A portable chemotaxis platform for short and long term analysis
Flow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. Nonetheless, the general need for bulky peripherals such as mechanical pumps...
| Main Authors: | , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
|
| Online Access: | https://hdl.handle.net/10356/95401 http://hdl.handle.net/10220/9196 |
| _version_ | 1811695138920988672 |
|---|---|
| author | Poh, Yuk Kee C. O'Cearbhaill, Eoin D. Karp, Jeffrey M. Xu, Chenjie Roes, Isaac Matthiesen, Mads Emil Mu, Luye Yang, Seung Yun Miranda-Nieves, David Irimia, Daniel |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Poh, Yuk Kee C. O'Cearbhaill, Eoin D. Karp, Jeffrey M. Xu, Chenjie Roes, Isaac Matthiesen, Mads Emil Mu, Luye Yang, Seung Yun Miranda-Nieves, David Irimia, Daniel |
| author_sort | Poh, Yuk Kee C. |
| collection | NTU |
| description | Flow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. Nonetheless, the general need for bulky peripherals such as mechanical pumps and tubing and the complicated setup procedures significantly limit the widespread use of these microfluidic systems for cell migration studies. Here we present a simple method to power microfluidic devices for chemotaxis assays using the commercially available ALZET® osmotic pumps. Specifically, we developed a standalone chemotaxis platform that has the same footprint as a multiwell plate and can generate well-defined, stable chemical gradients continuously for up to 7 days. Using this platform, we validated the short-term (24 hours) and long-term (72 hours) concentration dependent PDGF-BB chemotaxis response of human bone marrow derived mesenchymal stem cells. |
| first_indexed | 2024-10-01T07:18:43Z |
| format | Journal Article |
| id | ntu-10356/95401 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:18:43Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/954012023-12-29T06:52:00Z A portable chemotaxis platform for short and long term analysis Poh, Yuk Kee C. O'Cearbhaill, Eoin D. Karp, Jeffrey M. Xu, Chenjie Roes, Isaac Matthiesen, Mads Emil Mu, Luye Yang, Seung Yun Miranda-Nieves, David Irimia, Daniel School of Chemical and Biomedical Engineering Flow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. Nonetheless, the general need for bulky peripherals such as mechanical pumps and tubing and the complicated setup procedures significantly limit the widespread use of these microfluidic systems for cell migration studies. Here we present a simple method to power microfluidic devices for chemotaxis assays using the commercially available ALZET® osmotic pumps. Specifically, we developed a standalone chemotaxis platform that has the same footprint as a multiwell plate and can generate well-defined, stable chemical gradients continuously for up to 7 days. Using this platform, we validated the short-term (24 hours) and long-term (72 hours) concentration dependent PDGF-BB chemotaxis response of human bone marrow derived mesenchymal stem cells. Published version 2013-02-20T06:37:55Z 2019-12-06T19:14:06Z 2013-02-20T06:37:55Z 2019-12-06T19:14:06Z 2012 2012 Journal Article Xu, C., Poh, Y. K. C., Roes, I., O'Cearbhaill, E. D., Matthiesen, M. E., Mu, L., et al. (2012).A portable chemotaxis platform for short and long term analysis. PLoS ONE, 7(9). 1932-6203 https://hdl.handle.net/10356/95401 http://hdl.handle.net/10220/9196 10.1371/journal.pone.0044995 23028729 en PLoS One © 2012 The Authors. application/pdf |
| spellingShingle | Poh, Yuk Kee C. O'Cearbhaill, Eoin D. Karp, Jeffrey M. Xu, Chenjie Roes, Isaac Matthiesen, Mads Emil Mu, Luye Yang, Seung Yun Miranda-Nieves, David Irimia, Daniel A portable chemotaxis platform for short and long term analysis |
| title | A portable chemotaxis platform for short and long term analysis |
| title_full | A portable chemotaxis platform for short and long term analysis |
| title_fullStr | A portable chemotaxis platform for short and long term analysis |
| title_full_unstemmed | A portable chemotaxis platform for short and long term analysis |
| title_short | A portable chemotaxis platform for short and long term analysis |
| title_sort | portable chemotaxis platform for short and long term analysis |
| url | https://hdl.handle.net/10356/95401 http://hdl.handle.net/10220/9196 |
| work_keys_str_mv | AT pohyukkeec aportablechemotaxisplatformforshortandlongtermanalysis AT ocearbhailleoind aportablechemotaxisplatformforshortandlongtermanalysis AT karpjeffreym aportablechemotaxisplatformforshortandlongtermanalysis AT xuchenjie aportablechemotaxisplatformforshortandlongtermanalysis AT roesisaac aportablechemotaxisplatformforshortandlongtermanalysis AT matthiesenmadsemil aportablechemotaxisplatformforshortandlongtermanalysis AT muluye aportablechemotaxisplatformforshortandlongtermanalysis AT yangseungyun aportablechemotaxisplatformforshortandlongtermanalysis AT mirandanievesdavid aportablechemotaxisplatformforshortandlongtermanalysis AT irimiadaniel aportablechemotaxisplatformforshortandlongtermanalysis AT pohyukkeec portablechemotaxisplatformforshortandlongtermanalysis AT ocearbhailleoind portablechemotaxisplatformforshortandlongtermanalysis AT karpjeffreym portablechemotaxisplatformforshortandlongtermanalysis AT xuchenjie portablechemotaxisplatformforshortandlongtermanalysis AT roesisaac portablechemotaxisplatformforshortandlongtermanalysis AT matthiesenmadsemil portablechemotaxisplatformforshortandlongtermanalysis AT muluye portablechemotaxisplatformforshortandlongtermanalysis AT yangseungyun portablechemotaxisplatformforshortandlongtermanalysis AT mirandanievesdavid portablechemotaxisplatformforshortandlongtermanalysis AT irimiadaniel portablechemotaxisplatformforshortandlongtermanalysis |