Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE
1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene (2) was synthesized and evaluated for specific fluorescent prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The molecule showed classic aggregation-induced emission (AIE) property in protein labeling and its quantum efficiency was further...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/95844 http://hdl.handle.net/10220/17286 |
| _version_ | 1826116710123438080 |
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| author | Yu, Yong Li, Jie Chen, Sijie Hong, Yuning Ng, Ka Ming Luo, Kathy Qian Tang, Ben Zhong |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Yu, Yong Li, Jie Chen, Sijie Hong, Yuning Ng, Ka Ming Luo, Kathy Qian Tang, Ben Zhong |
| author_sort | Yu, Yong |
| collection | NTU |
| description | 1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene (2) was synthesized and evaluated for specific fluorescent prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The molecule showed classic aggregation-induced emission (AIE) property in protein labeling and its quantum efficiency was further enhanced upon reacting with Cys. The parameters of reaction such as labeling time and concentration of dye and reducing reagent-tris(2-carboxyethyl)phosphine (TCEP) were examined to obtain the optimal labeling condition. In addition to its specific labeling effect, molecule 2 also showed its advantage over traditional self-quenching dyes through labeling Cys containing BSA with different dye/Cys ratios. |
| first_indexed | 2024-10-01T04:15:50Z |
| format | Journal Article |
| id | ntu-10356/95844 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:15:50Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/958442020-03-07T11:35:35Z Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE Yu, Yong Li, Jie Chen, Sijie Hong, Yuning Ng, Ka Ming Luo, Kathy Qian Tang, Ben Zhong School of Chemical and Biomedical Engineering 1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene (2) was synthesized and evaluated for specific fluorescent prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The molecule showed classic aggregation-induced emission (AIE) property in protein labeling and its quantum efficiency was further enhanced upon reacting with Cys. The parameters of reaction such as labeling time and concentration of dye and reducing reagent-tris(2-carboxyethyl)phosphine (TCEP) were examined to obtain the optimal labeling condition. In addition to its specific labeling effect, molecule 2 also showed its advantage over traditional self-quenching dyes through labeling Cys containing BSA with different dye/Cys ratios. 2013-11-05T06:17:22Z 2019-12-06T19:22:16Z 2013-11-05T06:17:22Z 2019-12-06T19:22:16Z 2013 2013 Journal Article Yu, Y., Li, J., Chen, S., Hong, Y., Ng, K. M., Luo, K. Q., et al. (2013). Thiol-Reactive Molecule with Dual-Emission-Enhancement Property for Specific Prestaining of Cysteine Containing Proteins in SDS-PAGE. ACS Applied Materials & Interfaces, 5(11), 4613-4616. https://hdl.handle.net/10356/95844 http://hdl.handle.net/10220/17286 10.1021/am401486h en ACS applied materials & interfaces |
| spellingShingle | Yu, Yong Li, Jie Chen, Sijie Hong, Yuning Ng, Ka Ming Luo, Kathy Qian Tang, Ben Zhong Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE |
| title | Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE |
| title_full | Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE |
| title_fullStr | Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE |
| title_full_unstemmed | Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE |
| title_short | Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE |
| title_sort | thiol reactive molecule with dual emission enhancement property for specific prestaining of cysteine containing proteins in sds page |
| url | https://hdl.handle.net/10356/95844 http://hdl.handle.net/10220/17286 |
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