Current approaches on viral infection : proteomics and functional validations
Viruses could manipulate cellular machinery to ensure their continuous survival and thus become parasites of living organisms. Delineation of sophisticated host responses upon virus infection is a challenging task. It lies in identifying the repertoire of host factors actively involved in the viral...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/96308 http://hdl.handle.net/10220/10231 |
| _version_ | 1811681327321186304 |
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| author | Sugrue, Richard J. Zheng, Jie Tan, Boon Huan Tang, Kai |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Sugrue, Richard J. Zheng, Jie Tan, Boon Huan Tang, Kai |
| author_sort | Sugrue, Richard J. |
| collection | NTU |
| description | Viruses could manipulate cellular machinery to ensure their continuous survival and thus become parasites of living organisms. Delineation of sophisticated host responses upon virus infection is a challenging task. It lies in identifying the repertoire of host factors actively involved in the viral infectious cycle and characterizing host responses qualitatively and quantitatively during viral pathogenesis. Mass spectrometry based proteomics could be used to efficiently study pathogen-host interactions and virus-hijacked cellular signaling pathways. Moreover, direct host and viral responses upon infection could be further investigated by activity-based functional validation studies. These approaches involve drug inhibition of secretory pathway, immunofluorescence staining, dominant negative mutant of protein target, real-time PCR, small interfering siRNA-mediated knockdown, and molecular cloning studies. In this way, functional validation could gain novel insights into the high-content proteomic dataset in an unbiased and comprehensive way. |
| first_indexed | 2024-10-01T03:39:11Z |
| format | Journal Article |
| id | ntu-10356/96308 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:39:11Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/963082023-02-28T17:04:00Z Current approaches on viral infection : proteomics and functional validations Sugrue, Richard J. Zheng, Jie Tan, Boon Huan Tang, Kai School of Biological Sciences DRNTU::Science::Biological sciences Viruses could manipulate cellular machinery to ensure their continuous survival and thus become parasites of living organisms. Delineation of sophisticated host responses upon virus infection is a challenging task. It lies in identifying the repertoire of host factors actively involved in the viral infectious cycle and characterizing host responses qualitatively and quantitatively during viral pathogenesis. Mass spectrometry based proteomics could be used to efficiently study pathogen-host interactions and virus-hijacked cellular signaling pathways. Moreover, direct host and viral responses upon infection could be further investigated by activity-based functional validation studies. These approaches involve drug inhibition of secretory pathway, immunofluorescence staining, dominant negative mutant of protein target, real-time PCR, small interfering siRNA-mediated knockdown, and molecular cloning studies. In this way, functional validation could gain novel insights into the high-content proteomic dataset in an unbiased and comprehensive way. Published version 2013-06-12T03:16:37Z 2019-12-06T19:28:37Z 2013-06-12T03:16:37Z 2019-12-06T19:28:37Z 2012 2012 Journal Article Zheng, J., Tan, B. H., Sugrue, R., & Tang, K. (2012). Current approaches on viral infection: proteomics and functional validations. Frontiers in Microbiology, 3, 1-14. 1664-302X https://hdl.handle.net/10356/96308 http://hdl.handle.net/10220/10231 10.3389/fmicb.2012.00393 23162545 en Frontiers in microbiology © 2012 The Author(s). This paper was published in Frontiers in Microbiology and is made available as an electronic reprint (preprint) with permission of The Author(s). The paper can be found at the following official DOI: [http://dx.doi.org/10.3389/fmicb.2012.00393]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Sugrue, Richard J. Zheng, Jie Tan, Boon Huan Tang, Kai Current approaches on viral infection : proteomics and functional validations |
| title | Current approaches on viral infection : proteomics and functional validations |
| title_full | Current approaches on viral infection : proteomics and functional validations |
| title_fullStr | Current approaches on viral infection : proteomics and functional validations |
| title_full_unstemmed | Current approaches on viral infection : proteomics and functional validations |
| title_short | Current approaches on viral infection : proteomics and functional validations |
| title_sort | current approaches on viral infection proteomics and functional validations |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/96308 http://hdl.handle.net/10220/10231 |
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