In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase
Under the fluctuating circumstances provided by the innate dynamics of microtubules and opposing tensions resulted from microtubule-associated motors, it is vital to ensure stable kinetochore-microtubule attachments for accurate segregation. However, a comprehensive understanding of how this regulat...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/95148 http://hdl.handle.net/10220/9210 |
| _version_ | 1811680575445008384 |
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| author | Lee, Yoke Peng Wong, Chi Hang Chan, Kheng Sze Li, Hoi Yeung Koh, Cheng Gee Lai, Soak Kuan |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Lee, Yoke Peng Wong, Chi Hang Chan, Kheng Sze Li, Hoi Yeung Koh, Cheng Gee Lai, Soak Kuan |
| author_sort | Lee, Yoke Peng |
| collection | NTU |
| description | Under the fluctuating circumstances provided by the innate dynamics of microtubules and opposing tensions resulted from microtubule-associated motors, it is vital to ensure stable kinetochore-microtubule attachments for accurate segregation. However, a comprehensive understanding of how this regulation is mechanistically achieved remains elusive. Using our newly designed live cell FRET time-lapse imaging, we found that post-metaphase RanGTP is crucial in the maintenance of stable kinetochore-microtubule attachments by regulating Aurora B kinase via the NES-bearing Mst1. More importantly, our study demonstrates that by ensuring stable alignment of metaphase chromosomes prior to segregation, RanGTP is indispensible in governing the genomic integrity and the fidelity of cell cycle progression. Our findings suggest an additional role of RanGTP beyond its known function in mitotic spindle assembly during the prometaphase-metaphase transition. |
| first_indexed | 2024-10-01T03:27:14Z |
| format | Journal Article |
| id | ntu-10356/95148 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:27:14Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/951482023-02-28T17:03:55Z In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase Lee, Yoke Peng Wong, Chi Hang Chan, Kheng Sze Li, Hoi Yeung Koh, Cheng Gee Lai, Soak Kuan School of Biological Sciences Under the fluctuating circumstances provided by the innate dynamics of microtubules and opposing tensions resulted from microtubule-associated motors, it is vital to ensure stable kinetochore-microtubule attachments for accurate segregation. However, a comprehensive understanding of how this regulation is mechanistically achieved remains elusive. Using our newly designed live cell FRET time-lapse imaging, we found that post-metaphase RanGTP is crucial in the maintenance of stable kinetochore-microtubule attachments by regulating Aurora B kinase via the NES-bearing Mst1. More importantly, our study demonstrates that by ensuring stable alignment of metaphase chromosomes prior to segregation, RanGTP is indispensible in governing the genomic integrity and the fidelity of cell cycle progression. Our findings suggest an additional role of RanGTP beyond its known function in mitotic spindle assembly during the prometaphase-metaphase transition. Published version 2013-02-20T08:13:52Z 2019-12-06T19:09:13Z 2013-02-20T08:13:52Z 2019-12-06T19:09:13Z 2012 2012 Journal Article Lee, Y.-P., Wong, C.-H., Chan, K.-S., Lai, S.-K., Koh, C.-G., & Li, H.-Y. (2012). In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase. PLoS ONE, 7(9). 1932-6203 https://hdl.handle.net/10356/95148 http://hdl.handle.net/10220/9210 10.1371/journal.pone.0045836 23029267 en PLoS ONE © 2012 The Authors. application/pdf |
| spellingShingle | Lee, Yoke Peng Wong, Chi Hang Chan, Kheng Sze Li, Hoi Yeung Koh, Cheng Gee Lai, Soak Kuan In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase |
| title | In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase |
| title_full | In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase |
| title_fullStr | In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase |
| title_full_unstemmed | In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase |
| title_short | In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase |
| title_sort | in vivo fret imaging revealed a regulatory role of rangtp in kinetochore microtubule attachments via aurora b kinase |
| url | https://hdl.handle.net/10356/95148 http://hdl.handle.net/10220/9210 |
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