol dependent manner. And these effects are probably attributable to the increased membrane GM1 and cholesterol, and enhanced Ca2+ signaling. Accepted version 2012-08-21T05:51:03Z 2019-12-06T18:57:55Z 2012-08-21T05:51:03Z 2019-12-06T18:57:55Z 2010 2010 Journal Article Soo, J. C., Zhang, J., He, Q., Agarwal, S., Li, H., Zhang, H., et al. (2010). Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis. Integrative Biology, 2, 250–257. https://hdl.handle.net/10356/94544 http://hdl.handle.net/10220/8406 10.1039/c0ib00006j 151699 en Integrative biology © 2010 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Integrative Biology, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1039/c0ib00006j. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Soo, Jianchow Zhang, Jing He, Qiyuan Agarwal, Shuchi Li, Hai Zhang, Hua Chen, Peng Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis |
| title | Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis |
| title_full | Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis |
| title_fullStr | Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis |
| title_full_unstemmed | Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis |
| title_short | Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis |
| title_sort | surface immobilized cholera toxin b subunit ctb facilitates vesicle docking trafficking and exocytosis |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/94544 http://hdl.handle.net/10220/8406 |
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