Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae

Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae

V-ATPases are very complex multi-subunit enzymes which function as proton-pumping rotary nanomotors. The rotary and coupling subunit F (F1-94) was crystallized by the hanging-drop vapour-diffusion method. The native crystals diffracted to a resolution of 2.64 Å and belonged to space group C2221, wit...

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Bibliographic Details
Main Authors:	Basak, Sandip, Balakrishna, Asha Manikkoth, Manimekalai, Malathy Sony Subramanian, Grüber, Gerhard
Other Authors:	School of Biological Sciences
Format:	Journal Article
Language:	English
Published:	2013
Subjects:	DRNTU::Science::Chemistry::Biochemistry DRNTU::Science::Chemistry::Crystallography
Online Access:	https://hdl.handle.net/10356/95360 http://hdl.handle.net/10220/9180

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