Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass
Lignocellulosic biomass from agricultural crop residues and forest waste represents an abundant renewable resource for bioenergy and future biofuel. The current bottleneck of lignocellulosic biofuel production is the hydrolysis of biomass to sugar. To understand the enzymatic hydrolysis of complex b...
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| Format: | Journal Article |
| Language: | English |
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2013
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| Online Access: | https://hdl.handle.net/10356/96568 http://hdl.handle.net/10220/13652 |
| _version_ | 1811679718950305792 |
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| author | Ravindran, Anita Sze, Siu Kwan Adav, Sunil S. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Ravindran, Anita Sze, Siu Kwan Adav, Sunil S. |
| author_sort | Ravindran, Anita |
| collection | NTU |
| description | Lignocellulosic biomass from agricultural crop residues and forest waste represents an abundant renewable resource for bioenergy and future biofuel. The current bottleneck of lignocellulosic biofuel production is the hydrolysis of biomass to sugar. To understand the enzymatic hydrolysis of complex biomasses, in this report, lignocellulolytic enzymes secretion by Phanerochaete chrysosporium cultivated in different natural lignocellulosic biomass such as corn stover, hay, sawdust, sugarcane baggase, wheat bran and wood chips were quantitatively analyzed with the iTRAQ technique using LC-MS/MS. A diverse groups of enzymes, including cellulases, glycoside hydrolases, hemicellulases, lignin degrading enzymes, peroxidases, esterases, lipases, chitinases, peptidases, protein translocating transporter and hypothetical proteins were quantified, of which several were novel lignocellulosic biomass hydrolyzing enzymes. The quantitative expression and regulation of lignocellulolytic enzymes by P. chrysosporium were dependent on the nature and complexity of lignocellulosic biomass as well as physical size of the biomass. The iTRAQ data revealed oxidative and hydrolytic lignin degrading mechanism of P. chrysosporium. Numerous proteins presumed to be involved in natural lignocellulosic biomass transformation and degradation were expressed and produced in variable quantities in response to different agricultural and forest wastes. |
| first_indexed | 2024-10-01T03:13:37Z |
| format | Journal Article |
| id | ntu-10356/96568 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:13:37Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/965682020-03-07T12:18:18Z Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass Ravindran, Anita Sze, Siu Kwan Adav, Sunil S. School of Biological Sciences DRNTU::Science::Biological sciences Lignocellulosic biomass from agricultural crop residues and forest waste represents an abundant renewable resource for bioenergy and future biofuel. The current bottleneck of lignocellulosic biofuel production is the hydrolysis of biomass to sugar. To understand the enzymatic hydrolysis of complex biomasses, in this report, lignocellulolytic enzymes secretion by Phanerochaete chrysosporium cultivated in different natural lignocellulosic biomass such as corn stover, hay, sawdust, sugarcane baggase, wheat bran and wood chips were quantitatively analyzed with the iTRAQ technique using LC-MS/MS. A diverse groups of enzymes, including cellulases, glycoside hydrolases, hemicellulases, lignin degrading enzymes, peroxidases, esterases, lipases, chitinases, peptidases, protein translocating transporter and hypothetical proteins were quantified, of which several were novel lignocellulosic biomass hydrolyzing enzymes. The quantitative expression and regulation of lignocellulolytic enzymes by P. chrysosporium were dependent on the nature and complexity of lignocellulosic biomass as well as physical size of the biomass. The iTRAQ data revealed oxidative and hydrolytic lignin degrading mechanism of P. chrysosporium. Numerous proteins presumed to be involved in natural lignocellulosic biomass transformation and degradation were expressed and produced in variable quantities in response to different agricultural and forest wastes. 2013-09-24T07:14:50Z 2019-12-06T19:32:38Z 2013-09-24T07:14:50Z 2019-12-06T19:32:38Z 2011 2011 Journal Article Adav, S. S., Ravindran, A., & Sze, S. K. (2011). Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass. Journal of Proteomics, 75(5), 1493–1504. https://hdl.handle.net/10356/96568 http://hdl.handle.net/10220/13652 10.1016/j.jprot.2011.11.020 en Journal of proteomics |
| spellingShingle | DRNTU::Science::Biological sciences Ravindran, Anita Sze, Siu Kwan Adav, Sunil S. Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass |
| title | Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass |
| title_full | Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass |
| title_fullStr | Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass |
| title_full_unstemmed | Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass |
| title_short | Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass |
| title_sort | quantitative proteomic analysis of lignocellulolytic enzymes by phanerochaete chrysosporium on different lignocellulosic biomass |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/96568 http://hdl.handle.net/10220/13652 |
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