Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains
A simple and specific strategy based on the bioconjugation of a photosensitizer protophophyrin IX (PpIX) with a lipopolysaccharide (LPS) binding antimicrobial peptide YI13WF (YVLWKRKRKFCFI-Amide) has been developed for the effective fluorescent imaging and photodynamic inactivation of Gram-negative...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2013
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| Online Access: | https://hdl.handle.net/10356/95930 http://hdl.handle.net/10220/10770 |
| _version_ | 1824455628679020544 |
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| author | Liu, Fang Soh, Annie Yan Ni Lim, Yingjie Mohanram, Harini Bhattacharjya, Surajit Xing, Bengang |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Liu, Fang Soh, Annie Yan Ni Lim, Yingjie Mohanram, Harini Bhattacharjya, Surajit Xing, Bengang |
| author_sort | Liu, Fang |
| collection | NTU |
| description | A simple and specific strategy based on the bioconjugation of a photosensitizer protophophyrin IX (PpIX) with a lipopolysaccharide (LPS) binding antimicrobial peptide YI13WF (YVLWKRKRKFCFI-Amide) has been developed for the effective fluorescent imaging and photodynamic inactivation of Gram-negative bacterial strains. The intracellular fluorescent imaging and photodynamic antimicrobial chemotherapy (PACT) studies supported our hypothesis that the PpIX-YI13WF conjugates could serve as efficient probes to image the bacterial strains and meanwhile indicated the potent activities against Gram-negative bacterial pathogens especially for those with antibiotics resistance when exposed to the white light irradiation. Compared to the monomeric PpIX-YI13WF conjugate, the dimeric conjugate indicated the stronger fluorescent imaging signals and higher photoinactivation toward the Gram-negative bacterial pathogens throughout the whole concentration range. In addition, the photodynamic bacterial inactivation also demonstrated more potent activity than the minimum inhibitory concentration (MIC) values of dimeric PpIX-YI13WF conjugate itself observed for E. coli DH5a (4 times), S. enterica (8 times), and other Gram-negative strains including antibiotic-resistant E. coli BL21 (8 times) and K. pneumoniae (16 times). Moreover, both fluorescent imaging and photoinactivation measurements also demonstrated that the dimeric PpIX-YI13WF conjugate could selectively recognize bacterial strains over mammalian cells and generate less photo damage to mammalian cells. We believed that the enhanced fluorescence and bacterial inactivation were probably attributed to the higher binding affinity between dimeric photosensitizer peptide conjugate and LPS components on the surface of bacterial strains, which were the results of efficient multivalent interactions. |
| first_indexed | 2025-02-19T03:41:14Z |
| format | Journal Article |
| id | ntu-10356/95930 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:41:14Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/959302020-03-07T12:18:18Z Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains Liu, Fang Soh, Annie Yan Ni Lim, Yingjie Mohanram, Harini Bhattacharjya, Surajit Xing, Bengang School of Biological Sciences School of Physical and Mathematical Sciences A simple and specific strategy based on the bioconjugation of a photosensitizer protophophyrin IX (PpIX) with a lipopolysaccharide (LPS) binding antimicrobial peptide YI13WF (YVLWKRKRKFCFI-Amide) has been developed for the effective fluorescent imaging and photodynamic inactivation of Gram-negative bacterial strains. The intracellular fluorescent imaging and photodynamic antimicrobial chemotherapy (PACT) studies supported our hypothesis that the PpIX-YI13WF conjugates could serve as efficient probes to image the bacterial strains and meanwhile indicated the potent activities against Gram-negative bacterial pathogens especially for those with antibiotics resistance when exposed to the white light irradiation. Compared to the monomeric PpIX-YI13WF conjugate, the dimeric conjugate indicated the stronger fluorescent imaging signals and higher photoinactivation toward the Gram-negative bacterial pathogens throughout the whole concentration range. In addition, the photodynamic bacterial inactivation also demonstrated more potent activity than the minimum inhibitory concentration (MIC) values of dimeric PpIX-YI13WF conjugate itself observed for E. coli DH5a (4 times), S. enterica (8 times), and other Gram-negative strains including antibiotic-resistant E. coli BL21 (8 times) and K. pneumoniae (16 times). Moreover, both fluorescent imaging and photoinactivation measurements also demonstrated that the dimeric PpIX-YI13WF conjugate could selectively recognize bacterial strains over mammalian cells and generate less photo damage to mammalian cells. We believed that the enhanced fluorescence and bacterial inactivation were probably attributed to the higher binding affinity between dimeric photosensitizer peptide conjugate and LPS components on the surface of bacterial strains, which were the results of efficient multivalent interactions. 2013-06-27T02:52:30Z 2019-12-06T19:23:27Z 2013-06-27T02:52:30Z 2019-12-06T19:23:27Z 2012 2012 Journal Article Liu, F., Soh, A. Y. N., Lim, Y., Mohanram, H., Bhattacharjya, S., & Xing, B. (2012). Lipopolysaccharide Neutralizing Peptide–Porphyrin Conjugates for Effective Photoinactivation and Intracellular Imaging of Gram-Negative Bacteria Strains. Bioconjugate Chemistry, 23(8), 1639-1647. 1043-1802 https://hdl.handle.net/10356/95930 http://hdl.handle.net/10220/10770 10.1021/bc300203d en Bioconjugate chemistry © 2012 American Chemical Society. |
| spellingShingle | Liu, Fang Soh, Annie Yan Ni Lim, Yingjie Mohanram, Harini Bhattacharjya, Surajit Xing, Bengang Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains |
| title | Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains |
| title_full | Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains |
| title_fullStr | Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains |
| title_full_unstemmed | Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains |
| title_short | Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains |
| title_sort | lipopolysaccharide neutralizing peptide porphyrin conjugates for effective photoinactivation and intracellular imaging of gram negative bacteria strains |
| url | https://hdl.handle.net/10356/95930 http://hdl.handle.net/10220/10770 |
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