An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE
A new aggregation-induced emission (AIE) compound 1,2-bis[4-(isothiocyanatomethyl)phenyl]-1,2-diphenylethene (2) was synthesized for use in SDS-PAGE. The molecule is practically nonemissive in solution but becomes highly emissive after reacting with the amine groups of the proteins by either the pre...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/95973 http://hdl.handle.net/10220/10751 |
| _version_ | 1826123545959202816 |
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| author | Yu, Yong Qin, Anjun Feng, Chao Lu, Ping Ng, Ka Ming Luo, Kathy Qian Tang, Ben Zhong |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Yu, Yong Qin, Anjun Feng, Chao Lu, Ping Ng, Ka Ming Luo, Kathy Qian Tang, Ben Zhong |
| author_sort | Yu, Yong |
| collection | NTU |
| description | A new aggregation-induced emission (AIE) compound 1,2-bis[4-(isothiocyanatomethyl)phenyl]-1,2-diphenylethene (2) was synthesized for use in SDS-PAGE. The molecule is practically nonemissive in solution but becomes highly emissive after reacting with the amine groups of the proteins by either the prestaining or poststaining method. The sensitivity of 2 achieved in the prestaining method is the same as that of Coomassie brilliant blue (CBB), while that observed in the poststaining method is higher than that of CBB. Excellent linear responses with the amount of protein were obtained in both cases. The detection of a mixture of proteins with different molecular weights was successfully achieved. |
| first_indexed | 2024-10-01T06:06:26Z |
| format | Journal Article |
| id | ntu-10356/95973 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:06:26Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/959732020-03-07T11:35:36Z An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE Yu, Yong Qin, Anjun Feng, Chao Lu, Ping Ng, Ka Ming Luo, Kathy Qian Tang, Ben Zhong School of Chemical and Biomedical Engineering A new aggregation-induced emission (AIE) compound 1,2-bis[4-(isothiocyanatomethyl)phenyl]-1,2-diphenylethene (2) was synthesized for use in SDS-PAGE. The molecule is practically nonemissive in solution but becomes highly emissive after reacting with the amine groups of the proteins by either the prestaining or poststaining method. The sensitivity of 2 achieved in the prestaining method is the same as that of Coomassie brilliant blue (CBB), while that observed in the poststaining method is higher than that of CBB. Excellent linear responses with the amount of protein were obtained in both cases. The detection of a mixture of proteins with different molecular weights was successfully achieved. 2013-06-27T01:43:44Z 2019-12-06T19:23:54Z 2013-06-27T01:43:44Z 2019-12-06T19:23:54Z 2012 2012 Journal Article Yu, Y., Qin, A., Feng, C., Lu, P., Ng, K. M., Luo, K. Q., et al. (2012). An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE. Analyst, 137(23), 5592-5596. 0003-2654 https://hdl.handle.net/10356/95973 http://hdl.handle.net/10220/10751 10.1039/c2an36002k en Analyst © 2012 The Royal Society of Chemistry. |
| spellingShingle | Yu, Yong Qin, Anjun Feng, Chao Lu, Ping Ng, Ka Ming Luo, Kathy Qian Tang, Ben Zhong An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE |
| title | An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE |
| title_full | An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE |
| title_fullStr | An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE |
| title_full_unstemmed | An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE |
| title_short | An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE |
| title_sort | amine reactive tetraphenylethylene derivative for protein detection in sds page |
| url | https://hdl.handle.net/10356/95973 http://hdl.handle.net/10220/10751 |
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