Total-internal-reflection-fluorescence microscopy for the study of nanobubble dynamics
Nanobubbles can be observed with optical microscopy using the total-internal-reflection-fluorescence excitation. We report on total-internal-reflection-fluorescence visualization using rhodamine 6G at 5 μM concentration which results in strongly contrasting pictures. The preferential absorption and...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/94918 http://hdl.handle.net/10220/9264 |
| _version_ | 1811693928785641472 |
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| author | Chan, Chon U. Ohl, Claus-Dieter |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Chan, Chon U. Ohl, Claus-Dieter |
| author_sort | Chan, Chon U. |
| collection | NTU |
| description | Nanobubbles can be observed with optical microscopy using the total-internal-reflection-fluorescence excitation. We report on total-internal-reflection-fluorescence visualization using rhodamine 6G at 5 μM concentration which results in strongly contrasting pictures. The preferential absorption and the high spatial resolution allow us to detect nanobubbles with diameters of 230 nm and above. We resolve the nucleation dynamics during the water-ethanol-water exchange: within 4 min after exchange the bubbles nucleate and form a stable population. Additionally, we demonstrate that tracer particles near to the nanobubbles are following Brownian motion: the remaining drift flow is weaker than a few micrometers per second at a distance of 400 nm from the nanobubble’s center. |
| first_indexed | 2024-10-01T06:59:29Z |
| format | Journal Article |
| id | ntu-10356/94918 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:59:29Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/949182023-02-28T19:38:27Z Total-internal-reflection-fluorescence microscopy for the study of nanobubble dynamics Chan, Chon U. Ohl, Claus-Dieter School of Physical and Mathematical Sciences DRNTU::Science::Physics Nanobubbles can be observed with optical microscopy using the total-internal-reflection-fluorescence excitation. We report on total-internal-reflection-fluorescence visualization using rhodamine 6G at 5 μM concentration which results in strongly contrasting pictures. The preferential absorption and the high spatial resolution allow us to detect nanobubbles with diameters of 230 nm and above. We resolve the nucleation dynamics during the water-ethanol-water exchange: within 4 min after exchange the bubbles nucleate and form a stable population. Additionally, we demonstrate that tracer particles near to the nanobubbles are following Brownian motion: the remaining drift flow is weaker than a few micrometers per second at a distance of 400 nm from the nanobubble’s center. Published version 2013-02-27T02:04:23Z 2019-12-06T19:04:40Z 2013-02-27T02:04:23Z 2019-12-06T19:04:40Z 2012 2012 Journal Article Chan, C. U., & Ohl, C.- D. (2012). Total-Internal-Reflection-Fluorescence Microscopy for the Study of Nanobubble Dynamics. Physical Review Letters, 109(17), 174501. https://hdl.handle.net/10356/94918 http://hdl.handle.net/10220/9264 10.1103/PhysRevLett.109.174501 en Physical review letters © 2012 American Physical Society. This paper was published in Physical Review Letters and is made available as an electronic reprint (preprint) with permission of American Physical Society. The paper can be found at the following official DOI: [http://dx.doi.org/10.1103/PhysRevLett.109.174501]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf |
| spellingShingle | DRNTU::Science::Physics Chan, Chon U. Ohl, Claus-Dieter Total-internal-reflection-fluorescence microscopy for the study of nanobubble dynamics |
| title | Total-internal-reflection-fluorescence microscopy for the study of nanobubble dynamics |
| title_full | Total-internal-reflection-fluorescence microscopy for the study of nanobubble dynamics |
| title_fullStr | Total-internal-reflection-fluorescence microscopy for the study of nanobubble dynamics |
| title_full_unstemmed | Total-internal-reflection-fluorescence microscopy for the study of nanobubble dynamics |
| title_short | Total-internal-reflection-fluorescence microscopy for the study of nanobubble dynamics |
| title_sort | total internal reflection fluorescence microscopy for the study of nanobubble dynamics |
| topic | DRNTU::Science::Physics |
| url | https://hdl.handle.net/10356/94918 http://hdl.handle.net/10220/9264 |
| work_keys_str_mv | AT chanchonu totalinternalreflectionfluorescencemicroscopyforthestudyofnanobubbledynamics AT ohlclausdieter totalinternalreflectionfluorescencemicroscopyforthestudyofnanobubbledynamics |